All-transRetinoic Acid Regulates Th1/Th2 Balance in CD4+ T cells When GATA-3 is Deficient

ZHU Yan Feng; HU Jia Zhe; ZHAO Pin Nan; LIU Lin Xi; LI Yun

doi:10.3967/0895-3988.2013.09.010

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Volume 26 Issue 9

Sep. 2013

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Article Navigation > Biomedical and Environmental Sciences > 2013 > 26(9): 774-777

ZHU Yan Feng, HU Jia Zhe, ZHAO Pin Nan, LIU Lin Xi, LI Yun. All-transRetinoic Acid Regulates Th1/Th2 Balance in CD4+ T cells When GATA-3 is Deficient[J]. Biomedical and Environmental Sciences, 2013, 26(9): 774-777. doi: 10.3967/0895-3988.2013.09.010

Citation:

ZHU Yan Feng, HU Jia Zhe, ZHAO Pin Nan, LIU Lin Xi, LI Yun. All-transRetinoic Acid Regulates Th1/Th2 Balance in CD4+ T cells When GATA-3 is Deficient[J]. Biomedical and Environmental Sciences, 2013, 26(9): 774-777. doi: 10.3967/0895-3988.2013.09.010

All-transRetinoic Acid Regulates Th1/Th2 Balance in CD4+ T cells When GATA-3 is Deficient

doi: 10.3967/0895-3988.2013.09.010

Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan, China
Food and Drug Administration, Guangzhou 510670, China
Chengdu Centers for Diseases Control and Prevention, Chengdu 610000, Sichuan, China

Funds: This study was supportedby the National Natural Science Foundation of China (30671761)

Abstract

The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on Th1-Th2 balance modulation.However,it is unclear whether or not vitamin A can regulate Th1-Th2 balance under a strong Th1-polarizing condition.Therefore,the purpose of our study was to examine the effect of vitamin A metabolite all-trans retinoic acid (ATRA)on Th1-Th2 differentiation in CD4+ T cells under GATA-3 deficiency,which can induce Th1-polarizing condition.In the present study,GATA-3 deficiency T cells were induced by siRNA and checked by real-time quantitative PCR and western blot.GATA-3 deficiency CD4+ T cells and normal CD4+ T were treated for 48 h with or without ATRA.The expression of Th1 and Th2 cytokines were detected by qPCR and ELISA.The results would contribute to clarify the knowledge of the role of vitamin A in regulating Th1-Th2 balance under some special conditions,and help to explain the mechanism of immune regulatory function of vitamin A.
- vitamin A,
- T cells,
- immune function,
- retinoic acid,
- western blot
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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All-transRetinoic Acid Regulates Th1/Th2 Balance in CD4+ T cells When GATA-3 is Deficient

doi: 10.3967/0895-3988.2013.09.010

Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan, China

Food and Drug Administration, Guangzhou 510670, China

Chengdu Centers for Diseases Control and Prevention, Chengdu 610000, Sichuan, China

Funds: This study was supportedby the National Natural Science Foundation of China (30671761)

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Abstract:

The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on Th1-Th2 balance modulation.However,it is unclear whether or not vitamin A can regulate Th1-Th2 balance under a strong Th1-polarizing condition.Therefore,the purpose of our study was to examine the effect of vitamin A metabolite all-trans retinoic acid (ATRA)on Th1-Th2 differentiation in CD4+ T cells under GATA-3 deficiency,which can induce Th1-polarizing condition.In the present study,GATA-3 deficiency T cells were induced by siRNA and checked by real-time quantitative PCR and western blot.GATA-3 deficiency CD4+ T cells and normal CD4+ T were treated for 48 h with or without ATRA.The expression of Th1 and Th2 cytokines were detected by qPCR and ELISA.The results would contribute to clarify the knowledge of the role of vitamin A in regulating Th1-Th2 balance under some special conditions,and help to explain the mechanism of immune regulatory function of vitamin A.

Citation: