Volume 27 Issue 7
Jul.  2014
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GAO Hui, GONG Xiao Cheng, CHEN Ze Dong, XU Xiao Shuang, ZHANG Quan, XU Xiang Ming. Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus[J]. Biomedical and Environmental Sciences, 2014, 27(7): 506-514. doi: 10.3967/bes2014.082
Citation: GAO Hui, GONG Xiao Cheng, CHEN Ze Dong, XU Xiao Shuang, ZHANG Quan, XU Xiang Ming. Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus[J]. Biomedical and Environmental Sciences, 2014, 27(7): 506-514. doi: 10.3967/bes2014.082

Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus

doi: 10.3967/bes2014.082
Funds:  ThisworkwassupportedbyNatural Science Foundationof Jiangsu Province(BK20130445)%National Natural Science foundation of China(31302042)
  • ObjectiveInactivated Sendai virus particle [hemagglutinating virus of Japan envelope (HVJ-E)] has a potential oncolytic effect due to its ability to induce apoptosis in tumor cells. However, the molecular mechanism of apoptosis induction in cancer cellsmediated by HVJ-E has not been fully elucidated.This paper aims to investigate the underlying mechanism of apoptosis induction by HVJ-E in prostate cancer cells (PC3). MethodsPC3 cells were treated with HVJ-E at various MOI, and theninterferon-β (IFN-β) production, and the cell viability and apoptosis were detected by ELISA, MTT-based assay and flow cytometry, respectively. Next, the roles of Jak-Stat, MAPK and Akt pathways played in HVJ-E-induced apoptosis in PC3 cells were analyzed by immunoblot assay. To further evaluate the cytotoxic effect of HVJ-E on PC3 cells, HVJ-E was intratumorally injected into prostate cancers on BALB/c-nude mice, and the tumor volume was monitored for 36 days. ResultsHVJ-E induced IFN-β production and activatedJak-Stat signaling pathway, which resulted in the activation of caspase-8, caspase-3, and PARP in PC3 prostate cancer cells post HVJ-E treatment. Furthermore, we observed for the first time that p38 and Jnk MAPKs in PC3 cells contributed to HVJ-E-induced apoptosis. In addition,intratumoralHVJ-E treatmentdisplayed a directinhibitoryeffect in anin vivo BALB/cnude mouseprostate cancermodel. ConclusionOur findingshaveprovided novel insights into the underlying mechanismsby whichHVJ-E induces apoptosisin tumor cells.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus

doi: 10.3967/bes2014.082
Funds:  ThisworkwassupportedbyNatural Science Foundationof Jiangsu Province(BK20130445)%National Natural Science foundation of China(31302042)

Abstract: ObjectiveInactivated Sendai virus particle [hemagglutinating virus of Japan envelope (HVJ-E)] has a potential oncolytic effect due to its ability to induce apoptosis in tumor cells. However, the molecular mechanism of apoptosis induction in cancer cellsmediated by HVJ-E has not been fully elucidated.This paper aims to investigate the underlying mechanism of apoptosis induction by HVJ-E in prostate cancer cells (PC3). MethodsPC3 cells were treated with HVJ-E at various MOI, and theninterferon-β (IFN-β) production, and the cell viability and apoptosis were detected by ELISA, MTT-based assay and flow cytometry, respectively. Next, the roles of Jak-Stat, MAPK and Akt pathways played in HVJ-E-induced apoptosis in PC3 cells were analyzed by immunoblot assay. To further evaluate the cytotoxic effect of HVJ-E on PC3 cells, HVJ-E was intratumorally injected into prostate cancers on BALB/c-nude mice, and the tumor volume was monitored for 36 days. ResultsHVJ-E induced IFN-β production and activatedJak-Stat signaling pathway, which resulted in the activation of caspase-8, caspase-3, and PARP in PC3 prostate cancer cells post HVJ-E treatment. Furthermore, we observed for the first time that p38 and Jnk MAPKs in PC3 cells contributed to HVJ-E-induced apoptosis. In addition,intratumoralHVJ-E treatmentdisplayed a directinhibitoryeffect in anin vivo BALB/cnude mouseprostate cancermodel. ConclusionOur findingshaveprovided novel insights into the underlying mechanismsby whichHVJ-E induces apoptosisin tumor cells.

GAO Hui, GONG Xiao Cheng, CHEN Ze Dong, XU Xiao Shuang, ZHANG Quan, XU Xiang Ming. Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus[J]. Biomedical and Environmental Sciences, 2014, 27(7): 506-514. doi: 10.3967/bes2014.082
Citation: GAO Hui, GONG Xiao Cheng, CHEN Ze Dong, XU Xiao Shuang, ZHANG Quan, XU Xiang Ming. Induction of Apoptosis in Hormone-resistant Human Prostate Cancer PC3 Cells by Inactivated Sendai Virus[J]. Biomedical and Environmental Sciences, 2014, 27(7): 506-514. doi: 10.3967/bes2014.082

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