Daniela-Saveta POPA; Pompei BOLFA; Bela KISS; Laurian VLASE; Ramona P?LTINEAN; Anca POP; Cornel CTOI; Gianina CRIAN; Felicia LOGHIN

doi:10.3967/bes2014.021

Influence of Genista Tinctoria L or Methylparaben on Subchronic Toxicity of Bisphenol A in Rats

Daniela-Saveta POPA, Pompei BOLFA, Bela KISS, Laurian VLASE, Ramona P?LTINEAN, Anca POP, Cornel CTOI, Gianina CRIAN, Felicia LOGHIN

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(2): 85-96

Daniela-Saveta POPA, Pompei BOLFA, Bela KISS, Laurian VLASE, Ramona P?LTINEAN, Anca POP, Cornel CTOI, Gianina CRIAN, Felicia LOGHIN. Influence of Genista Tinctoria L or Methylparaben on Subchronic Toxicity of Bisphenol A in Rats[J]. Biomedical and Environmental Sciences, 2014, 27(2): 85-96. doi: 10.3967/bes2014.021

Citation:

Daniela-Saveta POPA, Pompei BOLFA, Bela KISS, Laurian VLASE, Ramona P?LTINEAN, Anca POP, Cornel CTOI, Gianina CRIAN, Felicia LOGHIN. Influence of Genista Tinctoria L or Methylparaben on Subchronic Toxicity of Bisphenol A in Rats[J]. Biomedical and Environmental Sciences, 2014, 27(2): 85-96. doi: 10.3967/bes2014.021

doi: 10.3967/bes2014.021

Influence of Genista Tinctoria L or Methylparaben on Subchronic Toxicity of Bisphenol A in Rats

Department of Toxicology, Faculty of Pharmacy, Iuliu Ha?ieganu University of Medicine and Pharmacy Cluj-Napoca, Cluj-Napoca 400012, Romania
Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca 400372, Romania
Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy,‘Iuliu Ha?ieganu’ University of Medicine and Pharmacy Cluj-Napoca,Cluj-Napoca400012, Romania
Department of Pharmaceutical Botany, Faculty of Pharmacy,‘Iuliu Ha?ieganu’ University of Medicine and Pharmacy Cluj-Napoca, Cluj-Napoca400012, Romania

摘要: ObjectiveTo evaluate the influence of an extract ofGenista tinctoria L. herba (GT) or methylparaben (MP) on histopathological changes and2 biomarkers of oxidative stress in rats subchronicly exposed to bisphenol A (BPA).
MethodsAdult female Wistar rats were orally exposed for 90 d to BPA (50 mg/kg), BPA+GT (35 mg isoflavones/kg) or BPA+MP (250 mg/kg). Plasma and tissue samples weretaken from liver, kidney, thyroid, uterus, ovary, and mammary gland after 30, 60, and 90 d of exposure respectively. Lipid peroxidation andin vivo hydroxyl radical production were evaluated byhistological analysis along withmalondialdehyde and 2,3-dihydroxybenzoic aciddetection.
ResultsTheseverity of histopathological changes in liver and kidneyswas lower afterGT treatmentthan afterBPA or BPA+MPtreatment. A minimal thyroid receptor antagonist effect was only observedafter BPA+MPtreatment.Theabnormal folliculogenesis increased in a time-dependent manner,and the number of corpus luteum decreased.No significant histological alterationswere foundin the uterus.The mammary gland displayed specific estrogen stimulation changes at all periods. Both MP and GT revealed antioxidant properties reducing lipid peroxidation and BPA-inducedhydroxyl radical generation.
ConclusionGTL. extract ameliorates the toxic effects of BPA andisprovedto haveantioxidant potential and antitoxic effect. MP has antioxidant properties, but has either no effect or exacerbates the BPA-induced histopathological changes.
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Abstract: ObjectiveTo evaluate the influence of an extract ofGenista tinctoria L. herba (GT) or methylparaben (MP) on histopathological changes and2 biomarkers of oxidative stress in rats subchronicly exposed to bisphenol A (BPA).
MethodsAdult female Wistar rats were orally exposed for 90 d to BPA (50 mg/kg), BPA+GT (35 mg isoflavones/kg) or BPA+MP (250 mg/kg). Plasma and tissue samples weretaken from liver, kidney, thyroid, uterus, ovary, and mammary gland after 30, 60, and 90 d of exposure respectively. Lipid peroxidation andin vivo hydroxyl radical production were evaluated byhistological analysis along withmalondialdehyde and 2,3-dihydroxybenzoic aciddetection.
ResultsTheseverity of histopathological changes in liver and kidneyswas lower afterGT treatmentthan afterBPA or BPA+MPtreatment. A minimal thyroid receptor antagonist effect was only observedafter BPA+MPtreatment.Theabnormal folliculogenesis increased in a time-dependent manner,and the number of corpus luteum decreased.No significant histological alterationswere foundin the uterus.The mammary gland displayed specific estrogen stimulation changes at all periods. Both MP and GT revealed antioxidant properties reducing lipid peroxidation and BPA-inducedhydroxyl radical generation.
ConclusionGTL. extract ameliorates the toxic effects of BPA andisprovedto haveantioxidant potential and antitoxic effect. MP has antioxidant properties, but has either no effect or exacerbates the BPA-induced histopathological changes.
- Food contaminant /
- Phytoestrogen /
- Morphological effect /
- Lipid peroxidation /
- Hydroxyl radical

