Volume 29 Issue 7
Jul.  2016
Turn off MathJax
Article Contents

ZHANG Xu Rui, LIU Yong Ai, SUN Fang, LI He, LEI Su Wen, WANG Ju Fang. p21 is Responsible for Ionizing Radiation-induced Bypass of Mitosis[J]. Biomedical and Environmental Sciences, 2016, 29(7): 484-493. doi: 10.3967/bes2016.064
Citation: ZHANG Xu Rui, LIU Yong Ai, SUN Fang, LI He, LEI Su Wen, WANG Ju Fang. p21 is Responsible for Ionizing Radiation-induced Bypass of Mitosis[J]. Biomedical and Environmental Sciences, 2016, 29(7): 484-493. doi: 10.3967/bes2016.064

p21 is Responsible for Ionizing Radiation-induced Bypass of Mitosis

doi: 10.3967/bes2016.064
Funds:  the National Natural Science Foundation of China [No. U1232125,31270895] and the International Science & Technology Cooperation Program of China (2015DFR30940)
  • Objective To explore the role of p21 in ionizing radiation-induced changes in protein levels during the G2/M transition and long-term G2 arrest.
    Methods Protein expression levels were assessed by western blot in the human uveal melanoma 92-1 cells after treatment with ionizing radiation. Depletion of p21 was carried out by employing the siRNA technique. Cell cycle distribution was determined by flow cytometry combined with histone H3 phosphorylation at Ser28, an M-phase marker. Senescence was assessed by senescence-associated-β-galactosidase (SA-β-gal) staining combined with Ki67 staining, a cell proliferation marker.
    Results Accompanying increased p21, the protein levels of G2/M transition genes declined significantly in 92-1 cells irradiated with 5 Gy of X-rays. Furthermore, these irradiated cells were blocked at the G2 phase followed by cellular senescence. Depletion of p21 rescued radiation-induced G2 arrest as demonstrated by the upregulation of G2/M transition kinases, as well as the high expression of histone H3 phosphorylated at Ser28. Knockdown of p21 resulted in entry into mitosis of irradiated 92-1 cells. However, cells with serious DNA damage failed to undergo cytokinesis, leading to the accumulation of multinucleated cells.
    Conclusion Our results indicated that p21 was responsible for the downregulation of G2/M transition regulatory proteins and the bypass of mitosis induced by irradiation. Downregulation of p21 by siRNA resulted in G2-arrested cells entering into mitosis with serious DNA damage. This is the first report on elucidating the role of p21 in the bypass of mitosis.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(1511) PDF downloads(110) Cited by()

Proportional views
Related

p21 is Responsible for Ionizing Radiation-induced Bypass of Mitosis

doi: 10.3967/bes2016.064
Funds:  the National Natural Science Foundation of China [No. U1232125,31270895] and the International Science & Technology Cooperation Program of China (2015DFR30940)

Abstract: Objective To explore the role of p21 in ionizing radiation-induced changes in protein levels during the G2/M transition and long-term G2 arrest.
Methods Protein expression levels were assessed by western blot in the human uveal melanoma 92-1 cells after treatment with ionizing radiation. Depletion of p21 was carried out by employing the siRNA technique. Cell cycle distribution was determined by flow cytometry combined with histone H3 phosphorylation at Ser28, an M-phase marker. Senescence was assessed by senescence-associated-β-galactosidase (SA-β-gal) staining combined with Ki67 staining, a cell proliferation marker.
Results Accompanying increased p21, the protein levels of G2/M transition genes declined significantly in 92-1 cells irradiated with 5 Gy of X-rays. Furthermore, these irradiated cells were blocked at the G2 phase followed by cellular senescence. Depletion of p21 rescued radiation-induced G2 arrest as demonstrated by the upregulation of G2/M transition kinases, as well as the high expression of histone H3 phosphorylated at Ser28. Knockdown of p21 resulted in entry into mitosis of irradiated 92-1 cells. However, cells with serious DNA damage failed to undergo cytokinesis, leading to the accumulation of multinucleated cells.
Conclusion Our results indicated that p21 was responsible for the downregulation of G2/M transition regulatory proteins and the bypass of mitosis induced by irradiation. Downregulation of p21 by siRNA resulted in G2-arrested cells entering into mitosis with serious DNA damage. This is the first report on elucidating the role of p21 in the bypass of mitosis.

ZHANG Xu Rui, LIU Yong Ai, SUN Fang, LI He, LEI Su Wen, WANG Ju Fang. p21 is Responsible for Ionizing Radiation-induced Bypass of Mitosis[J]. Biomedical and Environmental Sciences, 2016, 29(7): 484-493. doi: 10.3967/bes2016.064
Citation: ZHANG Xu Rui, LIU Yong Ai, SUN Fang, LI He, LEI Su Wen, WANG Ju Fang. p21 is Responsible for Ionizing Radiation-induced Bypass of Mitosis[J]. Biomedical and Environmental Sciences, 2016, 29(7): 484-493. doi: 10.3967/bes2016.064

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return