Volume 23 Issue 4
Aug.  2010
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YUAN HU, ZHU-JUN SHAO, XIAO-MEI YAN, BO-QING LI, FEI ZHAO, DI XIAO, JUN REN, MING-HUAN ZHENG, CHUN-XIANG FAN, LI-HUA HE, LI XU, YI-XIN GU, HAI JIANG, FENG-HUA GUO, ZHEN-WEI DAI, HONG-YU REN, MEI-JUAN LU, XIA CHEN, QING-HUA ZOU, FAN-LIANG MENG, JIAN-ZHONG ZHANG. Proteome Analysis of Neisseria meningitidis Serogroup Strains C Associated with Outbreaks in China[J]. Biomedical and Environmental Sciences, 2010, 23(4): 251-258.
Citation: YUAN HU, ZHU-JUN SHAO, XIAO-MEI YAN, BO-QING LI, FEI ZHAO, DI XIAO, JUN REN, MING-HUAN ZHENG, CHUN-XIANG FAN, LI-HUA HE, LI XU, YI-XIN GU, HAI JIANG, FENG-HUA GUO, ZHEN-WEI DAI, HONG-YU REN, MEI-JUAN LU, XIA CHEN, QING-HUA ZOU, FAN-LIANG MENG, JIAN-ZHONG ZHANG. Proteome Analysis of Neisseria meningitidis Serogroup Strains C Associated with Outbreaks in China[J]. Biomedical and Environmental Sciences, 2010, 23(4): 251-258.

Proteome Analysis of Neisseria meningitidis Serogroup Strains C Associated with Outbreaks in China

Funds:  the Key Technologies R&D Program(2005BA711A09)%the Ministry of Science and Technology, of China
  • Objective During 2003-2005, an outbreak of meningitis due to Neisseria meningitidis serogroup C occurred in China.With the aim to find strain clues result in the final epidemics, the ancestral strain 053442, a clinical isolate, and a carrier strain 053426 with different gene type were analyzed. Methods Clinical strain 053442 and carrier strain 053426 were cultured on GC agar plates under the same condition. Two-dimensional electrophoresis was performed using the pH 3-10 nonlinear IPG strips of 24 cm length, and all the protein spots were identified by matrix-assisted laser desorption/ionization time of flight spectrometry. Results 502 and 380 protein spots were identified in 053426 and 053442 respectively, relating to 266 and 202 different genes covering a wide range of cellular functions. The express volume and number of proteins involved in energy metabolism, protein synthesis and amino acid biosynthesis in 053426 were higher than in 053442. Virulence factor Opa, Opc and a series of proteins involved in pilus assembly and retraction were identified in 053442, which appear to be of primary importance in colonization and invasion of human cells. Compared to 053442, virulence protein species were less in 053426,with lower express volumes too. No Opa and Ope were detected in 053426. Conclusion The different protein expression profiles of the clinical strain 053442 and carrier strain 053426 in the present study provide some clues of the different pathogenicity of the two strains, which may account for result in the final epidemics.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Proteome Analysis of Neisseria meningitidis Serogroup Strains C Associated with Outbreaks in China

Funds:  the Key Technologies R&D Program(2005BA711A09)%the Ministry of Science and Technology, of China

Abstract: Objective During 2003-2005, an outbreak of meningitis due to Neisseria meningitidis serogroup C occurred in China.With the aim to find strain clues result in the final epidemics, the ancestral strain 053442, a clinical isolate, and a carrier strain 053426 with different gene type were analyzed. Methods Clinical strain 053442 and carrier strain 053426 were cultured on GC agar plates under the same condition. Two-dimensional electrophoresis was performed using the pH 3-10 nonlinear IPG strips of 24 cm length, and all the protein spots were identified by matrix-assisted laser desorption/ionization time of flight spectrometry. Results 502 and 380 protein spots were identified in 053426 and 053442 respectively, relating to 266 and 202 different genes covering a wide range of cellular functions. The express volume and number of proteins involved in energy metabolism, protein synthesis and amino acid biosynthesis in 053426 were higher than in 053442. Virulence factor Opa, Opc and a series of proteins involved in pilus assembly and retraction were identified in 053442, which appear to be of primary importance in colonization and invasion of human cells. Compared to 053442, virulence protein species were less in 053426,with lower express volumes too. No Opa and Ope were detected in 053426. Conclusion The different protein expression profiles of the clinical strain 053442 and carrier strain 053426 in the present study provide some clues of the different pathogenicity of the two strains, which may account for result in the final epidemics.

YUAN HU, ZHU-JUN SHAO, XIAO-MEI YAN, BO-QING LI, FEI ZHAO, DI XIAO, JUN REN, MING-HUAN ZHENG, CHUN-XIANG FAN, LI-HUA HE, LI XU, YI-XIN GU, HAI JIANG, FENG-HUA GUO, ZHEN-WEI DAI, HONG-YU REN, MEI-JUAN LU, XIA CHEN, QING-HUA ZOU, FAN-LIANG MENG, JIAN-ZHONG ZHANG. Proteome Analysis of Neisseria meningitidis Serogroup Strains C Associated with Outbreaks in China[J]. Biomedical and Environmental Sciences, 2010, 23(4): 251-258.
Citation: YUAN HU, ZHU-JUN SHAO, XIAO-MEI YAN, BO-QING LI, FEI ZHAO, DI XIAO, JUN REN, MING-HUAN ZHENG, CHUN-XIANG FAN, LI-HUA HE, LI XU, YI-XIN GU, HAI JIANG, FENG-HUA GUO, ZHEN-WEI DAI, HONG-YU REN, MEI-JUAN LU, XIA CHEN, QING-HUA ZOU, FAN-LIANG MENG, JIAN-ZHONG ZHANG. Proteome Analysis of Neisseria meningitidis Serogroup Strains C Associated with Outbreaks in China[J]. Biomedical and Environmental Sciences, 2010, 23(4): 251-258.

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