ZHU WuYang; TAO Xiao Yan; LYU Xin Jun; YU Peng Cheng; LU Zhuo Zhuang

doi:10.3967/bes2016.028

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

ZHU WuYang, TAO Xiao Yan, LYU Xin Jun, YU Peng Cheng, LU Zhuo Zhuang

文章导航 > Biomedical and Environmental Sciences > 2016 > 29(3): 224-228

ZHU WuYang, TAO Xiao Yan, LYU Xin Jun, YU Peng Cheng, LU Zhuo Zhuang. Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line[J]. Biomedical and Environmental Sciences, 2016, 29(3): 224-228. doi: 10.3967/bes2016.028

Citation:

ZHU WuYang, TAO Xiao Yan, LYU Xin Jun, YU Peng Cheng, LU Zhuo Zhuang. Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line[J]. Biomedical and Environmental Sciences, 2016, 29(3): 224-228. doi: 10.3967/bes2016.028

doi: 10.3967/bes2016.028

基金项目: China Mega-Project for Infectious Disease(2014ZX10004002-004-001)%National Natural Science Foundation of China(31500152)%andNational Key Technology R&D Program(2014BAI13B04)

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control andPrevention, Beijing 102206, China
State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Funds: China Mega-Project for Infectious Disease(2014ZX10004002-004-001)%National Natural Science Foundation of China(31500152)%andNational Key Technology R&D Program(2014BAI13B04)

摘要: The nonstructural protein 1 (NS1) of influenza A virus, which is absent from the viral particle, but highly expressed in infected cells, strongly antagonizes the interferon (IFN)-mediated antiviral response. We engineered an NS1-expressing 293 (293-NS1) cell line with no response to IFN stimulation. Compared with the parental 293 cells, the IFN-nonresponsive 293-NS1 cells improved the growth capacity of various viruses, but the introduction of NS1 barely enhanced the propagation of Tahyna virus, a negative-strand RNA virus. In particular, fastidious enteric adenovirus that replicates poorly in 293 cells may grow more efficiently in 293-NS1 cells; thus, IFN-nonresponsive 293-NS1 cells might be of great value in diagnostic laboratories for the cultivation and isolation of human enteric adenoviruses.
- /
- /
- /
- /
- Cell Line /
- nonstructural protein /
- enteric adenovirus /
- influenza A virus /
- the growth

