BO Hong; DONG Li Bo; ZHANG Ye; DONG Jie; ZOU Shu Mei; GAO Rong Bao; WANG Da Yan; SHU Yue Long

doi:10.3967/bes2014.111

H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China

BO Hong, DONG Li Bo, ZHANG Ye, DONG Jie, ZOU Shu Mei, GAO Rong Bao, WANG Da Yan, SHU Yue Long

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(10): 763-769

BO Hong, DONG Li Bo, ZHANG Ye, DONG Jie, ZOU Shu Mei, GAO Rong Bao, WANG Da Yan, SHU Yue Long. H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China[J]. Biomedical and Environmental Sciences, 2014, 27(10): 763-769. doi: 10.3967/bes2014.111

Citation:

BO Hong, DONG Li Bo, ZHANG Ye, DONG Jie, ZOU Shu Mei, GAO Rong Bao, WANG Da Yan, SHU Yue Long. H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China[J]. Biomedical and Environmental Sciences, 2014, 27(10): 763-769. doi: 10.3967/bes2014.111

doi: 10.3967/bes2014.111

基金项目: This study was supported by the National High Technology Research and Development Program of China (863 Program) SQ2009AA02XK1487370

H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China

Funds: This study was supported by the National High Technology Research and Development Program of China (863 Program) SQ2009AA02XK1487370

摘要: ObjectiveTo prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China. MethodsRecombinant viruses were rescued using reverse genetics. Neuraminidase (NA) and hemagglutinin (HA) segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011 viruses were amplified by RT-PCR. Multibasic amino acid cleavage site of HA was removed and ligated into the pCIpolI vector for virus rescue. The recombinant viruses were evaluated by trypsin dependent assays. Their embryonate survival and antigenicity were compared with those of the respective wild-type viruses. ResultsThe 4 recombinant viruses showed similar antigenicity compared with wild-type viruses, chickenembryo survival and trypsin-dependent characteristics. ConclusionThe 4 recombinantviruses rescued using reverse genetics meet the criteria for classification of low pathogenic avian influenza strains, thus supporting the use of them for the development of seeds and production of pre-pandemic vaccines.
- /
- /
- /
Abstract: ObjectiveTo prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China. MethodsRecombinant viruses were rescued using reverse genetics. Neuraminidase (NA) and hemagglutinin (HA) segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011 viruses were amplified by RT-PCR. Multibasic amino acid cleavage site of HA was removed and ligated into the pCIpolI vector for virus rescue. The recombinant viruses were evaluated by trypsin dependent assays. Their embryonate survival and antigenicity were compared with those of the respective wild-type viruses. ResultsThe 4 recombinant viruses showed similar antigenicity compared with wild-type viruses, chickenembryo survival and trypsin-dependent characteristics. ConclusionThe 4 recombinantviruses rescued using reverse genetics meet the criteria for classification of low pathogenic avian influenza strains, thus supporting the use of them for the development of seeds and production of pre-pandemic vaccines.
- Influenza /
- H5N1 /
- Pre-pandemic /
- Vaccine strains

