2019 Vol. 32, No. 1

2019, 32(1): 0-0.
2019, 32(1): 1-1.
Original Articles
Induction of Endoplasmic Reticulum Stress by Cadmium and Its Regulation on Nrf2 Signaling Pathway in Kidneys of Rats
CHEN Zhi Jian, CHEN Jia Xing, WU Li Kang, LI Bi Yun, TIAN Ya Feng, XIAN Min, HUANG Zi Pei, YU Ri An
2019, 32(1): 1-10. doi: 10.3967/bes2019.001
Objective This study was conducted to investigate the regulation of endoplasmic reticulum stress on Nrf2 signaling pathway in the kidneys of rats. Methods Rats were divided into twelve groups of six animals each. Some groups were pre-administered with bacitracin or tauroursodeoxycholic acid (TUDCA), and all of them were treated with 5-20 μmol/kg cadmium (Cd) for 48 h. The oxidative stress levels were analyzed using kits. The mRNA and protein expression levels of endoplasmic reticulum stress-related factors and Nrf2 signaling pathway-related factors were determined using RT-PCR and western blot. Results Cd exposure resulted in oxidative stress in the kidneys of rats and upregulated the expression of endoplasmic reticulum stress (ERS)-related factors and Nrf2 signaling pathway-related factors, especially at doses of 10 and 20 μmol/kg Cd, and the expression changes were particularly obvious. Moreover, after pretreatment with bacitracin, Cd upregulated the expression of ERS-related factors to a certain extent and, at higher doses, increased the mRNA expression of Nrf2. After pretreatment with TUDCA, Cd reduced the level of ERS to a certain extent; however, at these doses, there were no significant changes in the expression of Nrf2. Conclusion Cadmium can result in ERS and oxidative stress in the kidneys of rats, activate Nrf2, and upregulate the transcriptional expression of phase Ⅱ detoxification enzymes under these experimental conditions. ERS has a positive regulation effect on Nrf2 signaling pathway but has little effect on the negative regulation of Nrf2 signaling pathway in cadmium toxicity.
Antioxidant and Anti-inflammatory Capacity of Ferulic Acid Released from Wheat Bran by Solid-state Fermentation of Aspergillus niger
YIN Zhi Na, WU Wen Jia, SUN Chong Zhen, LIU Hui Fan, CHEN Wen Bo, ZHAN Qi Ping, LEI Zhuo Gui, XIN Xuan, MA Juan Juan, YAO Kun, MIN Tian, ZHANG Meng Meng, WU Hui
2019, 32(1): 11-21. doi: 10.3967/bes2019.002
Objective A strain of Aspergillus niger (A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflammatory capacity of ferulic acid released from wheat bran by this A. niger strain (FA-WB) were evaluated. Methods Molecular identification techniques based on PCR analysis of specific genomic sequences were conducted; antioxidant ability was examined using oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA) assays, and erythrocyte hemolysis assays. RAW264.7 cells were used as a model to detect anti-inflammatory activity. Results The filamentous fungal isolate was identified to be A. niger. ORAC and CAA assay showed that FA-WB had better antioxidant activity than that of the ferulic acid standard. The erythrocyte hemolysis assay Results suggested that FA-WB could attenuate AAPH-induced oxidative stress through inhibition of reactive oxy gen species (ROS) generation. FA-WB could significantly restore the AAPH-induced increase in intracellular antioxidant enzyme activities to normal levels as well as inhibit the intracellular malondialdehyde formation. TNF-α, IL-6, and NO levels indicated that FA-WB can inhibit the inflammation induced by lipopolysaccharide (LPS). Conclusion Ferulic acid released from wheat bran by a new strain of A. niger had good anti-inflammatory activity and better antioxidant ability than standard ferulic acid.
Antioxidant Potential of Four Species of Natural Product and Therapeutic Strategies for Cancer through Suppression of Viability in the Human Multiple Myeloma Cell Line U266
Guesmi Fatma, Saidi Issam, Soussi Rawya, Hfaiedh Najla, Landoulsi Ahmed
2019, 32(1): 22-33. doi: 10.3967/bes2019.003
Objective This research aimed to evaluate the protective effects of bioactive compounds such as phenolic acids, flavonoids, and tannins present in four species extracted with methanol. Methods The total phenolic content of the methanolic extracts was measured spectrophotometrically. The effect of the extracts on cell viability in U266 cells was measured. The effects of extracts on free radical scavenging were assessed by the DPPH test and FRAP assay. Antibacterial effects of the natural products in this report were investigated by using the disc diffusion method. Results Our Results clearly demonstrated that the methanolic extracts were characterized by a high amount of phenolic compounds. It has been speculated that ME-TA and ME-TAl exhibit a significant (P < 0.05) and dose-dependent antiradical potential. The exposure of cells to high doses of extracts almost completely suppressed cell growth in vitro. ME-TA and ME-TAl showed significant cytotoxic effects at a concentration of 100 μg/mL in the U266 cell line. ME-TAl and ME-CF inhibited the growth of B. subtilis and S. aureus, respectively, to the same extent as 10 μg/μL of chloramphenicol at a concentration of 1 mg/mL. Conclusion Overall, these Results suggest that plants used in traditional medicine have a novel application as free radical scavengers, bacterial inhibitors and tumor suppressors.
Letters to the Editor
Perturbations in Amino Acid Metabolism in Children with Kaschin-Beck Disease: A Study of Urinary Target Metabolomics
HU Jian, WANG Yu Meng, WANG Wei Yi, ZHAO Zhi Jun, LI Qiang, WANG Li Hua
2019, 32(1): 34-37. doi: 10.3967/bes2019.004
Molecular Characterization of Human Respiratory Adenovirus Infection in Children from November 2016 to October 2017 in Xining City, China
YU Juan, LI Hong, LU Nan Nan, LEI You Ju, JIANG Shuang Ying, ZHAO Sheng Cang, RAO Hua Xiang
2019, 32(1): 38-41. doi: 10.3967/bes2019.005
Mortality Risk Attributed to Ambient Temperature in Nanjing, China
ZHANG Ying, WANG Shi Gong, ZHANG Xiao Ling, CHENG Yi Fan, TANG Can Jun
2019, 32(1): 42-46. doi: 10.3967/bes2019.006
The Effect of Silica Dust Exposure on the Serum Clara Cell Protein 16 Levels in Chinese Workers
LIU Jing, SONG Hai Yan, ZHU Bao Li, PAN Li Ping, QIAN Xiao Lian
2019, 32(1): 47-50. doi: 10.3967/bes2019.007
Demographic Characteristics and Environmental Risk Factors Exposure of Birth Defects in Pregnant Women: A Population-based Study
LIN Hong, LUO Mi Yang, LUO Jia You, ZENG Rong, LI Ya Mei, DU Qi Yun, FANG Jun Qun
2019, 32(1): 51-57. doi: 10.3967/bes2019.008
Significant Polymorphisms of Vitamin D Receptor Gene (rs2189480 and rs3847987) Related to the Risk of Type 2 Diabetes in Henan Rural Area
HAN Han, ZHAO Ming Xu, WANG Yan, WANG Jun, REN Bing Nan, GE Hui Na, WANG Teng, SUN Bei Bei, BA Yue, LI Wen Jie
2019, 32(1): 58-62. doi: 10.3967/bes2019.009
High Prevalence and Factors Contributing to Hyperhomocysteinemia, Folate Deficiency, and Vitamin B12 Deficiency among Healthy Adults in Shanghai, China
WANG Yu Heng, YAN Qing Hua, XU Ji Ying, LI Xin Jian, CHENG Min Na
2019, 32(1): 63-67. doi: 10.3967/bes2019.010
Policy Forum
Estimating the Health and Economic Outcomes of the Prevention of Mother-to-child Transmission of HIV Using a Decision Tree Model
QU Shui Ling, WANG Ai Ling, PAN Xiao Ping, WANG Qian, DOU Li Xia, ZHANG Tong
2019, 32(1): 68-74. doi: 10.3967/bes2019.011