[1]	. Growth Standard for Newborns by Gestational Age . Biomedical and Environmental Sciences, 2023, 36(7): 665-665. doi: 10.3967/bes2023.097
[2]	SUN Qiang, LEI Na, LU Jian, GAO Rong Bao, LI Zi, LIU Li Qi, SUN Ying, GUO Jun Feng, WANG Da Yan, SHU Yue Long. Interferon-induced Transmembrane Protein 3 Prevents Acute Influenza Pathogenesis in Mice . Biomedical and Environmental Sciences, 2020, 33(5): 295-305. doi: 10.3967/bes2020.041
[3]	Guesmi Fatma, Saidi Issam, Soussi Rawya, Hfaiedh Najla, Landoulsi Ahmed. Antioxidant Potential of Four Species of Natural Product and Therapeutic Strategies for Cancer through Suppression of Viability in the Human Multiple Myeloma Cell Line U266 . Biomedical and Environmental Sciences, 2019, 32(1): 22-33. doi: 10.3967/bes2019.003
[4]	ZHU Yin Chuan, ZHANG Bin, SUN Zeng Hui, WANG Xi Jing, FAN Xiao Hui, GAO Ling Xi, LIANG Ying, CHEN Xiao Yan, ZHANG Zeng Feng. Replication and Pathology of Duck Influenza Virus Subtype H9N2 in Chukar . Biomedical and Environmental Sciences, 2018, 31(4): 306-310. doi: 10.3967/bes2018.039
[5]	ZOU Xiao Hui, CHEN Wen Bing, ZHAO Xiang, ZHU Wen Fei, YANG Lei, WANG Da Yan, SHU Yue Long. Evaluation ofA Single-reaction Method for Whole Genome Sequencing of Influenza A Virus using Next Generation Sequencing . Biomedical and Environmental Sciences, 2016, 29(1): 41-46. doi: 10.3967/bes2016.004
[6]	YAO Yi, XU Cui Ling, SHI Jing Hong, ZHU Yun, LI Yun Fei, BAI Tian, LI Fang Cai, CAI Tao, YUAN Fan, CHEN Tao, YANG Hao, LI Wen Chao, ZHANG Heng Jiao, ZHANG Hong, SHU Yue Long. Phylogenetic and Molecular Analysis of an H7N7 Avian Influenza Virus Isolated in East Dongting Lake in 2012 . Biomedical and Environmental Sciences, 2015, 28(7): 518-526. doi: 10.3967/bes2015.074
[7]	LI Xiao Dan, ZOU Shu Mei, ZHANG Ye, BAI Tian, GAO Rong Bao, ZHANG Xin, WU Jie, SHU Yue Long. A Novel Reassortant H2N3 Influenza Virus Lsolated from China . Biomedical and Environmental Sciences, 2014, 27(4): 240-249. doi: 10.3967/bes2014.047
[8]	ZHAO Ya Ning, LI Qiang. Adenovirus Mediated BIMS Transfer Induces Growth Supression and Apoptosis in Raji Lymphoma Cells . Biomedical and Environmental Sciences, 2014, 27(9): 655-664. doi: 10.3967/bes2014.101
[9]	JI Ya Wei, KONG Yan, ZHAO Ya Hong, WANG Ya Ling, ZHAO Jing, ZHANG Lu Zhong, YANG Yu Min. Controlling the Release of bFGF from Silk Fibroin Membrane . Biomedical and Environmental Sciences, 2014, 27(12): 973-977. doi: 10.3967/bes2014.139
[10]	CHANG Xin Yao, JIANG Jin Qing. Editor Screening Testosterone Residue in the Edible Tissues of Bovine with a Polyclonal Antibody Based Heterologous Immunoassay . Biomedical and Environmental Sciences, 2013, 26(5): 390-393. doi: 10.3967/0895-3988.2013.05.008
[11]	ZHANG Quan, YUAN Wei Feng, ZHAI Guo Qin, ZHU Shan Yuan, XUE Zheng Feng, ZHU Hong Fei, XU Xiang Ming. Inactivated Sendai Virus Suppresses Murine Melanoma Growth by Inducing Host Immune Responses and Down-regulating β-catenin Expression . Biomedical and Environmental Sciences, 2012, 25(5): 509-516. doi: 10.3967/0895-3988.2012.05.003
[12]	LIU LiQi, Li Zi, ZHOU JianFang, ZHU Yun, DONG Jie, ZHAO Xiang, GUO JunFeng, SHU YueLong. Pathogenesis and Immunogenicity of an Avian H9N2 Influenza Virus Isolated from Human . Biomedical and Environmental Sciences, 2011, 24(5): 530-536. doi: 10.3967/0895-3988.2011.05.012
[13]	YAN ShaoMin, WU Guang. Possible Impact of Global Warming on the Evolution of Hemagglutinins from Influenza A Viruses . Biomedical and Environmental Sciences, 2011, 24(1): 62-67. doi: 10.3967/0895-3988.2011.01.008
[14]	XIAO-XING YANG, NING DU, JIAN-FANG ZHOU, ZI LI, MIN WANG, JUN-FENG GUO, DA-YAN WANG, YUE-LONG SHU. Gene Expression Profiles Comparison between 2009 Pandemic and Seasonal H1N1 Influenza Viruses in A549 Cells . Biomedical and Environmental Sciences, 2010, 23(4): 259-266.
[15]	QI-CHUN WEI, LI SHEN, SHU ZHENG, YONG-LIANG ZHU. Isolation and Characterization of Radiation-resistant Lung Cancer D6-R Cell Line . Biomedical and Environmental Sciences, 2008, 21(4): 339-344.
[16]	HAO ZHANG, NA LI, YUE CHEN, LING-YUN HUANG, YI-CHING WANG, GANG FANG, DA-CHENG HE, XUE-YUAN XIAO. Protein Profile of Human Lung Squamous Carcinoma Cell Line NCI-H226 . Biomedical and Environmental Sciences, 2007, 20(1): 24-32.
[17]	Jun-Wen Li, XIU-QUAN SHI, FU-HUAN CHAO, Xin-Wei Wang, Jin-Lai Zheng, NONG Song. A Study on Detecting and Identifying Enteric Pathogens With PCR . Biomedical and Environmental Sciences, 2004, 17(1): 109-120.
[18]	FU YI-CAI, JIN XI-PENG, WEI SHAO-MIN. The Effects on Cell Growth of Tea Polyphenols Acting as a Strong Anti-peroxidatant and an Inhibitor of Apoptosis in Primary Cultured Rat Skin Cells . Biomedical and Environmental Sciences, 2000, 13(3): 170-179.
[19]	R.VIJAYARAGHAVAN, MANINDER SINGH, P.V.L.RAO, BHATTACHARYA, PRAVIN KUMAR, K.SUGENDRAN, OM KUMAR, S.C.PANT, RAM SINGH. Subacute (90 Days) Oral Toxicity Studies of Kombucha Tea . Biomedical and Environmental Sciences, 2000, 13(4): 293-299.
[20]	HE TAo, Huang Cheng-yu, CHEN HAl, HOU YUN-HUA. Effects of Spinach Powder Fat-Soluble Extract on Proliferation of Human Gastric Adenocarcinoma Cells . Biomedical and Environmental Sciences, 1999, 12(4): 247-252.

