ZHOU Mei Juan; ZHENG Li; GUO Ling; LIU Wei Ling; LV Chao; JIANG Li Hong; OU Cheng Shan; DING Zhen Hua

doi:10.3967/0895-3988.2012.05.013

Differential Responses to UVB Irradiation in Human Keratinocytes and Epidermoid Carcinoma Cells

ZHOU Mei Juan, ZHENG Li, GUO Ling, LIU Wei Ling, LV Chao, JIANG Li Hong, OU Cheng Shan, DING Zhen Hua

文章导航 > Biomedical and Environmental Sciences > 2012 > 25(5): 583-589

ZHOU Mei Juan, ZHENG Li, GUO Ling, LIU Wei Ling, LV Chao, JIANG Li Hong, OU Cheng Shan, DING Zhen Hua. Differential Responses to UVB Irradiation in Human Keratinocytes and Epidermoid Carcinoma Cells[J]. Biomedical and Environmental Sciences, 2012, 25(5): 583-589. doi: 10.3967/0895-3988.2012.05.013

Citation:

ZHOU Mei Juan, ZHENG Li, GUO Ling, LIU Wei Ling, LV Chao, JIANG Li Hong, OU Cheng Shan, DING Zhen Hua. Differential Responses to UVB Irradiation in Human Keratinocytes and Epidermoid Carcinoma Cells[J]. Biomedical and Environmental Sciences, 2012, 25(5): 583-589. doi: 10.3967/0895-3988.2012.05.013

doi: 10.3967/0895-3988.2012.05.013

基金项目: the National Natural Science Foundation of China(30970673%81172634)%Natural Science Foundation of Guangdong Province(9151022501000013%S2011040003686)%Guangdong Province "211 Project"(200826GW%201007GW)%Grant from School of Public Health and Tropical Medicine of Southern Medical University,China(Grant No.GW201111)

Differential Responses to UVB Irradiation in Human Keratinocytes and Epidermoid Carcinoma Cells

Department of Radiation Medicine,School of Public Health and Tropical Medicine,Southern Medical University,Guangzhou 510515,Guangdong,China

Funds: the National Natural Science Foundation of China(30970673%81172634)%Natural Science Foundation of Guangdong Province(9151022501000013%S2011040003686)%Guangdong Province "211 Project"(200826GW%201007GW)%Grant from School of Public Health and Tropical Medicine of Southern Medical University,China(Grant No.GW201111)

摘要: Objective To examine UVB‐induced responses in normal human keratinocytes (HaCaT) and epidermoid carcinoma cells (A431) at the cellular and molecular level, and investigated the protective effect of salidroside.Methods Cells irradiated by UVB at various dosage and their viability was assessed by MTT assays, cell cycle was analysed by flow cytometry. The expression of NF‐κB, BCL‐2, and CDK6 after 50 J/m2 UVB irradiation were detected by RT‐PCR and western blotting.Results Our results confirmed greater tolerance of A341 cells to UVB‐induced damage such as cell viability and cell cycle arrest, which was accompanied by differential expression changes in NF‐κB,BCL‐2, and CDK6. UVB exposure resulted in HaCaT cells undergoing G1‐S phase arrest. When treated with salidroside, HaCaT survival was significantly enhanced following exposure to UVB, suggesting great therapeutic potential for this compound.Conclusion Taken together, our study suggests that A431 respond differently to UVB than normal HaCaT cells, and supports a role for NF‐κB, CDK6, and BCL‐2 in UVB‐induced cell G1‐S phase arrest.Furthermore, salidroside can effectively protect HaCaT from UVB irradiation.
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Abstract: Objective To examine UVB‐induced responses in normal human keratinocytes (HaCaT) and epidermoid carcinoma cells (A431) at the cellular and molecular level, and investigated the protective effect of salidroside.Methods Cells irradiated by UVB at various dosage and their viability was assessed by MTT assays, cell cycle was analysed by flow cytometry. The expression of NF‐κB, BCL‐2, and CDK6 after 50 J/m2 UVB irradiation were detected by RT‐PCR and western blotting.Results Our results confirmed greater tolerance of A341 cells to UVB‐induced damage such as cell viability and cell cycle arrest, which was accompanied by differential expression changes in NF‐κB,BCL‐2, and CDK6. UVB exposure resulted in HaCaT cells undergoing G1‐S phase arrest. When treated with salidroside, HaCaT survival was significantly enhanced following exposure to UVB, suggesting great therapeutic potential for this compound.Conclusion Taken together, our study suggests that A431 respond differently to UVB than normal HaCaT cells, and supports a role for NF‐κB, CDK6, and BCL‐2 in UVB‐induced cell G1‐S phase arrest.Furthermore, salidroside can effectively protect HaCaT from UVB irradiation.
- UVB /
- G1‐S phase arrest /
- HaCaT /
- A431 /
- NF‐κB /
- BCL‐2 /
- CDK6 /
- Salidroside

