LI Xiao Dan; ZOU Shu Mei; ZHANG Ye; BAI Tian; GAO Rong Bao; ZHANG Xin; WU Jie; SHU Yue Long

doi:10.3967/bes2014.047

A Novel Reassortant H2N3 Influenza Virus Lsolated from China

LI Xiao Dan, ZOU Shu Mei, ZHANG Ye, BAI Tian, GAO Rong Bao, ZHANG Xin, WU Jie, SHU Yue Long

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(4): 240-249

LI Xiao Dan, ZOU Shu Mei, ZHANG Ye, BAI Tian, GAO Rong Bao, ZHANG Xin, WU Jie, SHU Yue Long. A Novel Reassortant H2N3 Influenza Virus Lsolated from China[J]. Biomedical and Environmental Sciences, 2014, 27(4): 240-249. doi: 10.3967/bes2014.047

Citation:

LI Xiao Dan, ZOU Shu Mei, ZHANG Ye, BAI Tian, GAO Rong Bao, ZHANG Xin, WU Jie, SHU Yue Long. A Novel Reassortant H2N3 Influenza Virus Lsolated from China[J]. Biomedical and Environmental Sciences, 2014, 27(4): 240-249. doi: 10.3967/bes2014.047

doi: 10.3967/bes2014.047

基金项目: This research is supported by a National Basic Research grant (973)(2011CB504704)

A Novel Reassortant H2N3 Influenza Virus Lsolated from China

Chinese National Influenza Center, Nationallnstitute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory of Surveillance and Early warning on Infectious Disease, Key Laboratory for Medical Virology, National Health and family planning Commission, Beijing 102206, China
Center, for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, Guangdong, China

Funds: This research is supported by a National Basic Research grant (973)(2011CB504704)

摘要: Objective To analyze the genetic composition of a novel H2N3 virus isolate identified from a duck cage swab in a live poultry market (LPM) in 2009 in Guangdong province of China.
Methods PCR-positive specimens were inoculated into embryonated chicken eggs and subtyped by conventional RT-PCR. All segments of the virus A/environment/Guangdong/2/2009 were sequenced, and phylogenetic trees were constructed and analyzed.
Results The genes of this virus belong to Eurasian-lineage avian viruses. The virus is a reassortant with the HA gene from an H2N2 virus and the NA gene from an H5N3 virus. The PB1, PB2, and NP genes were from an H4N6 virus, the PA was from an H3N8 virus, the M gene was from an H1N3 virus, and the NS gene was from an H10N6 virus.
Conclusion market. Its A novel avian-origin reassortant H2N3 influenza virus was detected in a live poultry potential impacts and evolution should be closely monitored.
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- /
Abstract: Objective To analyze the genetic composition of a novel H2N3 virus isolate identified from a duck cage swab in a live poultry market (LPM) in 2009 in Guangdong province of China.
Methods PCR-positive specimens were inoculated into embryonated chicken eggs and subtyped by conventional RT-PCR. All segments of the virus A/environment/Guangdong/2/2009 were sequenced, and phylogenetic trees were constructed and analyzed.
Results The genes of this virus belong to Eurasian-lineage avian viruses. The virus is a reassortant with the HA gene from an H2N2 virus and the NA gene from an H5N3 virus. The PB1, PB2, and NP genes were from an H4N6 virus, the PA was from an H3N8 virus, the M gene was from an H1N3 virus, and the NS gene was from an H10N6 virus.
Conclusion market. Its A novel avian-origin reassortant H2N3 influenza virus was detected in a live poultry potential impacts and evolution should be closely monitored.
- Avian influenza /
- Gene analysis /
- H2N3

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A Novel Reassortant H2N3 Influenza Virus Lsolated from China

doi: 10.3967/bes2014.047

基金项目: This research is supported by a National Basic Research grant (973)(2011CB504704)

刊出日期: 2014-04-20

关键词:

Avian influenza /

Gene analysis /

H2N3

摘要: Objective To analyze the genetic composition of a novel H2N3 virus isolate identified from a duck cage swab in a live poultry market (LPM) in 2009 in Guangdong province of China.
Methods PCR-positive specimens were inoculated into embryonated chicken eggs and subtyped by conventional RT-PCR. All segments of the virus A/environment/Guangdong/2/2009 were sequenced, and phylogenetic trees were constructed and analyzed.
Results The genes of this virus belong to Eurasian-lineage avian viruses. The virus is a reassortant with the HA gene from an H2N2 virus and the NA gene from an H5N3 virus. The PB1, PB2, and NP genes were from an H4N6 virus, the PA was from an H3N8 virus, the M gene was from an H1N3 virus, and the NS gene was from an H10N6 virus.
Conclusion market. Its A novel avian-origin reassortant H2N3 influenza virus was detected in a live poultry potential impacts and evolution should be closely monitored.

English Abstract

A Novel Reassortant H2N3 Influenza Virus Lsolated from China

Chinese National Influenza Center, Nationallnstitute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory of Surveillance and Early warning on Infectious Disease, Key Laboratory for Medical Virology, National Health and family planning Commission, Beijing 102206, China

Center, for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, Guangdong, China

Funds: This research is supported by a National Basic Research grant (973)(2011CB504704)

Publish Date: 2014-04-20

Keywords:

Abstract: Objective To analyze the genetic composition of a novel H2N3 virus isolate identified from a duck cage swab in a live poultry market (LPM) in 2009 in Guangdong province of China.
Methods PCR-positive specimens were inoculated into embryonated chicken eggs and subtyped by conventional RT-PCR. All segments of the virus A/environment/Guangdong/2/2009 were sequenced, and phylogenetic trees were constructed and analyzed.
Results The genes of this virus belong to Eurasian-lineage avian viruses. The virus is a reassortant with the HA gene from an H2N2 virus and the NA gene from an H5N3 virus. The PB1, PB2, and NP genes were from an H4N6 virus, the PA was from an H3N8 virus, the M gene was from an H1N3 virus, and the NS gene was from an H10N6 virus.
Conclusion market. Its A novel avian-origin reassortant H2N3 influenza virus was detected in a live poultry potential impacts and evolution should be closely monitored.

Citation: