LI Zong Ji; WANG Ya Na; WANG Qi; ZHAO Wei

doi:10.3967/0895-3988.2012.03.014

Echinococcus Granulosus 14-3-3 Protein: A Potential Vaccine Candidate Against Challenge with Echinococcus Granulosus in Mice

LI Zong Ji, WANG Ya Na, WANG Qi, ZHAO Wei

文章导航 > Biomedical and Environmental Sciences > 2012 > 25(3): 352-358

LI Zong Ji, WANG Ya Na, WANG Qi, ZHAO Wei. Echinococcus Granulosus 14-3-3 Protein: A Potential Vaccine Candidate Against Challenge with Echinococcus Granulosus in Mice[J]. Biomedical and Environmental Sciences, 2012, 25(3): 352-358. doi: 10.3967/0895-3988.2012.03.014

Citation:

LI Zong Ji, WANG Ya Na, WANG Qi, ZHAO Wei. Echinococcus Granulosus 14-3-3 Protein: A Potential Vaccine Candidate Against Challenge with Echinococcus Granulosus in Mice[J]. Biomedical and Environmental Sciences, 2012, 25(3): 352-358. doi: 10.3967/0895-3988.2012.03.014

doi: 10.3967/0895-3988.2012.03.014

基金项目: This work was supported by National Natural Science Foundation of China(30260105%30660176)

Echinococcus Granulosus 14-3-3 Protein: A Potential Vaccine Candidate Against Challenge with Echinococcus Granulosus in Mice

Center of Scientific Technology of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China; Department of Medical Genetics & Cell Biology, Ningxia Medical University, Yinchuan 750004,Ningxia Hui Autonomous Region, China
The Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China

Funds: This work was supported by National Natural Science Foundation of China(30260105%30660176)

摘要: Objective To investigate the protective immunity against Echinococcus granulosus in mice immunized with rEg14-3-3.Methods ICR mice were subcutaneously immunized three times with rEg14-3-3,followed by the challenge with Echinococcus granulosus protoscoleces intraperitoneally and then sacrificed after six months of post-challenge to detect the proliferation of splenocytes by MTT assay,and to measure the secretion of IL-2,IL-4,IL-10,and IFN -γ by ELISA.The rate of reduced hydatid cyst and the levels of IgE,IgG and IgG subclasses in sera were examined.Results Mice vaccinated with rEg14-3-3 and challenged with protoscoleces revealed significant protective immunity of 84.47％.ELISA analysis indicated that the immunized mice generated specific high levels of IgG and the prevailing isotypes of IgG were IgG1 and IgG2a,Splenocytes from mice immunized with rEg14-3-3 showed a significant proliferation response.The secretion of IFN-y and IL-2 increased significantly in the vaccinated mice whereas there was no significant difference in IL-4 and IL-10 levels between vaccinated and control mice.Conclusion The results indicate that the rEg14-3-3 vaccine could induce a high level of protective immunity as a promising vaccine candidate to prevent cystic echinococcosis.
- /
- /
- /
Abstract: Objective To investigate the protective immunity against Echinococcus granulosus in mice immunized with rEg14-3-3.Methods ICR mice were subcutaneously immunized three times with rEg14-3-3,followed by the challenge with Echinococcus granulosus protoscoleces intraperitoneally and then sacrificed after six months of post-challenge to detect the proliferation of splenocytes by MTT assay,and to measure the secretion of IL-2,IL-4,IL-10,and IFN -γ by ELISA.The rate of reduced hydatid cyst and the levels of IgE,IgG and IgG subclasses in sera were examined.Results Mice vaccinated with rEg14-3-3 and challenged with protoscoleces revealed significant protective immunity of 84.47％.ELISA analysis indicated that the immunized mice generated specific high levels of IgG and the prevailing isotypes of IgG were IgG1 and IgG2a,Splenocytes from mice immunized with rEg14-3-3 showed a significant proliferation response.The secretion of IFN-y and IL-2 increased significantly in the vaccinated mice whereas there was no significant difference in IL-4 and IL-10 levels between vaccinated and control mice.Conclusion The results indicate that the rEg14-3-3 vaccine could induce a high level of protective immunity as a promising vaccine candidate to prevent cystic echinococcosis.
- Eg14-3-3 /
- Echinococcus gronuiosus /
- Vaccine /
- Immunoprotection