[1]	ZHAO Yang, SUN De Lin, Heather C. Bouchard, ZHANG Xin Xin, WAN Gang, HAO Yi Wei, HE Shu Xin, JIANG Yu Yong, PANG Lin. Coronavirus Disease 2019 versus Influenza A in Children: An Observational Control Study in China . Biomedical and Environmental Sciences, 2020, 33(8): 614-619. doi: 10.3967/bes2020.080
[2]	Dorjiwangmo, GAN Meng Ze, DONG Ao Bo, Baimayangjin, Tashibazong, LIU Lei, LIAO Ling Jie, RUAN Yu Hua, SHAO Yi Ming, FENG Yi, XING Hui. Molecular Characterization of HIV-1-circulating Strains among Pre-Treatment Patients in Tibet . Biomedical and Environmental Sciences, 2020, 33(12): 953-958. doi: 10.3967/bes2020.132
[3]	SUN Ying, CAO Yang, LI Zi, BAI Tian, ZHANG Hong, HU Shi Xiong, LI Fang Cai, ZHAO Xiang, CHEN Yong Kun, LU Jian, LIU Li Qi, WANG Da Yan, SHU Yue Long, ZHOU Jian Fang. Cross-neutralizing Anti-hemagglutinin Antibodies Isolated from Patients Infected with Avian Influenza A (H5N1) Virus . Biomedical and Environmental Sciences, 2020, 33(2): 103-113. doi: 10.3967/bes2020.014
[4]	LIU Li Qi, LI Zi, JIAO Ming, LU Jian, ZHOU Jian Fang, LI Xi Yan, LIU Jia, GUO Jun Feng, XIAO Ning, ZHAO Xiang, WANG Da Yan. Development and Assessment of Two Highly Pathogenic Avian Influenza (HPAI) H5N6 Candidate Vaccine Viruses for Pandemic Preparedness . Biomedical and Environmental Sciences, 2020, 33(9): 670-679. doi: 10.3967/bes2020.088
[5]	SUN Qiang, LEI Na, LU Jian, GAO Rong Bao, LI Zi, LIU Li Qi, SUN Ying, GUO Jun Feng, WANG Da Yan, SHU Yue Long. Interferon-induced Transmembrane Protein 3 Prevents Acute Influenza Pathogenesis in Mice . Biomedical and Environmental Sciences, 2020, 33(5): 295-305. doi: 10.3967/bes2020.041
[6]	FAN Xue Ting, WANG Yun Long, SU Qiu Dong, QIU Feng, YI Yao, JIA Zhi Yuan, WANG Da Yan, QIN Kun, ZOU Ye Ning, BI Sheng Li, SHEN Li Ping. Intranasal Immunization Using CTA1-DD as a Mucosal Adjuvant for an Inactivated Influenza Vaccine . Biomedical and Environmental Sciences, 2019, 32(7): 531-540. doi: 10.3967/bes2019.070
[7]	ZHU Yin Chuan, ZHANG Bin, SUN Zeng Hui, WANG Xi Jing, FAN Xiao Hui, GAO Ling Xi, LIANG Ying, CHEN Xiao Yan, ZHANG Zeng Feng. Replication and Pathology of Duck Influenza Virus Subtype H9N2 in Chukar . Biomedical and Environmental Sciences, 2018, 31(4): 306-310. doi: 10.3967/bes2018.039
[8]	LAI Cheng Cai, WANG Ke Yu, CHEN Rui, ZHANG Ai Jun, GU Hong Jing, YIN Yan Bo, WANG Dong Dong, LIU Lin Lin, XING Li, TONG Yi Gang, MA Zong Juan, YANG Peng Hui, WANG Xi Liang. Characterization of Highly Pathogenic Avian Influenza H5N1 Viruses Isolated from Domestic Poultry in China . Biomedical and Environmental Sciences, 2017, 30(1): 68-74. doi: 10.3967/bes2017.009
[9]	ZHU WuYang, TAO Xiao Yan, LYU Xin Jun, YU Peng Cheng, LU Zhuo Zhuang. Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line . Biomedical and Environmental Sciences, 2016, 29(3): 224-228. doi: 10.3967/bes2016.028
[10]	YANG Peng Fei, YAN Qing Li, LIU Chun Cheng, XING Ya Dong, ZHANG Min Hui, GAO Qiang, YU Hao, YAO Hai Bo, HE Nan Jiang. Characterization of Avian Influenza A (H7N9) Virus Prevalence in Humans and Poultry in Huai′an, China：Molecular Epidemiology, Phylogenetic, and Dynamics Analyses . Biomedical and Environmental Sciences, 2016, 29(10): 742-753. doi: 10.3967/bes2016.099
[11]	YAO Yi, XU Cui Ling, SHI Jing Hong, ZHU Yun, LI Yun Fei, BAI Tian, LI Fang Cai, CAI Tao, YUAN Fan, CHEN Tao, YANG Hao, LI Wen Chao, ZHANG Heng Jiao, ZHANG Hong, SHU Yue Long. Phylogenetic and Molecular Analysis of an H7N7 Avian Influenza Virus Isolated in East Dongting Lake in 2012 . Biomedical and Environmental Sciences, 2015, 28(7): 518-526. doi: 10.3967/bes2015.074
[12]	LI Xiao Dan, ZOU Shu Mei, ZHANG Ye, BAI Tian, GAO Rong Bao, ZHANG Xin, WU Jie, SHU Yue Long. A Novel Reassortant H2N3 Influenza Virus Lsolated from China . Biomedical and Environmental Sciences, 2014, 27(4): 240-249. doi: 10.3967/bes2014.047
[13]	DONG Bei Bei, XU Cui Ling, DONG Li Bo, CHENG Hui Jian, YANG Lei, ZOU Shu Mei, CHEN Min. A Novel Reassortant H3N8 Influenza Virus Isolated from Drinking Water for Duck in a Domestic Duck Farm in Poyang Lake Area . Biomedical and Environmental Sciences, 2013, 26(7): 546-551. doi: 10.3967/0895-3988.2013.07.005
[14]	DAI Xiao Qiu, LIU Min, ZHANG Tuo Hong, YANG Xue Song, LI Song Lin, LI Xiao Guang, LI Yu Ling, KADEERBAI Hai San, WU Huang. Clinical Predictors for Diagnosing Pandemic (H1N1) 2009 and Seasonal Influenza (H3N2) in Fever Clinics in Beijing, China . Biomedical and Environmental Sciences, 2012, 25(1): 61-68. doi: 10.3967/0895-3988.2012.01.009
[15]	ZHOU Jian Fang, ZOU Shu Mei, LI Zi, WANG Min, DONG Jie, GUO Jun Feng, WEI He Jiang, WEN Le Ying, XU Hong, SHU Yue Long. Identification of Dual Receptor-binding Specific Strains of Human H5N1 Viruses in China . Biomedical and Environmental Sciences, 2012, 25(1): 104-108. doi: 10.3967/0895-3988.2012.01.015
[16]	LIU LiQi, Li Zi, ZHOU JianFang, ZHU Yun, DONG Jie, ZHAO Xiang, GUO JunFeng, SHU YueLong. Pathogenesis and Immunogenicity of an Avian H9N2 Influenza Virus Isolated from Human . Biomedical and Environmental Sciences, 2011, 24(5): 530-536. doi: 10.3967/0895-3988.2011.05.012
[17]	YIN Da Peng, ZHU Bao Ping, WANG Hua Qing, CAO Lei, WU Wen Di, JIANG Ke Yu, XIA Wei, ZHANG Guo Min, ZHENG Jing Shan, CAO Ling Sheng, LIANG Xiao Feng. Effect of Aluminum Hydroxide Adjuvant on the Immunogenicity of the 2009 Pandemic Influenza A/H1N1 Vaccine: Multi-level Modeling of Data with Repeated Measures . Biomedical and Environmental Sciences, 2011, 24(6): 624-629. doi: 10.3967/0895-3988.2011.06.006
[18]	XIAO-XING YANG, NING DU, JIAN-FANG ZHOU, ZI LI, MIN WANG, JUN-FENG GUO, DA-YAN WANG, YUE-LONG SHU. Gene Expression Profiles Comparison between 2009 Pandemic and Seasonal H1N1 Influenza Viruses in A549 Cells . Biomedical and Environmental Sciences, 2010, 23(4): 259-266.
[19]	JUN YANG, YU-GUANG WANG, YUN-LIANG XU, XIAN-LING REN, YU MAO, XING-WANG LI. A(H1N1)Influenza Pneumonia with Acute Disseminated Encephalomyelitis: A Case Report . Biomedical and Environmental Sciences, 2010, 23(4): 323-326.
[20]	SHUI-HUA ZHANG, JIA-XU LIANG, SHU-YAN DAI, XIAO-LIN QIU, YAN-RONG YI, YUN PAN. Immunological Effect of Subunit Influenza Vaccine Entrapped by Liposomes . Biomedical and Environmental Sciences, 2009, 22(5): 388-393.

