DU Ya Rong; PAN Dong; CHEN Ya Xiong; XUE Gang; REN Zhen Xin; LI Xiao Man; ZHANG Shi Chuan; HU Bu Rong

doi:10.3967/bes2015.079

doi: 10.3967/bes2015.079

基金项目: grants from the National Key Scientific Instrument and Equipment Development Project of China(2012YQ03014210)%Major Project Specialized for Infectious Diseases of the Chinese Health and Family Planning Commission(2014ZX10004002-004-002)%National Natural Science Foundation of China [31170803] to BH

Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition

Gansu Key laboratory of Space Radiobiology, Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China
Department of Radiation 0ncology, Sichuan Cancer Hospital, Chengdu 610041, Sichuan, China

Funds: grants from the National Key Scientific Instrument and Equipment Development Project of China(2012YQ03014210)%Major Project Specialized for Infectious Diseases of the Chinese Health and Family Planning Commission(2014ZX10004002-004-002)%National Natural Science Foundation of China [31170803] to BH

摘要: Objective Adipose tissue distributes widely in human body. The irradiation response of the adipose cells in vivo remains to be investigated. In this study we investigated irradiation response of adipose-derived stem cells (ASCs) under three-dimensional culture condition.
Methods ASCs were isolated and cultured in low attachment dishes to form three-dimensional (3D) spheres in vitro. The neuronal differentiation potential and stem-liked characteristics was monitored by using immunofluoresence staining and flow cytometry in monolayer and 3D culture. To investigate the irradiation sensitivity of 3D sphere culture, the fraction of colony survival and micronucleus were detected in monolayer and 3D culture. Soft agar assays were performed for measuring malignant transformation for the irradiated monolayer and 3D culture.
Results The 3D cultured ASCs had higher differentiation potential and an higher stem-like cell percentage. The 3D cultures were more radioresistant after either high linear energy transfer (LET) carbon ion beam or low LET X-ray irradiation compared with the monolayer cell. The ASCs’ potential of cellular transformation was lower after irradiation by soft agar assay.
Conclusion These findings suggest that adipose tissue cell are relatively genomic stable and resistant to genotoxic stress.
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Abstract: Objective Adipose tissue distributes widely in human body. The irradiation response of the adipose cells in vivo remains to be investigated. In this study we investigated irradiation response of adipose-derived stem cells (ASCs) under three-dimensional culture condition.
Methods ASCs were isolated and cultured in low attachment dishes to form three-dimensional (3D) spheres in vitro. The neuronal differentiation potential and stem-liked characteristics was monitored by using immunofluoresence staining and flow cytometry in monolayer and 3D culture. To investigate the irradiation sensitivity of 3D sphere culture, the fraction of colony survival and micronucleus were detected in monolayer and 3D culture. Soft agar assays were performed for measuring malignant transformation for the irradiated monolayer and 3D culture.
Results The 3D cultured ASCs had higher differentiation potential and an higher stem-like cell percentage. The 3D cultures were more radioresistant after either high linear energy transfer (LET) carbon ion beam or low LET X-ray irradiation compared with the monolayer cell. The ASCs’ potential of cellular transformation was lower after irradiation by soft agar assay.
Conclusion These findings suggest that adipose tissue cell are relatively genomic stable and resistant to genotoxic stress.
- Adipose-derived stem cells /
- Three-dimensional cell culture /
- Irradiation response /
- High-and low-LET irradiation

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doi: 10.3967/bes2015.079

Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition

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Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition

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Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition

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doi: 10.3967/bes2015.079

Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition

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Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition

doi: 10.3967/bes2015.079

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Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition

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