[1]	. 2024-3 Cover . Biomedical and Environmental Sciences, 2024, 37(3): 1-1.
[2]	. 2024-3 Contents . Biomedical and Environmental Sciences, 2024, 37(3): 1-2.
[3]	Mina Mirzaee, Setareh Haghighat, Bahareh Golkaran, Fatemeh Asgarhalvaei, Rayhaneh Mirzaee, Morteza Taghizadeh, Mohammad Ali Savoji, Behzad Esfandiari, Mehdi Mahdavi. Montanide ISA-720 and Naloxone in HBsAg Vaccine Formulation: Cytokine Profiling and Monitoring of Long-Lasting Humoral Immune Responses . Biomedical and Environmental Sciences, 2022, 35(9): 792-803. doi: 10.3967/bes2022.104
[4]	XIAO Shi Qi, XU Da, DUAN Hong Yang, FAN Xue Ting, LI Gui Lian, ZHANG Wen, LI Ma Chao, HAN Na, LI Xin Yao, LI Na, ZHAO Li lan, ZHAO Xiu Qin, WAN Kang Lin, LIU Hai Can, FENG Wen Hai. Immunogenicity of Whole Mycobacterium intracellulare Proteins and Fingding on the Cross-Reactive Proteins between M. intracellulare and M. tuberculosis . Biomedical and Environmental Sciences, 2021, 34(7): 528-539. doi: 10.3967/bes2021.073
[5]	ZENG Yi, SI Yong Feng, LAN Gui Ping, WANG Zhan, ZHOU Ling, TANG Min Zhong, SJ O`Brien, LAN Jiao, ZHOU Xiang Yang, WANG Yong Li, TANG Juan, ZHOU Zhi Xiang, DU Hai Jun, LIN Hui. LMP2-DC Vaccine Elicits Specific EBV-LMP2 Response to Effectively Improve Immunotherapy in Patients with Nasopharyngeal Cancer . Biomedical and Environmental Sciences, 2020, 33(11): 849-856. doi: 10.3967/bes2020.115
[6]	LIU Li Qi, LI Zi, JIAO Ming, LU Jian, ZHOU Jian Fang, LI Xi Yan, LIU Jia, GUO Jun Feng, XIAO Ning, ZHAO Xiang, WANG Da Yan. Development and Assessment of Two Highly Pathogenic Avian Influenza (HPAI) H5N6 Candidate Vaccine Viruses for Pandemic Preparedness . Biomedical and Environmental Sciences, 2020, 33(9): 670-679. doi: 10.3967/bes2020.088
[7]	YU Yong Pei, CHEN Jiang Ting, JIANG Zhi Wei, WANG Ling, YU Cheng Kai, YAN Xiao Yan, YAO Chen, XIA Jie Lai. Modeling the Long-term Antibody Response and Duration of Immune Protection Induced by an Inactivated, Preservative-free Hepatitis A Vaccine (Healive) in Children . Biomedical and Environmental Sciences, 2020, 33(7): 484-492. doi: 10.3967/bes2020.065
[8]	SONG Qin Qin, LUO Xiao Nuan, CHI Miao Miao, SONG Juan, XIA Dong, HAN Jun. Identification of Internal Ribosomal Entry Site inside Open Reading Frame of 14-3-3β Gene . Biomedical and Environmental Sciences, 2020, 33(4): 273-276. doi: 10.3967/bes2020.037
[9]	FAN Xue Ting, WANG Yun Long, SU Qiu Dong, QIU Feng, YI Yao, JIA Zhi Yuan, WANG Da Yan, QIN Kun, ZOU Ye Ning, BI Sheng Li, SHEN Li Ping. Intranasal Immunization Using CTA1-DD as a Mucosal Adjuvant for an Inactivated Influenza Vaccine . Biomedical and Environmental Sciences, 2019, 32(7): 531-540. doi: 10.3967/bes2019.070
[10]	DING Ya Xing, MAO Nai Ying, ZHANG Yan, LEI Yue, GAO Zhi Gang, XU Wen Bo, ZHANG Ying. Measles Virus IgG Avidity Assay for Use in Identification of Measles Vaccine Failures in Tianjin, China . Biomedical and Environmental Sciences, 2019, 32(11): 804-811. doi: 10.3967/bes2019.102