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Differential Responses to UVB Irradiation in Human Keratinocytes and Epidermoid Carcinoma Cells

doi: 10.3967/0895-3988.2012.05.013

刊出日期: 2012-10-20

关键词:

UVB /

G1‐S phase arrest /

HaCaT /

A431 /

NF‐κB /

BCL‐2 /

CDK6 /

Salidroside

摘要: Objective To examine UVB‐induced responses in normal human keratinocytes (HaCaT) and epidermoid carcinoma cells (A431) at the cellular and molecular level, and investigated the protective effect of salidroside.Methods Cells irradiated by UVB at various dosage and their viability was assessed by MTT assays, cell cycle was analysed by flow cytometry. The expression of NF‐κB, BCL‐2, and CDK6 after 50 J/m2 UVB irradiation were detected by RT‐PCR and western blotting.Results Our results confirmed greater tolerance of A341 cells to UVB‐induced damage such as cell viability and cell cycle arrest, which was accompanied by differential expression changes in NF‐κB,BCL‐2, and CDK6. UVB exposure resulted in HaCaT cells undergoing G1‐S phase arrest. When treated with salidroside, HaCaT survival was significantly enhanced following exposure to UVB, suggesting great therapeutic potential for this compound.Conclusion Taken together, our study suggests that A431 respond differently to UVB than normal HaCaT cells, and supports a role for NF‐κB, CDK6, and BCL‐2 in UVB‐induced cell G1‐S phase arrest.Furthermore, salidroside can effectively protect HaCaT from UVB irradiation.

English Abstract

Differential Responses to UVB Irradiation in Human Keratinocytes and Epidermoid Carcinoma Cells

Department of Radiation Medicine,School of Public Health and Tropical Medicine,Southern Medical University,Guangzhou 510515,Guangdong,China

Publish Date: 2012-10-20

Keywords:

Abstract: Objective To examine UVB‐induced responses in normal human keratinocytes (HaCaT) and epidermoid carcinoma cells (A431) at the cellular and molecular level, and investigated the protective effect of salidroside.Methods Cells irradiated by UVB at various dosage and their viability was assessed by MTT assays, cell cycle was analysed by flow cytometry. The expression of NF‐κB, BCL‐2, and CDK6 after 50 J/m2 UVB irradiation were detected by RT‐PCR and western blotting.Results Our results confirmed greater tolerance of A341 cells to UVB‐induced damage such as cell viability and cell cycle arrest, which was accompanied by differential expression changes in NF‐κB,BCL‐2, and CDK6. UVB exposure resulted in HaCaT cells undergoing G1‐S phase arrest. When treated with salidroside, HaCaT survival was significantly enhanced following exposure to UVB, suggesting great therapeutic potential for this compound.Conclusion Taken together, our study suggests that A431 respond differently to UVB than normal HaCaT cells, and supports a role for NF‐κB, CDK6, and BCL‐2 in UVB‐induced cell G1‐S phase arrest.Furthermore, salidroside can effectively protect HaCaT from UVB irradiation.

Citation:

ZHOU Mei Juan, ZHENG Li, GUO Ling, LIU Wei Ling, LV Chao, JIANG Li Hong, OU Cheng Shan, DING Zhen Hua. Differential Responses to UVB Irradiation in Human Keratinocytes and Epidermoid Carcinoma Cells[J]. Biomedical and Environmental Sciences, 2012, 25(5): 583-589. doi: 10.3967/0895-3988.2012.05.013

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doi: 10.3967/0895-3988.2012.05.013

Differential Responses to UVB Irradiation in Human Keratinocytes and Epidermoid Carcinoma Cells

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Differential Responses to UVB Irradiation in Human Keratinocytes and Epidermoid Carcinoma Cells

doi: 10.3967/0895-3988.2012.05.013

English Abstract

Differential Responses to UVB Irradiation in Human Keratinocytes and Epidermoid Carcinoma Cells

Department of Radiation Medicine,School of Public Health and Tropical Medicine,Southern Medical University,Guangzhou 510515,Guangdong,China

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