2023 Vol. 36, No. 10

2023, 36(10)
2023, 36(10): 1-2.
Original Article
Aurora A Kinase Plays a Key Role in Mitosis Skip during Senescence Induced by Ionizing Radiation
ZHANG Xu Rui, ZHANG Tong Shan, ZHANG Ya Nan, HUA Jun Rui, WANG Ju Fang, HE Jin Peng
2023, 36(10): 903-916. doi: 10.3967/bes2023.119
  Objective  To investigate the fate and underlying mechanisms of G2 phase arrest in cancer cells elicited by ionizing radiation (IR).  Methods  Human melanoma A375 and 92-1 cells were treated with X-rays radiation or Aurora A inhibitor MLN8237 (MLN) and/or p21 depletion by small interfering RNA (siRNA). Cell cycle distribution was determined using flow cytometry and a fluorescent ubiquitin-based cell cycle indicator (FUCCI) system combined with histone H3 phosphorylation at Ser10 (pS10 H3) detection. Senescence was assessed using senescence-associated-β-galactosidase (SA-β-Gal), Ki67, and γH2AX staining. Protein expression levels were determined using western blotting.  Results  Tumor cells suffered severe DNA damage and underwent G2 arrest after IR treatment. The damaged cells did not successfully enter M phase nor were they stably blocked at G2 phase but underwent mitotic skipping and entered G1 phase as tetraploid cells, ultimately leading to senescence in G1. During this process, the p53/p21 pathway is hyperactivated. Accompanying p21 accumulation, Aurora A kinase levels declined sharply. MLN treatment confirmed that Aurora A kinase activity is essential for mitosis skipping and senescence induction.  Conclusion  Persistent p21 activation during IR-induced G2 phase blockade drives Aurora A kinase degradation, leading to senescence via mitotic skipping.
Comparison of Thyroglobulin and Thyroid Function in Pregnant Women between Counties with a Median Urinary Iodine Concentration of 100–149 µg/L and 150–249 µg/L
CHEN Di Qun, YE Ying, WU Jia Ni, LAN Ying, WANG Mu Hua, WU Xiao Yan, HE Meng, WANG Li Jin, ZHENG Xin Yi, CHEN Zhi Hui
2023, 36(10): 917-929. doi: 10.3967/bes2023.076
  Objective   This study explored whether thyroglobulin and thyroid disease prevalence rates were higher in pregnant Chinese women with a median urinary iodine concentration of 100–149 µg/L, compared with those with a median urinary iodine concentration of 150–249 μg/L maintained through sustainable universal salt iodization.  Methods   This was a cross-sectional study in which 812 healthy pregnant women were enrolled to collect samples of their household edible salt, urine, and blood during their routine antenatal care in the 18 counties in Fujian Province, China. The levels of salt iodine concentration, urinary iodine concentration (UIC), free triiodothyronine (FT3), free thyroid hormone (FT4), thyroid-stimulating hormone (TSH), thyroglobulin (Tg), thyroid peroxidase antibody and thyroglobulin antibody were assessed during the routine antenatal care visits.  Results   The median UIC (mUIC) in pregnant women was 130.8 μg/L (interquartile range = 91.5–198.1 μg/L) in the counties with an mUIC of 100–149 μg/L (Group I), and 172.0 μg/L (interquartile range = 123.5–244.4 μg/L) in the counties with an mUIC of 150–249 μg/L (Group II). Goiter prevalence and thyroid nodule detection rates showed no difference between Group I and Group II (P > 0.05). Except for FT4 values, the TSH, FT4, FT3, Tg and Tg values > 40 (μg/L) and the thyroid diseases prevalence rate (TDR) showed no significant differences between Group I and Group II (P > 0.05), whether or not iodine supplementation measures were taken.  Conclusion   Compared with an mUIC of 150–249 μg/L, not only there was no difference in thyroid morphology, but also the Tg value, rate of Tg values > 40 µg/L, and TDR were not higher in pregnant women in the counties with an mUIC of 100–149 μg/L achieved through sustainable universal salt iodization in Fujian Province, China.
Treatment Outcomes in COVID-19 Patients with Brucellosis: Case Series in Heilongjiang and Systematic Review of Literature
YANG Man Li, WANG Jing Ya, ZONG Xing Yu, GUAN Li, LI Hui Zhen, XIONG Yi Bai, LIU Yu Qin, LI Ting, JI Xin Yu, SHANG Xi Yu, ZHANG Hui Fang, GUO Yang, GONG Zhao Yuan, ZHANG Lei, TONG Lin, CHEN Ren Bo, FAN Yi Pin, QIN Jin, WANG Fang, LIN Gang, SHI Nan Nan, WANG Yan Ping, MA Yan
2023, 36(10): 930-939. doi: 10.3967/bes2023.035
  Objective  Clinical characteristics and outcome in COVID-19 with brucellosis patients has not been well demonstrated, we tried to analyze clinical outcome in local and literature COVID-19 cases with brucellosis before and after recovery.   Methods  We retrospectively collected hospitalization data of comorbid patients and prospectively followed up after discharge in Heilongjiang Infectious Disease Hospital from January 15, 2020 to April 29, 2022. Demographics, epidemiological, clinical symptoms, radiological and laboratory data, treatment medicines and outcomes, and follow up were analyzed, and findings of a systematic review were demonstrated.   Results  A total of four COVID-19 with brucellosis patients were included. One patient had active brucellosis before covid and 3 patients had nonactive brucellosis before brucellosis. The median age was 54.5 years, and all were males (100.0%). Two cases (50.0%) were moderate, and one was mild and asymptomatic, respectively. Three cases (75.0%) had at least one comorbidity (brucellosis excluded). All 4 patients were found in COVID-19 nucleic acid screening. Case C and D had only headache and fever on admission, respectively. Four cases were treated with Traditional Chinese medicine, western medicines for three cases, no adverse reaction occurred during hospitalization. All patients were cured and discharged. Moreover, one case (25.0%) had still active brucellosis without re-positive COVID-19, and other three cases (75.0%) have no symptoms of discomfort except one case fell fatigue and anxious during the follow-up period after recovery. Conducting the literature review, two similar cases have been reported in two case reports, and were both recovered, whereas, no data of follow up after recovery.   Conclusion  These cases indicate that COVID-19 patients with brucellosis had favorable outcome before and after recovery. More clinical studies should be conducted to confirm our findings.
Value of Pretreatment Inflammation-nutrition Score to Predict Non-response to Neoadjuvant Chemotherapy in Locally Advanced Rectal Cancer
ZHANG Guo Chao, XU Yan Yan, WU Ying Chao, CHENG Nuo, LIAN Rui, WANG Xin
2023, 36(10): 940-948. doi: 10.3967/bes2023.121
  Objective  To investigate the value of pretreatment inflammatory-nutritional biomarkers in predicting the pathological response of locally advanced rectal cancer (LARC) after neoadjuvant chemotherapy (nCT).  Methods  This retrospective study included eligible participants who underwent nCT followed by radical surgery. Pretreatment inflammatory nutritional biomarkers were calculated within one week prior to nCT. Correlations between biomarkers and pathological responses were analyzed. The cut-off values of the pretreatment biomarkers for predicting non-response were determined using receiver operating characteristic (ROC) curve analysis. The inflammation-nutrition score was calculated using the lymphocyte level, neutrophil-to-lymphocyte ratio (NLR), and prognostic nutritional index (PNI).  Results  A total of 235 patients were retrospectively recruited between January 2017 and September 2022. Lower lymphocyte levels, lymphocyte monocyte ratio (LMR), and PNI, and higher NLR and platelet-to-lymphocyte ratio (PLR) were observed in patients without response. Multivariate logistic regression analysis revealed that NLR could independently predict non-response to nCT in patients with LARC. The sensitivity and specificity of the inflammation-nutrition score for predicting nonresponse were 71.2% and 61.7%, respectively.  Conclusion  The pretreatment inflammation-nutrition score is a practical parameter for predicting non-response to nCT in patients with LARC. Patients with high scores were more likely to respond poorly to nCT.
