Comparative Genomic Analysis of Gene Variations of Two Chinese Yersinia pestis Isolates from Vaccine Strain EV76

YOU Yuan Hai; WANG Peng; WANG Yan Hua; ZHANG Mao Jun; SONG Zhi Zhong; HAI Rong; YU Dong Zheng; WANG Hai Bin; DONG Xing Qi; ZHANG Jian Zhong

doi:10.3967/0895-3988.2012.04.009

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Volume 25 Issue 4

Aug. 2012

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Article Navigation > Biomedical and Environmental Sciences > 2012 > 25(4): 440-448

YOU Yuan Hai, WANG Peng, WANG Yan Hua, ZHANG Mao Jun, SONG Zhi Zhong, HAI Rong, YU Dong Zheng, WANG Hai Bin, DONG Xing Qi, ZHANG Jian Zhong. Comparative Genomic Analysis of Gene Variations of Two Chinese Yersinia pestis Isolates from Vaccine Strain EV76[J]. Biomedical and Environmental Sciences, 2012, 25(4): 440-448. doi: 10.3967/0895-3988.2012.04.009

Citation:

YOU Yuan Hai, WANG Peng, WANG Yan Hua, ZHANG Mao Jun, SONG Zhi Zhong, HAI Rong, YU Dong Zheng, WANG Hai Bin, DONG Xing Qi, ZHANG Jian Zhong. Comparative Genomic Analysis of Gene Variations of Two Chinese Yersinia pestis Isolates from Vaccine Strain EV76[J]. Biomedical and Environmental Sciences, 2012, 25(4): 440-448. doi: 10.3967/0895-3988.2012.04.009

Comparative Genomic Analysis of Gene Variations of Two Chinese Yersinia pestis Isolates from Vaccine Strain EV76

doi: 10.3967/0895-3988.2012.04.009

State key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
State key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, Yunnan, China
Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, Yunnan, China

Funds: The National High Technology Research and Development Program of China (863 Program)(2006AA2Z4A7)%The National Key Program for Infectious Diseases of China(2008ZX1004-002)%China Mega-Project for Infectious Disease(2011ZX10004-001)

Abstract

Objective To investigate genomic variations of two Chinese Yersinia pestis isolates that were isolated from different plague foci obtained from vaccine strain EV76 from the Yunnan province of China.Methods A microarray containing 12 000 probes covering the entire genome of seven Yersinia pestis and two Yersinia pseudotuberculosis strains,was used.PCR assays were performed to confirm microarray results.Results The gene variations detected included the absence of five genes related to the synthesis of betaine in both EV76 and another sequenced attenuated strain,KIM D27.Several genes related to phage-related membrane proteins were found to be absent in the Antiqua biovar Yunnan strain,485,which was isolated from a rodent plague foci.Conclusion These findings provide initial insight into the distinct strains isolated from natural foci,within their genomic context,including Yunnan Y.pestis strains.This information will be used therefore to establish subsequent comparisons of these sequences with published complete genomes of other strains.
- arrayCGH,
- Yersinia pestis
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Comparative Genomic Analysis of Gene Variations of Two Chinese Yersinia pestis Isolates from Vaccine Strain EV76

doi: 10.3967/0895-3988.2012.04.009

State key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

State key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, Yunnan, China

Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, Yunnan, China

Key words:

arrayCGH /
Yersinia pestis

Abstract: Objective To investigate genomic variations of two Chinese Yersinia pestis isolates that were isolated from different plague foci obtained from vaccine strain EV76 from the Yunnan province of China.Methods A microarray containing 12 000 probes covering the entire genome of seven Yersinia pestis and two Yersinia pseudotuberculosis strains,was used.PCR assays were performed to confirm microarray results.Results The gene variations detected included the absence of five genes related to the synthesis of betaine in both EV76 and another sequenced attenuated strain,KIM D27.Several genes related to phage-related membrane proteins were found to be absent in the Antiqua biovar Yunnan strain,485,which was isolated from a rodent plague foci.Conclusion These findings provide initial insight into the distinct strains isolated from natural foci,within their genomic context,including Yunnan Y.pestis strains.This information will be used therefore to establish subsequent comparisons of these sequences with published complete genomes of other strains.

Citation:

YOU Yuan Hai, WANG Peng, WANG Yan Hua, ZHANG Mao Jun, SONG Zhi Zhong, HAI Rong, YU Dong Zheng, WANG Hai Bin, DONG Xing Qi, ZHANG Jian Zhong. Comparative Genomic Analysis of Gene Variations of Two Chinese Yersinia pestis Isolates from Vaccine Strain EV76[J]. Biomedical and Environmental Sciences, 2012, 25(4): 440-448. doi: 10.3967/0895-3988.2012.04.009