Volume 27 Issue 4
Apr.  2014
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LI Ming Hua, FU Shi Hong, CHEN Wei Xin, WANG Huan Yu, CAO Yu Xi, LIANG Guo Dong. Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China[J]. Biomedical and Environmental Sciences, 2014, 27(4): 231-239. doi: 10.3967/bes2014.046
Citation: LI Ming Hua, FU Shi Hong, CHEN Wei Xin, WANG Huan Yu, CAO Yu Xi, LIANG Guo Dong. Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China[J]. Biomedical and Environmental Sciences, 2014, 27(4): 231-239. doi: 10.3967/bes2014.046

Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China

doi: 10.3967/bes2014.046
Funds:  grants from the Ministry of Science and Technology, China(2011CB504702)%National Natural Science Foundation of China(81290342)%Development Grant of State Key Laboratory for Infectious Disease Prevention and Control(2008SKLID105)
  • Objective To determine the molecular characterization of full-length genome of Japanese encephalitis virus (JEV) genotype V.
    Methods The full-length nucleotide sequences of JEV strains isolated from different locations and sources were used in sequence and phylogenetic analysis.
    Results The full-length genome of genotypes V JEV, XZ0934, and Muar strain were composed of 10 983 and 10 988 nucleotides respectively and shared a lower level of identity with JEV genotypes I-IV, ranging from 78.4% (G I, KV1899) to 79.7% (G III, JaGAr01), for the nucleotide sequences, and from 90.0%(G I, KV1899) to 91.8%(G III, JaGAr01) for the amino acid sequences. The open reading frame (ORF) of JEV genotype V spanned nucleotides 96 to 10 397 and encoded 3 433 amino acids. Interestingly, a comparison with JEV genotype I-IV revealed that 3 nucleotides (encoded with a serine residue) were inserted in the NS4A gene of JEV genotype V, and the insertion of nucleotides was also found in downstream of the ORF stop codon in 3’-untranslated region. Moreover, numerous amino acid mutations were observed in 3 functional domains of the E gene of JEV genotype V.
    Conclusion The molecular characterization of JEV genotype V is significantly different from that of the known genotypes I-IV. The mutations located in the coding region and the non-coding region may be molecular markers of JEV genotype V and warrant further studies to determine their effects on biology and immunogenicity of genotype V strains.
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Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China

doi: 10.3967/bes2014.046
Funds:  grants from the Ministry of Science and Technology, China(2011CB504702)%National Natural Science Foundation of China(81290342)%Development Grant of State Key Laboratory for Infectious Disease Prevention and Control(2008SKLID105)

Abstract: Objective To determine the molecular characterization of full-length genome of Japanese encephalitis virus (JEV) genotype V.
Methods The full-length nucleotide sequences of JEV strains isolated from different locations and sources were used in sequence and phylogenetic analysis.
Results The full-length genome of genotypes V JEV, XZ0934, and Muar strain were composed of 10 983 and 10 988 nucleotides respectively and shared a lower level of identity with JEV genotypes I-IV, ranging from 78.4% (G I, KV1899) to 79.7% (G III, JaGAr01), for the nucleotide sequences, and from 90.0%(G I, KV1899) to 91.8%(G III, JaGAr01) for the amino acid sequences. The open reading frame (ORF) of JEV genotype V spanned nucleotides 96 to 10 397 and encoded 3 433 amino acids. Interestingly, a comparison with JEV genotype I-IV revealed that 3 nucleotides (encoded with a serine residue) were inserted in the NS4A gene of JEV genotype V, and the insertion of nucleotides was also found in downstream of the ORF stop codon in 3’-untranslated region. Moreover, numerous amino acid mutations were observed in 3 functional domains of the E gene of JEV genotype V.
Conclusion The molecular characterization of JEV genotype V is significantly different from that of the known genotypes I-IV. The mutations located in the coding region and the non-coding region may be molecular markers of JEV genotype V and warrant further studies to determine their effects on biology and immunogenicity of genotype V strains.

LI Ming Hua, FU Shi Hong, CHEN Wei Xin, WANG Huan Yu, CAO Yu Xi, LIANG Guo Dong. Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China[J]. Biomedical and Environmental Sciences, 2014, 27(4): 231-239. doi: 10.3967/bes2014.046
Citation: LI Ming Hua, FU Shi Hong, CHEN Wei Xin, WANG Huan Yu, CAO Yu Xi, LIANG Guo Dong. Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China[J]. Biomedical and Environmental Sciences, 2014, 27(4): 231-239. doi: 10.3967/bes2014.046

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