点击查看大图

计量

文章访问数: 1389
HTML全文浏览量: 416
PDF下载量: 36
被引次数: 0

H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China

doi: 10.3967/bes2014.111

基金项目: This study was supported by the National High Technology Research and Development Program of China (863 Program) SQ2009AA02XK1487370

刊出日期: 2014-10-20

关键词:

Influenza /

H5N1 /

Pre-pandemic /

Vaccine strains

摘要: ObjectiveTo prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China. MethodsRecombinant viruses were rescued using reverse genetics. Neuraminidase (NA) and hemagglutinin (HA) segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011 viruses were amplified by RT-PCR. Multibasic amino acid cleavage site of HA was removed and ligated into the pCIpolI vector for virus rescue. The recombinant viruses were evaluated by trypsin dependent assays. Their embryonate survival and antigenicity were compared with those of the respective wild-type viruses. ResultsThe 4 recombinant viruses showed similar antigenicity compared with wild-type viruses, chickenembryo survival and trypsin-dependent characteristics. ConclusionThe 4 recombinantviruses rescued using reverse genetics meet the criteria for classification of low pathogenic avian influenza strains, thus supporting the use of them for the development of seeds and production of pre-pandemic vaccines.

English Abstract

H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China

Funds: This study was supported by the National High Technology Research and Development Program of China (863 Program) SQ2009AA02XK1487370

Publish Date: 2014-10-20

Keywords:

Abstract: ObjectiveTo prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China. MethodsRecombinant viruses were rescued using reverse genetics. Neuraminidase (NA) and hemagglutinin (HA) segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011 viruses were amplified by RT-PCR. Multibasic amino acid cleavage site of HA was removed and ligated into the pCIpolI vector for virus rescue. The recombinant viruses were evaluated by trypsin dependent assays. Their embryonate survival and antigenicity were compared with those of the respective wild-type viruses. ResultsThe 4 recombinant viruses showed similar antigenicity compared with wild-type viruses, chickenembryo survival and trypsin-dependent characteristics. ConclusionThe 4 recombinantviruses rescued using reverse genetics meet the criteria for classification of low pathogenic avian influenza strains, thus supporting the use of them for the development of seeds and production of pre-pandemic vaccines.

Citation: