Immunoassay for Cadmium Detection and Quantification
Abstract: Objective To detect cadmium in environmental and food samples by graphite furnace atomic absorption spectroscopy (GFAAS) and inductively coupled plasma atomic emission spectroscopy (ICPAES). Methods An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed based on a cadmium-specific monoclonal antibody.IC-ELISA for cadmium in environmental and food samples was evaluated. Results IC-ELISA showed an IC50 of 45.6 μg/L with a detection limit of 1.95 μg/L for cadmium,and showed a mean recovery ranging 97.67%-107.08%.The coefficient of variations for intra- and iuterassay was 3.41%-6.61% and 4.70%-9.21%,respectively.The correlation coefficient between IC-ELISA and GFAAS was 0.998. Conclusion IC-ELISA can detect and quantify cadmium residue in environmental or food samples.
|Citation:||GONG-LIANG LIU, JU-FANG WANG, ZHI-YONG LI, SHI-ZHONG LIANG, XIAO-NING WANG. Immunoassay for Cadmium Detection and Quantification[J]. Biomedical and Environmental Sciences, 2009, 22(3): 188-193.|