Protein Containing the GGDEF Domain Affects Motility and Biofilm Formation in Vibrio cholerae and is Negatively Regulated by Fur and HapR
GAO He, MA Li Zhi, QIN Qin, CUI Yao, MA Xiao Han, ZHANG Yi Quan, KAN Biao
2023, 36(10): 949-958. doi: 10.3967/bes2023.080
  Objective  This study aimed to investigate whether the VCA0560 gene acts as an active diguanylate cyclase (DGC) in Vibrio cholerae and how its transcription is regulated by Fur and HapR.   Methods  The roles of VCA0560 was investigated by utilizing various phenotypic assays, including colony morphological characterization, crystal violet staining, Cyclic di-GMP (c-di-GMP) quantification, and swimming motility assay. The regulation of the VCA0560 gene by Fur and HapR was analyzed by luminescence assay, electrophoretic mobility shift assay, and DNase I footprinting.   Results  VCA0560 gene mutation did not affect biofilm formation, motility, and c-di-GMP synthesis in V. cholerae, and its overexpression remarkably enhanced biofilm formation and intracellular c-di-GMP level but reduced motility capacity. The transcription of the VCA0560 gene was directly repressed by Fur and the master quorum sensing regulator HapR.   Conclusion  Overexpressed VCA0560 functions as an active DGC in V. cholerae, and its transcription is repressed by Fur and HapR.
A Comprehensive View on the Progress of Organoid Research with an Emphasis on its Relevance to Disease Characterization
Chandra KISHORE, Vaishali Ji, Saurav Mallik, Ayan MUKHERJI, Namrata TOMAR, Soumen Kumar Pati, LI Ai Min, Sinthia Roy BANERJEE, Soumadip GHOSH, Raza Ali NAQVI
2023, 36(10): 959-971. doi: 10.3967/bes2023.083
Letter to the Editor
Association between Mitochondrial DNA Methylation and Hypertension Risk: A Cross-sectional Study in Chinese Northern Population
ZHAO Lei, JIA Ya Ning, LIU Qi Si Jing, LIU Zi Quan, LIN Hui Shu, SHUI Xin Ying, GUO Li Qiong, HOU Shi Ke
2023, 36(10): 972-978. doi: 10.3967/bes2023.122
Altered Expression of COMP, Collagen 1, and MMP9 in Pelvic Organ Prolapse
CHEN Yi Fei, XU Hai Nan, DUAN Yi Nan, XIA Zhi Jun
2023, 36(10): 979-982. doi: 10.3967/bes2023.123
Synthesis of TiO2 Nanospikes for Dual Antibacterial Activity
LI Ze Ping, CHEN Zhi Yuan, YANG Ming Yan, YI Wei Song
2023, 36(10): 983-986. doi: 10.3967/bes2023.124
Machine-learning-assisted Investigation into the Relationship between the Built Environment, Behavior, and Physical Health of the Elderly in China
WANG Xiao Ping, LI Ze Yan, ZHANG Meng, LIU Hong Yong
2023, 36(10): 987-990. doi: 10.3967/bes2023.125
Ambient Temperature and Risk of Outpatient Chronic Pharyngitis Visits: A Time-series Analysis in Xinxiang, China
LIANG Wen Juan, CHAO Ling, ZHAO Xiang Mei, ZHANG Rong Guang, ZHAO Rui Qing, WU Wei Dong, SONG Jie
2023, 36(10): 991-995. doi: 10.3967/bes2023.126
Nationwide Trends in the Prevalence of Cigarette and E-cigarette Smoking among Korean Adults between 2014–2021: A Representative Serial Study of 1.2 Million Individuals
Minji Kim, Wonyoung Cho, Jinseok Lee, Yong Sung Choi, Seung Geun Yeo, Young Joo Lee, Sang Youl Rhee, Chanyang Min, Dong Keon Yon
2023, 36(10): 996-998. doi: 10.3967/bes2023.127
Efforts to Promote High-quality Development in Chronic Disease Prevention and Control
2023, 36(10): 999-1000. doi: 10.3967/bes2023.128
Strengthening Tumor Prevention and Control, Building a Healthy China
2023, 36(10): 1001-1001. doi: 10.3967/bes2023.129
“Three Reductions, Three Healthy Conditions” Starts with Me
2023, 36(10): 1002-1002. doi: 10.3967/bes2023.130
Retraction Statement
2023, 36(10): 1003-1003. doi: 10.3967/bes2023.148