WU Jian Jun; YANG Ti; LI Xun; XIA Yuan; ZHAO Yao; ZOU Fei; JIANG Yi Guo

doi:10.3967/bes2014.011

doi: 10.3967/bes2014.011

基金项目: National Key Basic Research Program of China(2012CB525004 to J.Y.)%National Natural Science Foundation of China(30972443 to J.Y.)%Research Fund for Doctoral Program of Higher Education of China(20114423110002 to J.Y.)%Key Program of Guangdong Natural Science Foundation (9251018201000004 to J.Y.). University Talent Program of Guangdong(2010-79 to J.Y.)%University Talent Program of Guangzhou(10A003D to J.Y.)%Science and Technology Project of Guangzhou(2010Y1-C441 to J.Y.)%Youth Foundation of China State Key Laboratory of Respiratory Disease(201105 to W.J.)%Medical Scientific Research Foundation of Guangdong Province(A2011234 to W.J.)

4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone Induces Circulating MicroRNA Deregulation in Early Lung Carcinogenesis

School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, Guangdong, China
Institute for Chemical Carcinogenesis, State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510182, Guangdong, China
School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, Guangdong, China

Funds: National Key Basic Research Program of China(2012CB525004 to J.Y.)%National Natural Science Foundation of China(30972443 to J.Y.)%Research Fund for Doctoral Program of Higher Education of China(20114423110002 to J.Y.)%Key Program of Guangdong Natural Science Foundation (9251018201000004 to J.Y.). University Talent Program of Guangdong(2010-79 to J.Y.)%University Talent Program of Guangzhou(10A003D to J.Y.)%Science and Technology Project of Guangzhou(2010Y1-C441 to J.Y.)%Youth Foundation of China State Key Laboratory of Respiratory Disease(201105 to W.J.)%Medical Scientific Research Foundation of Guangdong Province(A2011234 to W.J.)

摘要: Objective To study the alteration of circulating microRNAs in 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced early stage lung carcinogenesis.
Methods A lung cancer model of male F344 rats was induced with systemic NNK and levels of 8 lung cancer-associated miRNAs in whole blood and serum of rats were measured by quantitative RT-PCR of each at weeks 1, 5, 10, and 20 following NNK treatment.
Results No lung cancer was detected in control group and NNK treatment group at week 20 following NNK treatment. The levels of some circulating miRNAs were significantly higher in NNK treatment group than in control group. The miR-210 was down-regulated and the miR-206 was up-regulated in NNK treatment group. The expression level of circulating miRNAs changed from week 1 to week 20 following NNK treatment.
Conclusion The expression level of circulating miRNAs is related to NNK-induced early stage lung carcinogenesis in rats and can therefore serve as its potential indicator.
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Abstract: Objective To study the alteration of circulating microRNAs in 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced early stage lung carcinogenesis.
Methods A lung cancer model of male F344 rats was induced with systemic NNK and levels of 8 lung cancer-associated miRNAs in whole blood and serum of rats were measured by quantitative RT-PCR of each at weeks 1, 5, 10, and 20 following NNK treatment.
Results No lung cancer was detected in control group and NNK treatment group at week 20 following NNK treatment. The levels of some circulating miRNAs were significantly higher in NNK treatment group than in control group. The miR-210 was down-regulated and the miR-206 was up-regulated in NNK treatment group. The expression level of circulating miRNAs changed from week 1 to week 20 following NNK treatment.
Conclusion The expression level of circulating miRNAs is related to NNK-induced early stage lung carcinogenesis in rats and can therefore serve as its potential indicator.
- NNK /
- Circulating microRNA /
- Early stage /
- Lung carcinogenesis

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doi: 10.3967/bes2014.011

4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone Induces Circulating MicroRNA Deregulation in Early Lung Carcinogenesis

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4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone Induces Circulating MicroRNA Deregulation in Early Lung Carcinogenesis

doi: 10.3967/bes2014.011

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4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone Induces Circulating MicroRNA Deregulation in Early Lung Carcinogenesis

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doi: 10.3967/bes2014.011

4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone Induces Circulating MicroRNA Deregulation in Early Lung Carcinogenesis

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4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone Induces Circulating MicroRNA Deregulation in Early Lung Carcinogenesis

doi: 10.3967/bes2014.011

English Abstract

4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone Induces Circulating MicroRNA Deregulation in Early Lung Carcinogenesis

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目录