Volume 29 Issue 4
Apr.  2016
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LI Qiang, BAO Xun Di, LIU Yun, OU Xi Chao, PANG Yu, ZHAO Yan Lin. Comparison of Two Molecular Assays For Detecting Smear Negative Pulmonary Tuberculosis[J]. Biomedical and Environmental Sciences, 2016, 29(4): 248-253. doi: 10.3967/bes2016.032
Citation: LI Qiang, BAO Xun Di, LIU Yun, OU Xi Chao, PANG Yu, ZHAO Yan Lin. Comparison of Two Molecular Assays For Detecting Smear Negative Pulmonary Tuberculosis[J]. Biomedical and Environmental Sciences, 2016, 29(4): 248-253. doi: 10.3967/bes2016.032

Comparison of Two Molecular Assays For Detecting Smear Negative Pulmonary Tuberculosis

doi: 10.3967/bes2016.032
  • Objective To compare the performance of MTBDRplus V2 and Xpert MTB/RIF for detecting smear negative pulmonary tuberculosis (PTB).
    Methods Clinical PTB suspects were enrolled consecutively in Anhui Chest Hospital and Xi’an Chest Hospital from January to December in 2014. The sputum samples of smear negative PTB suspects were collected and decontaminated. The sediment was used to conduct MTBDRplus V2, Xpert MTB/RIF and drug susceptibility test (DST). All the samples with discrepant drug susceptibility result between molecular methods and phenotypic method were confirmed by DNA sequencing.
    Results A total of 1973 cases were enrolled in this study. The detection rates ofMycobacterium tuberculosiscomplex (MTBC) by MTBDRplus V2 and Xpert MTB/RIF were 27.67% and 27.98%, respectively. When setting MGIT culture result as a gold standard, the sensitivity and specificity of MTBDRplus V2 were 86.74% and 93.84%, and the sensitivity and specificity of Xpert MTB/RIF were 86.55% and 93.43%, respectively. For the detection of the resistance to rifampin, the sensitivity and specificity of MTBDRplus V2 were 94.34% and 96.62%, and the sensitivity and specificity of Xpert MTB/RIF were 88.68% and 95.96%, respectively. For the detection of the resistance to isoniazid, the sensitivity and specificity of MTBDRplus V2 were 77.38% and 98.02%, respectively.
    Conclusion MTBDRplus V2 and Xpert MTB/RIF can be used to detect MTBC in smear negative samples with satisfactory performance.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Comparison of Two Molecular Assays For Detecting Smear Negative Pulmonary Tuberculosis

doi: 10.3967/bes2016.032

Abstract: Objective To compare the performance of MTBDRplus V2 and Xpert MTB/RIF for detecting smear negative pulmonary tuberculosis (PTB).
Methods Clinical PTB suspects were enrolled consecutively in Anhui Chest Hospital and Xi’an Chest Hospital from January to December in 2014. The sputum samples of smear negative PTB suspects were collected and decontaminated. The sediment was used to conduct MTBDRplus V2, Xpert MTB/RIF and drug susceptibility test (DST). All the samples with discrepant drug susceptibility result between molecular methods and phenotypic method were confirmed by DNA sequencing.
Results A total of 1973 cases were enrolled in this study. The detection rates ofMycobacterium tuberculosiscomplex (MTBC) by MTBDRplus V2 and Xpert MTB/RIF were 27.67% and 27.98%, respectively. When setting MGIT culture result as a gold standard, the sensitivity and specificity of MTBDRplus V2 were 86.74% and 93.84%, and the sensitivity and specificity of Xpert MTB/RIF were 86.55% and 93.43%, respectively. For the detection of the resistance to rifampin, the sensitivity and specificity of MTBDRplus V2 were 94.34% and 96.62%, and the sensitivity and specificity of Xpert MTB/RIF were 88.68% and 95.96%, respectively. For the detection of the resistance to isoniazid, the sensitivity and specificity of MTBDRplus V2 were 77.38% and 98.02%, respectively.
Conclusion MTBDRplus V2 and Xpert MTB/RIF can be used to detect MTBC in smear negative samples with satisfactory performance.

LI Qiang, BAO Xun Di, LIU Yun, OU Xi Chao, PANG Yu, ZHAO Yan Lin. Comparison of Two Molecular Assays For Detecting Smear Negative Pulmonary Tuberculosis[J]. Biomedical and Environmental Sciences, 2016, 29(4): 248-253. doi: 10.3967/bes2016.032
Citation: LI Qiang, BAO Xun Di, LIU Yun, OU Xi Chao, PANG Yu, ZHAO Yan Lin. Comparison of Two Molecular Assays For Detecting Smear Negative Pulmonary Tuberculosis[J]. Biomedical and Environmental Sciences, 2016, 29(4): 248-253. doi: 10.3967/bes2016.032

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