点击查看大图

计量

文章访问数: 879
HTML全文浏览量: 225
PDF下载量: 52
被引次数: 0

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

doi: 10.3967/bes2016.028

基金项目: China Mega-Project for Infectious Disease(2014ZX10004002-004-001)%National Natural Science Foundation of China(31500152)%andNational Key Technology R&D Program(2014BAI13B04)

刊出日期: 2016-03-20

关键词:

Cell Line /

nonstructural protein /

enteric adenovirus /

influenza A virus /

the growth

摘要: The nonstructural protein 1 (NS1) of influenza A virus, which is absent from the viral particle, but highly expressed in infected cells, strongly antagonizes the interferon (IFN)-mediated antiviral response. We engineered an NS1-expressing 293 (293-NS1) cell line with no response to IFN stimulation. Compared with the parental 293 cells, the IFN-nonresponsive 293-NS1 cells improved the growth capacity of various viruses, but the introduction of NS1 barely enhanced the propagation of Tahyna virus, a negative-strand RNA virus. In particular, fastidious enteric adenovirus that replicates poorly in 293 cells may grow more efficiently in 293-NS1 cells; thus, IFN-nonresponsive 293-NS1 cells might be of great value in diagnostic laboratories for the cultivation and isolation of human enteric adenoviruses.

English Abstract

Citation:

全文HTML

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

Biomedical and Environmental Sciences ISSN 0895-3988 CN 11-2816 / Q

Publish Under the Auspices of Chinese Center for Disease Control and Prevention

Distributed by Chinese Center for Disease Control and Prevention

Quick Links

Supported by Beijing Renhe Information Technology Co. Ltd E-mail: info@rhhz.net

doi: 10.3967/bes2016.028

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

计量

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

doi: 10.3967/bes2016.028

English Abstract

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control andPrevention, Beijing 102206, China

State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China

全文HTML

目录

doi: 10.3967/bes2016.028

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

计量

出版历程

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

doi: 10.3967/bes2016.028

English Abstract

Enhancement of Virus Replication in An Influenza AVirus NS1-Expresssing 293 Cell Line

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control andPrevention, Beijing 102206, China State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China

全文HTML

目录

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control andPrevention, Beijing 102206, China

State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China