Tissue Extracts From Infarcted Myocardium of Rats in Promoting the Differentiation of Bone Marrow Stromal Cells Into Cardiomyocyte-like Cells

Key words: 
• Bonemarrow stromal cells /
• CeUdifferentiation /
• Cardiactissue extracts /
• Myocardial infarction

Abstract: Objective To investigate whether cardiac tissue extracts from rats couldmimicthe cardiacmieruenvironment and act as a natural inducer in promoting the differentiation of bone marrow stromal cells(BMSCs)into cardiomyocyms.Metheds Three kinds of tissue extract or cell lysate[infarcted myocardial tissue extract(IMTE),normal myocardial tissue extract(NMTE)and cultured neonatal myocardial lysate(NML)]were employed to induce BMSCs into cardiomyocyte-like cells.The cells were harvested at each time point for reverse transcription-polymerage chain reaction(RT-PCR)detection,immunocytochemical analysis,and transmission electron microscopy.Results After a 7-day induction,BMSCs were enlarged and polygonal in morphology.Myofilaments,striated safcomeres,Z-fines,and more mitochondia were observed under transmission electron microscope.Elevated expression leveIs of cardiac-specific genes and proteins were also confirmed by RT-PCR and immunocytochemistry.Moreover,IMTE showed a greater capacity of differentiating BMSCs into cardiomyocyte-like cells. Conclusions Cardiac tissue extracts,especially IMTE,can effectively differentiate BMSCs into cardiomyocyte-like cells.