WANG XiFeng; CHI DeFeng; SU GuanMin; LI Lin; SHAO LiHua

doi:10.3967/0895-3988.2011.05.013

Determination of Taurine in Biological Samples by High-Performance Liquid Chromatography Using 4-Fluoro-7-Nitrobenzofurazan as a Derivatizing Agent

WANG XiFeng, CHI DeFeng, SU GuanMin, LI Lin, SHAO LiHua

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(5): 537-542

WANG XiFeng, CHI DeFeng, SU GuanMin, LI Lin, SHAO LiHua. Determination of Taurine in Biological Samples by High-Performance Liquid Chromatography Using 4-Fluoro-7-Nitrobenzofurazan as a Derivatizing Agent[J]. Biomedical and Environmental Sciences, 2011, 24(5): 537-542. doi: 10.3967/0895-3988.2011.05.013

Citation:

WANG XiFeng, CHI DeFeng, SU GuanMin, LI Lin, SHAO LiHua. Determination of Taurine in Biological Samples by High-Performance Liquid Chromatography Using 4-Fluoro-7-Nitrobenzofurazan as a Derivatizing Agent[J]. Biomedical and Environmental Sciences, 2011, 24(5): 537-542. doi: 10.3967/0895-3988.2011.05.013

doi: 10.3967/0895-3988.2011.05.013

Determination of Taurine in Biological Samples by High-Performance Liquid Chromatography Using 4-Fluoro-7-Nitrobenzofurazan as a Derivatizing Agent

Beijing Daxing Center for Disease Control and Prevention, Beijing 102600, China;Department of Hygiene Analytical Chemistry, School of Public Health, Shandong University, Jinan 250012, Shandong, China
Department of Hygiene Analytical Chemistry, School of Public Health, Shandong University, Jinan 250012, Shandong, China

摘要: Objective A highly sensitive and rapid high-performance liquid chromatography method with pre-column derivatization with 4-fluoro-7-nitrobenzofurazan was developed for determination of taurine in biological samples,including plasma,brain,and liver.Methods The optimum derivatization reaction temperature was 70℃,and at this temperature the reaction was complete within 3 min.The derivatized taurine was separated using phosphate buffer (0.02 mol/L,pH 6.0):acetonitrile (84:16,v/v) as the mobile phase at a flow rate of 1.0 mL/min,and a column temperature of 25℃.The taurine derivatives were separated within 20 min (tR:14.5 min) and fluorometrically detected at 530 nm with excitation at 470 nm.Results The intra- and the inter-day coefficients of variation for the method were 5.3％ and 7.7％,respectively.The calibration curve was linear from 0.1 μmol/L to 30.0 μmol/L with a correlation coefficient of 0.9995.Conclusion This method can be used to determine the taurine contents in plasma,brain,and liver from normal rats and human plasma.
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Abstract: Objective A highly sensitive and rapid high-performance liquid chromatography method with pre-column derivatization with 4-fluoro-7-nitrobenzofurazan was developed for determination of taurine in biological samples,including plasma,brain,and liver.Methods The optimum derivatization reaction temperature was 70℃,and at this temperature the reaction was complete within 3 min.The derivatized taurine was separated using phosphate buffer (0.02 mol/L,pH 6.0):acetonitrile (84:16,v/v) as the mobile phase at a flow rate of 1.0 mL/min,and a column temperature of 25℃.The taurine derivatives were separated within 20 min (tR:14.5 min) and fluorometrically detected at 530 nm with excitation at 470 nm.Results The intra- and the inter-day coefficients of variation for the method were 5.3％ and 7.7％,respectively.The calibration curve was linear from 0.1 μmol/L to 30.0 μmol/L with a correlation coefficient of 0.9995.Conclusion This method can be used to determine the taurine contents in plasma,brain,and liver from normal rats and human plasma.
- Taurine /
- HPLC /
- 4-fluoro-7-nitrobenzofurazan /
- Derivatization

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Determination of Taurine in Biological Samples by High-Performance Liquid Chromatography Using 4-Fluoro-7-Nitrobenzofurazan as a Derivatizing Agent

doi: 10.3967/0895-3988.2011.05.013

刊出日期: 2011-10-20

关键词:

Taurine /

HPLC /

4-fluoro-7-nitrobenzofurazan /

Derivatization

摘要: Objective A highly sensitive and rapid high-performance liquid chromatography method with pre-column derivatization with 4-fluoro-7-nitrobenzofurazan was developed for determination of taurine in biological samples,including plasma,brain,and liver.Methods The optimum derivatization reaction temperature was 70℃,and at this temperature the reaction was complete within 3 min.The derivatized taurine was separated using phosphate buffer (0.02 mol/L,pH 6.0):acetonitrile (84:16,v/v) as the mobile phase at a flow rate of 1.0 mL/min,and a column temperature of 25℃.The taurine derivatives were separated within 20 min (tR:14.5 min) and fluorometrically detected at 530 nm with excitation at 470 nm.Results The intra- and the inter-day coefficients of variation for the method were 5.3％ and 7.7％,respectively.The calibration curve was linear from 0.1 μmol/L to 30.0 μmol/L with a correlation coefficient of 0.9995.Conclusion This method can be used to determine the taurine contents in plasma,brain,and liver from normal rats and human plasma.

English Abstract

Determination of Taurine in Biological Samples by High-Performance Liquid Chromatography Using 4-Fluoro-7-Nitrobenzofurazan as a Derivatizing Agent

Beijing Daxing Center for Disease Control and Prevention, Beijing 102600, China;Department of Hygiene Analytical Chemistry, School of Public Health, Shandong University, Jinan 250012, Shandong, China

Department of Hygiene Analytical Chemistry, School of Public Health, Shandong University, Jinan 250012, Shandong, China

Publish Date: 2011-10-20

Keywords:

Abstract: Objective A highly sensitive and rapid high-performance liquid chromatography method with pre-column derivatization with 4-fluoro-7-nitrobenzofurazan was developed for determination of taurine in biological samples,including plasma,brain,and liver.Methods The optimum derivatization reaction temperature was 70℃,and at this temperature the reaction was complete within 3 min.The derivatized taurine was separated using phosphate buffer (0.02 mol/L,pH 6.0):acetonitrile (84:16,v/v) as the mobile phase at a flow rate of 1.0 mL/min,and a column temperature of 25℃.The taurine derivatives were separated within 20 min (tR:14.5 min) and fluorometrically detected at 530 nm with excitation at 470 nm.Results The intra- and the inter-day coefficients of variation for the method were 5.3％ and 7.7％,respectively.The calibration curve was linear from 0.1 μmol/L to 30.0 μmol/L with a correlation coefficient of 0.9995.Conclusion This method can be used to determine the taurine contents in plasma,brain,and liver from normal rats and human plasma.

Citation:

WANG XiFeng, CHI DeFeng, SU GuanMin, LI Lin, SHAO LiHua. Determination of Taurine in Biological Samples by High-Performance Liquid Chromatography Using 4-Fluoro-7-Nitrobenzofurazan as a Derivatizing Agent[J]. Biomedical and Environmental Sciences, 2011, 24(5): 537-542. doi: 10.3967/0895-3988.2011.05.013