The effect of Some Boron Derivatives on Kanamycin Resistance and Survival of E.coli and P.aeruginosa in Lake Water

Key words: 
• Boron /
• Lake water /
• Kanamycin /
• E.coli /
• P.aeruginosa

Abstract: Objective To study MIC value of 7 boron derivatives namely [Boric acid (H3BO3),Anhydrous Borax (Na2B4OT),Sodium Borate (NaBO2),Diammonium Tetraborate (NH4)2B4O7,Sodium Perborate (NaBO3),Boron Trioxide (B203),Potassium Tetraborate (K2B4O7)] on E.coli and P.aeruginosa and their effects on survival of bacteria in lake water and resistance against kanamycin antibiotic.Methods MIC values of Boron derivatives and antibiotic were studied by broth microdilution method.The effect of boron derivatives on survival of bacteria in lake water were also determined with plate count.Results Sodium perborate was determined as the most effective substance among the studied substances.Effectiveness increased as temperature increased.E.coli was more affected from P.aeruginosa in 8 mg/mL sodium perborate concentration in lake water.Moreover,it was determined that MIC value of kanamycin antibiotic decreased 200 times by especially treating P.aeruginosa with sodium perborate in lake water.However,it can be stated that this change in resistance did not arise from microorganisms.Conclusion Sodium perborate solution can be used supportedly in kanamycin antibiotic applications for P.aeruginosa.Future studies are necessary to explore the relation between sodium perborate and kanamycin which is effective on P.aeruginosa in lake water.