[11]	XIAO Tong Yang, LIU Hai Can, LI Xiao Qin, HUANG Ming Xiang, LI Gui Lian, LI Na, YAN Yu Han, LUO Qiao, WANG Xue Zhi, LI Ma Chao, WAN Kang Lin. Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674 . Biomedical and Environmental Sciences, 2019, 32(6): 427-437. doi: 10.3967/bes2019.056
[12]	GUO San Jun, REN Shan, XIE Yong En. Evaluation of the Protective Efficacy of a Fused OmpK/Omp22 Protein Vaccine Candidate against Acinetobacter baumannii Infection in Mice . Biomedical and Environmental Sciences, 2018, 31(2): 155-158. doi: 10.3967/bes2018.019
[13]	YANG Ren, ZHU Ying, MA Jing, HAO Yan Zhe, WANG Xuan, HOU Mei Ling, LIU Li Peng, FAN Li Yun, CAO Yu Xi, ZHANG Xiao Guang, LI Xiao Jing. Neutralizing Antibody Titer Test of Ebola Recombinant Protein Vaccine and Gene Vector Vaccine pVR-GP-FC . Biomedical and Environmental Sciences, 2018, 31(10): 721-728. doi: 10.3967/bes2018.097
[14]	LIANG Pu, YI Yao, SU Qiu Dong, QIU Feng, FAN Xue Ting, LU Xue Xin, BI Sheng Li. Efficient Humoral and Cellular Immune Responses Induced by a Chimeric Virus-like Particle Displaying the Epitope of EV71 without Adjuvant . Biomedical and Environmental Sciences, 2018, 31(5): 343-350. doi: 10.3967/bes2018.045
[15]	BO Hong, DONG Li Bo, ZHANG Ye, DONG Jie, ZOU Shu Mei, GAO Rong Bao, WANG Da Yan, SHU Yue Long. H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China . Biomedical and Environmental Sciences, 2014, 27(10): 763-769. doi: 10.3967/bes2014.111
[16]	GAO Li Dong, ZHANG Hong, CAI Liang, CHEN Bo Zhong, JIANG Yong Lin, LIU Yun Zhi, LV Xin Jun, YU Peng Cheng, HU Shi Xiong, LIU Fu Qiang, LI Hao, LI Ge Ying, SHEN Xin Xin, TAO Xiao Yan, ZHANG Si Yu, LIU Jia Hui, TANG Qing, LI Jun Hua. Epidemic of Rabies and Effect of Its Vaccine against a Dog That Consecutively Attacked Ten People in One Day . Biomedical and Environmental Sciences, 2014, 27(1): 60-64. doi: 10.3967/bes2014.017
[17]	LIU Cong Shan, ZHANG Hao Bing, YIN Jian Hai, JIANG Bin, HAN Xiu Min. Echinococcus Granulosus:Suitable in vitro Protoscolices Culture Density . Biomedical and Environmental Sciences, 2013, 26(11): 912-915. doi: 10.3967/bes2013.020
[18]	YOU Yuan Hai, WANG Peng, WANG Yan Hua, ZHANG Mao Jun, SONG Zhi Zhong, HAI Rong, YU Dong Zheng, WANG Hai Bin, DONG Xing Qi, ZHANG Jian Zhong. Comparative Genomic Analysis of Gene Variations of Two Chinese Yersinia pestis Isolates from Vaccine Strain EV76 . Biomedical and Environmental Sciences, 2012, 25(4): 440-448. doi: 10.3967/0895-3988.2012.04.009
[19]	SHUI-HUA ZHANG, JIA-XU LIANG, SHU-YAN DAI, XIAO-LIN QIU, YAN-RONG YI, YUN PAN. Immunological Effect of Subunit Influenza Vaccine Entrapped by Liposomes . Biomedical and Environmental Sciences, 2009, 22(5): 388-393.
[20]	XING-LONG YANG, WEN-CHAO LIU, WU-WEI YANG, DONG ZHONG, YU-HU LIU, JING-DONG ZHANG, JIAN-HUI JIANG, SHAN-SHAN LI. Oral Immunization of Mice With Vaccine of Attenuated Salmonella typhimurium Expressing Helicobacter pylori Urease B Subunit . Biomedical and Environmental Sciences, 2005, 18(6): 411-418.

