Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus
- Inactivated Sendai virus (HVJ-E) /
- Apoptosis /
- Caspase /
- Mitogen-activated protein kinase (MAPK)
Abstract: ObjectiveInactivated Sendai virus particle [hemagglutinating virus of Japan envelope (HVJ-E)] has a potential oncolytic effect due to its ability to induce apoptosis in tumor cells. However, the molecular mechanism of apoptosis induction in cancer cellsmediated by HVJ-E has not been fully elucidated.This paper aims to investigate the underlying mechanism of apoptosis induction by HVJ-E in prostate cancer cells (PC3). MethodsPC3 cells were treated with HVJ-E at various MOI, and theninterferon-β (IFN-β) production, and the cell viability and apoptosis were detected by ELISA, MTT-based assay and flow cytometry, respectively. Next, the roles of Jak-Stat, MAPK and Akt pathways played in HVJ-E-induced apoptosis in PC3 cells were analyzed by immunoblot assay. To further evaluate the cytotoxic effect of HVJ-E on PC3 cells, HVJ-E was intratumorally injected into prostate cancers on BALB/c-nude mice, and the tumor volume was monitored for 36 days. ResultsHVJ-E induced IFN-β production and activatedJak-Stat signaling pathway, which resulted in the activation of caspase-8, caspase-3, and PARP in PC3 prostate cancer cells post HVJ-E treatment. Furthermore, we observed for the first time that p38 and Jnk MAPKs in PC3 cells contributed to HVJ-E-induced apoptosis. In addition,intratumoralHVJ-E treatmentdisplayed a directinhibitoryeffect in anin vivo BALB/cnude mouseprostate cancermodel. ConclusionOur findingshaveprovided novel insights into the underlying mechanismsby whichHVJ-E induces apoptosisin tumor cells.
|GAO Hui, GONG Xiao Cheng, CHEN Ze Dong, XU Xiao Shuang, ZHANG Quan, XU Xiang Ming. Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus[J]. Biomedical and Environmental Sciences, 2014, 27(7): 506-514. doi: 10.3967/bes2014.082