Volume 24 Issue 5
Oct.  2011
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HAN YanLing, LI Yuan, SONG Juan, WANG Ying, SHI Qi, CHEN Cao, ZHANG BaoYun, GUO Yan, LI ChaoPing, HAN Jun, DONG XiaoPing. Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy[J]. Biomedical and Environmental Sciences, 2011, 24(5): 523-529. doi: 10.3967/0895-3988.2011.05.011
Citation: HAN YanLing, LI Yuan, SONG Juan, WANG Ying, SHI Qi, CHEN Cao, ZHANG BaoYun, GUO Yan, LI ChaoPing, HAN Jun, DONG XiaoPing. Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy[J]. Biomedical and Environmental Sciences, 2011, 24(5): 523-529. doi: 10.3967/0895-3988.2011.05.011

Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy

doi: 10.3967/0895-3988.2011.05.011
Funds:  Chinese National Natural Science Foundation(Grants 30771914%30800975)%Institution Technique R&D Grant(2008EG150300)%National Basic Research Program of China (973 Program)(2007CB310505)%China Mega-Project for Infectious Disease(2009ZX10004-101%2008ZX10004-001%%2008ZX10004-002)%the SKLID Development Grant(2008SKLID102%2008SKLI0202)
  • Objective To break immune tolerance to prion (PrP) proteins using DNA vaccines.Methods Four different human prion DNA vaccine candidates were constructed based on the pcDNA3.1 vector:PrP-WT expressing wild-type PrP,Ubiq-PrP expressing PrP fused to ubiquitin,PrP-LII expressing PrP fused to the lysosomal integral membrane protein type II lysosome-targeting signal,and PrP-ER expressing PrP locating the ER.Using a prime-boost strategy,three-doses of DNA vaccine were injected intramuscularly into Balb/c mice,followed by two doses of PrP protein.Two weeks after the last immunization,sera and spleens were collected and PrP-specific humoral and cellular immune responses evaluated by ELISA and ELISPOT tests.Results Higher levels of serum PrP antibodies were detected in mice vaccinated using the strategy of DNA priming followed by protein boosting.Of these,WT-PrP,Ubiq-PrP,and PrP-LII induced significantly higher humoral responses.ELISPOT tests showed markedly increased numbers of IFN-γ-secreting T cells in mice vaccinated using the strategy of DNA priming followed by protein boosting after stimulation with recombinant PrP23-90 and PrP23-231.PrP-ER induced the strongest T-cell response.Conclusion Prion vaccines can break tolerance to PrP proteins and induce PrP-specific humoral and cellular immune responses.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy

doi: 10.3967/0895-3988.2011.05.011
Funds:  Chinese National Natural Science Foundation(Grants 30771914%30800975)%Institution Technique R&D Grant(2008EG150300)%National Basic Research Program of China (973 Program)(2007CB310505)%China Mega-Project for Infectious Disease(2009ZX10004-101%2008ZX10004-001%%2008ZX10004-002)%the SKLID Development Grant(2008SKLID102%2008SKLI0202)

Abstract: Objective To break immune tolerance to prion (PrP) proteins using DNA vaccines.Methods Four different human prion DNA vaccine candidates were constructed based on the pcDNA3.1 vector:PrP-WT expressing wild-type PrP,Ubiq-PrP expressing PrP fused to ubiquitin,PrP-LII expressing PrP fused to the lysosomal integral membrane protein type II lysosome-targeting signal,and PrP-ER expressing PrP locating the ER.Using a prime-boost strategy,three-doses of DNA vaccine were injected intramuscularly into Balb/c mice,followed by two doses of PrP protein.Two weeks after the last immunization,sera and spleens were collected and PrP-specific humoral and cellular immune responses evaluated by ELISA and ELISPOT tests.Results Higher levels of serum PrP antibodies were detected in mice vaccinated using the strategy of DNA priming followed by protein boosting.Of these,WT-PrP,Ubiq-PrP,and PrP-LII induced significantly higher humoral responses.ELISPOT tests showed markedly increased numbers of IFN-γ-secreting T cells in mice vaccinated using the strategy of DNA priming followed by protein boosting after stimulation with recombinant PrP23-90 and PrP23-231.PrP-ER induced the strongest T-cell response.Conclusion Prion vaccines can break tolerance to PrP proteins and induce PrP-specific humoral and cellular immune responses.

HAN YanLing, LI Yuan, SONG Juan, WANG Ying, SHI Qi, CHEN Cao, ZHANG BaoYun, GUO Yan, LI ChaoPing, HAN Jun, DONG XiaoPing. Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy[J]. Biomedical and Environmental Sciences, 2011, 24(5): 523-529. doi: 10.3967/0895-3988.2011.05.011
Citation: HAN YanLing, LI Yuan, SONG Juan, WANG Ying, SHI Qi, CHEN Cao, ZHANG BaoYun, GUO Yan, LI ChaoPing, HAN Jun, DONG XiaoPing. Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy[J]. Biomedical and Environmental Sciences, 2011, 24(5): 523-529. doi: 10.3967/0895-3988.2011.05.011

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