ZHU WuYang; TAO Xiao Yan; LYU Xin Jun; YU Peng Cheng; LU Zhuo Zhuang

doi:10.3967/bes2016.028

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

ZHU WuYang, TAO Xiao Yan, LYU Xin Jun, YU Peng Cheng, LU Zhuo Zhuang

文章导航 > Biomedical and Environmental Sciences > 2016 > 29(3): 224-228

ZHU WuYang, TAO Xiao Yan, LYU Xin Jun, YU Peng Cheng, LU Zhuo Zhuang. Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line[J]. Biomedical and Environmental Sciences, 2016, 29(3): 224-228. doi: 10.3967/bes2016.028

Citation:

ZHU WuYang, TAO Xiao Yan, LYU Xin Jun, YU Peng Cheng, LU Zhuo Zhuang. Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line[J]. Biomedical and Environmental Sciences, 2016, 29(3): 224-228. doi: 10.3967/bes2016.028

doi: 10.3967/bes2016.028

基金项目: China Mega-Project for Infectious Disease(2014ZX10004002-004-001)%National Natural Science Foundation of China(31500152)%andNational Key Technology R&D Program(2014BAI13B04)

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control andPrevention, Beijing 102206, China
State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Funds: China Mega-Project for Infectious Disease(2014ZX10004002-004-001)%National Natural Science Foundation of China(31500152)%andNational Key Technology R&D Program(2014BAI13B04)

摘要: The nonstructural protein 1 (NS1) of influenza A virus, which is absent from the viral particle, but highly expressed in infected cells, strongly antagonizes the interferon (IFN)-mediated antiviral response. We engineered an NS1-expressing 293 (293-NS1) cell line with no response to IFN stimulation. Compared with the parental 293 cells, the IFN-nonresponsive 293-NS1 cells improved the growth capacity of various viruses, but the introduction of NS1 barely enhanced the propagation of Tahyna virus, a negative-strand RNA virus. In particular, fastidious enteric adenovirus that replicates poorly in 293 cells may grow more efficiently in 293-NS1 cells; thus, IFN-nonresponsive 293-NS1 cells might be of great value in diagnostic laboratories for the cultivation and isolation of human enteric adenoviruses.
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- Cell Line /
- nonstructural protein /
- enteric adenovirus /
- influenza A virus /
- the growth

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Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

doi: 10.3967/bes2016.028

基金项目: China Mega-Project for Infectious Disease(2014ZX10004002-004-001)%National Natural Science Foundation of China(31500152)%andNational Key Technology R&D Program(2014BAI13B04)

刊出日期: 2016-03-20

关键词:

Cell Line /

nonstructural protein /

enteric adenovirus /

influenza A virus /

the growth

摘要: The nonstructural protein 1 (NS1) of influenza A virus, which is absent from the viral particle, but highly expressed in infected cells, strongly antagonizes the interferon (IFN)-mediated antiviral response. We engineered an NS1-expressing 293 (293-NS1) cell line with no response to IFN stimulation. Compared with the parental 293 cells, the IFN-nonresponsive 293-NS1 cells improved the growth capacity of various viruses, but the introduction of NS1 barely enhanced the propagation of Tahyna virus, a negative-strand RNA virus. In particular, fastidious enteric adenovirus that replicates poorly in 293 cells may grow more efficiently in 293-NS1 cells; thus, IFN-nonresponsive 293-NS1 cells might be of great value in diagnostic laboratories for the cultivation and isolation of human enteric adenoviruses.

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doi: 10.3967/bes2016.028

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

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Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

doi: 10.3967/bes2016.028

English Abstract

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control andPrevention, Beijing 102206, China

State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China

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doi: 10.3967/bes2016.028

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

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Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

doi: 10.3967/bes2016.028

English Abstract

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control andPrevention, Beijing 102206, China State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China

全文HTML

目录

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control andPrevention, Beijing 102206, China

State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China