Volume 24 Issue 5
Oct.  2011
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QI YuHong, LIANG Jun, HUI YanPing, DING GuiRong, LIU JunYe, SU XiaoMing, GUO GuoZhen. Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses[J]. Biomedical and Environmental Sciences, 2011, 24(5): 560-568. doi: 10.3967/0895-3988.2011.01.016
Citation: QI YuHong, LIANG Jun, HUI YanPing, DING GuiRong, LIU JunYe, SU XiaoMing, GUO GuoZhen. Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses[J]. Biomedical and Environmental Sciences, 2011, 24(5): 560-568. doi: 10.3967/0895-3988.2011.01.016

Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses

doi: 10.3967/0895-3988.2011.01.016
Funds:  This study was supported by the Research Fund of the National Natural Science Foundation of China(No:60871068%30970670)
  • Objective We examined alterations in the expression of tumorigenesis-related genes in the pituitary gland of rats exposed to electromagnetic pulses (EMP).Methods The global gene expression profiles of the pituitary gland in EMP-exposed and control groups were detected by cDNA microarray analysis.We then validated and further investigated the reduced expression of two tumorigenesis-related genes,Pten,and Jund,by assessing their mRNA and protein expression by quantitative real-time-PCR,western blotting,and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.Results EMP exposure induced genome-wide gene expression changes in the rat pituitary gland.There was decreased expression of the Pten and Jund mRNAs and proteins in EMP-exposed rats compared with in unexposed control animals.Conclusion EMP exposure alters the expression of tumorigenesis-related genes in the pituitary gland.These tumorigenesis-related genes are potentially involved in the development of pituitary gland tumors in rats.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses

doi: 10.3967/0895-3988.2011.01.016
Funds:  This study was supported by the Research Fund of the National Natural Science Foundation of China(No:60871068%30970670)

Abstract: Objective We examined alterations in the expression of tumorigenesis-related genes in the pituitary gland of rats exposed to electromagnetic pulses (EMP).Methods The global gene expression profiles of the pituitary gland in EMP-exposed and control groups were detected by cDNA microarray analysis.We then validated and further investigated the reduced expression of two tumorigenesis-related genes,Pten,and Jund,by assessing their mRNA and protein expression by quantitative real-time-PCR,western blotting,and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.Results EMP exposure induced genome-wide gene expression changes in the rat pituitary gland.There was decreased expression of the Pten and Jund mRNAs and proteins in EMP-exposed rats compared with in unexposed control animals.Conclusion EMP exposure alters the expression of tumorigenesis-related genes in the pituitary gland.These tumorigenesis-related genes are potentially involved in the development of pituitary gland tumors in rats.

QI YuHong, LIANG Jun, HUI YanPing, DING GuiRong, LIU JunYe, SU XiaoMing, GUO GuoZhen. Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses[J]. Biomedical and Environmental Sciences, 2011, 24(5): 560-568. doi: 10.3967/0895-3988.2011.01.016
Citation: QI YuHong, LIANG Jun, HUI YanPing, DING GuiRong, LIU JunYe, SU XiaoMing, GUO GuoZhen. Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses[J]. Biomedical and Environmental Sciences, 2011, 24(5): 560-568. doi: 10.3967/0895-3988.2011.01.016

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