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Influence of Genista Tinctoria L or Methylparaben on Subchronic Toxicity of Bisphenol A in Rats

doi: 10.3967/bes2014.021

刊出日期: 2014-02-20

关键词:

Food contaminant /

Phytoestrogen /

Morphological effect /

Lipid peroxidation /

Hydroxyl radical

摘要: ObjectiveTo evaluate the influence of an extract ofGenista tinctoria L. herba (GT) or methylparaben (MP) on histopathological changes and2 biomarkers of oxidative stress in rats subchronicly exposed to bisphenol A (BPA).
MethodsAdult female Wistar rats were orally exposed for 90 d to BPA (50 mg/kg), BPA+GT (35 mg isoflavones/kg) or BPA+MP (250 mg/kg). Plasma and tissue samples weretaken from liver, kidney, thyroid, uterus, ovary, and mammary gland after 30, 60, and 90 d of exposure respectively. Lipid peroxidation andin vivo hydroxyl radical production were evaluated byhistological analysis along withmalondialdehyde and 2,3-dihydroxybenzoic aciddetection.
ResultsTheseverity of histopathological changes in liver and kidneyswas lower afterGT treatmentthan afterBPA or BPA+MPtreatment. A minimal thyroid receptor antagonist effect was only observedafter BPA+MPtreatment.Theabnormal folliculogenesis increased in a time-dependent manner,and the number of corpus luteum decreased.No significant histological alterationswere foundin the uterus.The mammary gland displayed specific estrogen stimulation changes at all periods. Both MP and GT revealed antioxidant properties reducing lipid peroxidation and BPA-inducedhydroxyl radical generation.
ConclusionGTL. extract ameliorates the toxic effects of BPA andisprovedto haveantioxidant potential and antitoxic effect. MP has antioxidant properties, but has either no effect or exacerbates the BPA-induced histopathological changes.

English Abstract

Influence of Genista Tinctoria L or Methylparaben on Subchronic Toxicity of Bisphenol A in Rats

Department of Toxicology, Faculty of Pharmacy, Iuliu Ha?ieganu University of Medicine and Pharmacy Cluj-Napoca, Cluj-Napoca 400012, Romania

Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca 400372, Romania

Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy,‘Iuliu Ha?ieganu’ University of Medicine and Pharmacy Cluj-Napoca,Cluj-Napoca400012, Romania

Department of Pharmaceutical Botany, Faculty of Pharmacy,‘Iuliu Ha?ieganu’ University of Medicine and Pharmacy Cluj-Napoca, Cluj-Napoca400012, Romania

Publish Date: 2014-02-20

Keywords:

Abstract: ObjectiveTo evaluate the influence of an extract ofGenista tinctoria L. herba (GT) or methylparaben (MP) on histopathological changes and2 biomarkers of oxidative stress in rats subchronicly exposed to bisphenol A (BPA).
MethodsAdult female Wistar rats were orally exposed for 90 d to BPA (50 mg/kg), BPA+GT (35 mg isoflavones/kg) or BPA+MP (250 mg/kg). Plasma and tissue samples weretaken from liver, kidney, thyroid, uterus, ovary, and mammary gland after 30, 60, and 90 d of exposure respectively. Lipid peroxidation andin vivo hydroxyl radical production were evaluated byhistological analysis along withmalondialdehyde and 2,3-dihydroxybenzoic aciddetection.
ResultsTheseverity of histopathological changes in liver and kidneyswas lower afterGT treatmentthan afterBPA or BPA+MPtreatment. A minimal thyroid receptor antagonist effect was only observedafter BPA+MPtreatment.Theabnormal folliculogenesis increased in a time-dependent manner,and the number of corpus luteum decreased.No significant histological alterationswere foundin the uterus.The mammary gland displayed specific estrogen stimulation changes at all periods. Both MP and GT revealed antioxidant properties reducing lipid peroxidation and BPA-inducedhydroxyl radical generation.
ConclusionGTL. extract ameliorates the toxic effects of BPA andisprovedto haveantioxidant potential and antitoxic effect. MP has antioxidant properties, but has either no effect or exacerbates the BPA-induced histopathological changes.

Citation:

Daniela-Saveta POPA, Pompei BOLFA, Bela KISS, Laurian VLASE, Ramona P?LTINEAN, Anca POP, Cornel CTOI, Gianina CRIAN, Felicia LOGHIN. Influence of Genista Tinctoria L or Methylparaben on Subchronic Toxicity of Bisphenol A in Rats[J]. Biomedical and Environmental Sciences, 2014, 27(2): 85-96. doi: 10.3967/bes2014.021