CAO Yu Xi; HE Xiao Xia; FU Shi Hong; HE Ying; LI Hao; GAO Xiao Yan; LIANG Guo Dong

doi:10.3967/bes2015.019

Real-time RT-PCR Assay for the Detection of Culex flavivirus

CAO Yu Xi, HE Xiao Xia, FU Shi Hong, HE Ying, LI Hao, GAO Xiao Yan, LIANG Guo Dong

文章导航 > Biomedical and Environmental Sciences > 2015 > 28(12): 917-919

CAO Yu Xi, HE Xiao Xia, FU Shi Hong, HE Ying, LI Hao, GAO Xiao Yan, LIANG Guo Dong. Real-time RT-PCR Assay for the Detection of Culex flavivirus[J]. Biomedical and Environmental Sciences, 2015, 28(12): 917-919. doi: 10.3967/bes2015.019

Citation:

CAO Yu Xi, HE Xiao Xia, FU Shi Hong, HE Ying, LI Hao, GAO Xiao Yan, LIANG Guo Dong. Real-time RT-PCR Assay for the Detection of Culex flavivirus[J]. Biomedical and Environmental Sciences, 2015, 28(12): 917-919. doi: 10.3967/bes2015.019

doi: 10.3967/bes2015.019

基金项目: grants from the Development Grant of State Key Laboratory of Infectious Disease Prevention and Control(2012SKLID204,2015SKLID505)%the Ministry of Science and Technology of People's Republic of China(2013ZX10004101)

Real-time RT-PCR Assay for the Detection of Culex flavivirus

State Key Laboratory of Infectious Disease Prevention and Control(SKLID), Department of Viral Encephalitis, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
State Key Laboratory of Infectious Disease Prevention and Control(SKLID), Department of Viral Encephalitis, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang China

Funds: grants from the Development Grant of State Key Laboratory of Infectious Disease Prevention and Control(2012SKLID204,2015SKLID505)%the Ministry of Science and Technology of People's Republic of China(2013ZX10004101)

摘要: Based on the Culex flavivirus (CxFV) E gene sequences in GenBank,CxFV-specific primers and probes were designed for real-time reverse transcription-polymerase chain reaction (RT-qPCR).The specificity test revealed that CxFV could be detected using RT-qPCR with the specific CxFV primers and probes;other species of arboviruses were not detected.The stability test demonstrated a coefficient of variation of ＜1.5％.A quantitative standard curve for CxFV RT-qPCR was established.
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- coefficient of variation /
- species of

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Real-time RT-PCR Assay for the Detection of Culex flavivirus

doi: 10.3967/bes2015.019

刊出日期: 2015-12-20

关键词:

coefficient of variation /

species of

摘要: Based on the Culex flavivirus (CxFV) E gene sequences in GenBank,CxFV-specific primers and probes were designed for real-time reverse transcription-polymerase chain reaction (RT-qPCR).The specificity test revealed that CxFV could be detected using RT-qPCR with the specific CxFV primers and probes;other species of arboviruses were not detected.The stability test demonstrated a coefficient of variation of ＜1.5％.A quantitative standard curve for CxFV RT-qPCR was established.