点击查看大图

计量

文章访问数: 1150
HTML全文浏览量: 280
PDF下载量: 58
被引次数: 0

Echinococcus Granulosus 14-3-3 Protein: A Potential Vaccine Candidate Against Challenge with Echinococcus Granulosus in Mice

doi: 10.3967/0895-3988.2012.03.014

基金项目: This work was supported by National Natural Science Foundation of China(30260105%30660176)

刊出日期: 2012-06-20

关键词:

Eg14-3-3 /

Echinococcus gronuiosus /

Vaccine /

Immunoprotection

摘要: Objective To investigate the protective immunity against Echinococcus granulosus in mice immunized with rEg14-3-3.Methods ICR mice were subcutaneously immunized three times with rEg14-3-3,followed by the challenge with Echinococcus granulosus protoscoleces intraperitoneally and then sacrificed after six months of post-challenge to detect the proliferation of splenocytes by MTT assay,and to measure the secretion of IL-2,IL-4,IL-10,and IFN -γ by ELISA.The rate of reduced hydatid cyst and the levels of IgE,IgG and IgG subclasses in sera were examined.Results Mice vaccinated with rEg14-3-3 and challenged with protoscoleces revealed significant protective immunity of 84.47％.ELISA analysis indicated that the immunized mice generated specific high levels of IgG and the prevailing isotypes of IgG were IgG1 and IgG2a,Splenocytes from mice immunized with rEg14-3-3 showed a significant proliferation response.The secretion of IFN-y and IL-2 increased significantly in the vaccinated mice whereas there was no significant difference in IL-4 and IL-10 levels between vaccinated and control mice.Conclusion The results indicate that the rEg14-3-3 vaccine could induce a high level of protective immunity as a promising vaccine candidate to prevent cystic echinococcosis.

English Abstract

Echinococcus Granulosus 14-3-3 Protein: A Potential Vaccine Candidate Against Challenge with Echinococcus Granulosus in Mice

Center of Scientific Technology of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China; Department of Medical Genetics & Cell Biology, Ningxia Medical University, Yinchuan 750004,Ningxia Hui Autonomous Region, China

The Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China

Funds: This work was supported by National Natural Science Foundation of China(30260105%30660176)

Publish Date: 2012-06-20

Keywords:

Abstract: Objective To investigate the protective immunity against Echinococcus granulosus in mice immunized with rEg14-3-3.Methods ICR mice were subcutaneously immunized three times with rEg14-3-3,followed by the challenge with Echinococcus granulosus protoscoleces intraperitoneally and then sacrificed after six months of post-challenge to detect the proliferation of splenocytes by MTT assay,and to measure the secretion of IL-2,IL-4,IL-10,and IFN -γ by ELISA.The rate of reduced hydatid cyst and the levels of IgE,IgG and IgG subclasses in sera were examined.Results Mice vaccinated with rEg14-3-3 and challenged with protoscoleces revealed significant protective immunity of 84.47％.ELISA analysis indicated that the immunized mice generated specific high levels of IgG and the prevailing isotypes of IgG were IgG1 and IgG2a,Splenocytes from mice immunized with rEg14-3-3 showed a significant proliferation response.The secretion of IFN-y and IL-2 increased significantly in the vaccinated mice whereas there was no significant difference in IL-4 and IL-10 levels between vaccinated and control mice.Conclusion The results indicate that the rEg14-3-3 vaccine could induce a high level of protective immunity as a promising vaccine candidate to prevent cystic echinococcosis.

Citation: