Volume 21 Issue 2
Apr.  2008
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XIAO-NING LIU, QI YIN, HAO ZHANG, HONG ZHANG, SHEN-JUN ZHU, YING-JIE WEI, SHENG-SHOU HU. Tissue Extracts From Infarcted Myocardium of Rats in Promoting the Differentiation of Bone Marrow Stromal Cells Into Cardiomyocyte-like Cells[J]. Biomedical and Environmental Sciences, 2008, 21(2): 110-117.
Citation: XIAO-NING LIU, QI YIN, HAO ZHANG, HONG ZHANG, SHEN-JUN ZHU, YING-JIE WEI, SHENG-SHOU HU. Tissue Extracts From Infarcted Myocardium of Rats in Promoting the Differentiation of Bone Marrow Stromal Cells Into Cardiomyocyte-like Cells[J]. Biomedical and Environmental Sciences, 2008, 21(2): 110-117.

Tissue Extracts From Infarcted Myocardium of Rats in Promoting the Differentiation of Bone Marrow Stromal Cells Into Cardiomyocyte-like Cells

Funds:  国家自然科学基金(30570722)
  • Objective To investigate whether cardiac tissue extracts from rats couldmimicthe cardiacmieruenvironment and act as a natural inducer in promoting the differentiation of bone marrow stromal cells(BMSCs)into cardiomyocyms.Metheds Three kinds of tissue extract or cell lysate[infarcted myocardial tissue extract(IMTE),normal myocardial tissue extract(NMTE)and cultured neonatal myocardial lysate(NML)]were employed to induce BMSCs into cardiomyocyte-like cells.The cells were harvested at each time point for reverse transcription-polymerage chain reaction(RT-PCR)detection,immunocytochemical analysis,and transmission electron microscopy.Results After a 7-day induction,BMSCs were enlarged and polygonal in morphology.Myofilaments,striated safcomeres,Z-fines,and more mitochondia were observed under transmission electron microscope.Elevated expression leveIs of cardiac-specific genes and proteins were also confirmed by RT-PCR and immunocytochemistry.Moreover,IMTE showed a greater capacity of differentiating BMSCs into cardiomyocyte-like cells. Conclusions Cardiac tissue extracts,especially IMTE,can effectively differentiate BMSCs into cardiomyocyte-like cells.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Tissue Extracts From Infarcted Myocardium of Rats in Promoting the Differentiation of Bone Marrow Stromal Cells Into Cardiomyocyte-like Cells

Funds:  国家自然科学基金(30570722)

Abstract: Objective To investigate whether cardiac tissue extracts from rats couldmimicthe cardiacmieruenvironment and act as a natural inducer in promoting the differentiation of bone marrow stromal cells(BMSCs)into cardiomyocyms.Metheds Three kinds of tissue extract or cell lysate[infarcted myocardial tissue extract(IMTE),normal myocardial tissue extract(NMTE)and cultured neonatal myocardial lysate(NML)]were employed to induce BMSCs into cardiomyocyte-like cells.The cells were harvested at each time point for reverse transcription-polymerage chain reaction(RT-PCR)detection,immunocytochemical analysis,and transmission electron microscopy.Results After a 7-day induction,BMSCs were enlarged and polygonal in morphology.Myofilaments,striated safcomeres,Z-fines,and more mitochondia were observed under transmission electron microscope.Elevated expression leveIs of cardiac-specific genes and proteins were also confirmed by RT-PCR and immunocytochemistry.Moreover,IMTE showed a greater capacity of differentiating BMSCs into cardiomyocyte-like cells. Conclusions Cardiac tissue extracts,especially IMTE,can effectively differentiate BMSCs into cardiomyocyte-like cells.

XIAO-NING LIU, QI YIN, HAO ZHANG, HONG ZHANG, SHEN-JUN ZHU, YING-JIE WEI, SHENG-SHOU HU. Tissue Extracts From Infarcted Myocardium of Rats in Promoting the Differentiation of Bone Marrow Stromal Cells Into Cardiomyocyte-like Cells[J]. Biomedical and Environmental Sciences, 2008, 21(2): 110-117.
Citation: XIAO-NING LIU, QI YIN, HAO ZHANG, HONG ZHANG, SHEN-JUN ZHU, YING-JIE WEI, SHENG-SHOU HU. Tissue Extracts From Infarcted Myocardium of Rats in Promoting the Differentiation of Bone Marrow Stromal Cells Into Cardiomyocyte-like Cells[J]. Biomedical and Environmental Sciences, 2008, 21(2): 110-117.

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