KONG YanHua; SHI Qi; ZHANG Ling; ZHANG YuanYuan; GAO TongXin; CHENChen; LIYouLin

doi:10.3967/bes2016.076

doi: 10.3967/bes2016.076

基金项目: This study was supported byYoung Scientists Fund of National Science Foundation of China(81302943, 81302941)%This was a project aimed at promoting the talents of young scientists in 2015(2015-QNYC-A-01)

Structural Modulation of Gut Microbiota in Rats with Allergic Bronchial Asthma Treated with Recuperating Lung Decoction

Beijing University of Chinese Medicine, Beijing 100029, China
Second Department of TCM Pulmonary Disease, The Key Institute of State Administration of Traditional Chinese Medicine pneumonopathy chronic cough and dyspnea, Beijing Key LaboratoryN0. BZ0321, China-Japan Friendship Hospital, Beijing 100029, China
Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100015, China
Nephrology Department, Aviation General Hospital of China Medical University, Beijing 100012, China

Funds: This study was supported byYoung Scientists Fund of National Science Foundation of China(81302943, 81302941)%This was a project aimed at promoting the talents of young scientists in 2015(2015-QNYC-A-01)

摘要: ObjectiveTo investigate whether recuperating lung decoction (RLD) can modulate the composition of gut microbiota in rats during asthma treatment. MethodsFifteen Sprague-Dawley rats were divided randomly and equally into control group, model group, dexamethasone (DEX) group, RLD medium-dose group, and RLD high-dose group. The asthma model was established in all groups, except for the control group. The rats in the DEX and RLD groups were treated orally with DEX and RLD, respectively. The rats in the control and model groups were treated orally with 0.9% saline. The intestinal bacterial communities were compared among groups using 16S rRNA gene amplification and 454 pyrosequencing. ResultsThe microbial flora differed between the control and model groups, but the flora in the RLD groups was similar to that in the control group. No significant differences were observed between the RLD high-dose and medium-dose groups. RLD treatment resulted in an increase in the level beneficial bacteria in the gut, such asLactobacillusandBifidobacteriumspp. ConclusionOral administration of RLD increased the number of intestinal lactic acid-producing bacteria, such as Lactobacillus andBifidobacterium, in asthma model rats.
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Abstract: ObjectiveTo investigate whether recuperating lung decoction (RLD) can modulate the composition of gut microbiota in rats during asthma treatment. MethodsFifteen Sprague-Dawley rats were divided randomly and equally into control group, model group, dexamethasone (DEX) group, RLD medium-dose group, and RLD high-dose group. The asthma model was established in all groups, except for the control group. The rats in the DEX and RLD groups were treated orally with DEX and RLD, respectively. The rats in the control and model groups were treated orally with 0.9% saline. The intestinal bacterial communities were compared among groups using 16S rRNA gene amplification and 454 pyrosequencing. ResultsThe microbial flora differed between the control and model groups, but the flora in the RLD groups was similar to that in the control group. No significant differences were observed between the RLD high-dose and medium-dose groups. RLD treatment resulted in an increase in the level beneficial bacteria in the gut, such asLactobacillusandBifidobacteriumspp. ConclusionOral administration of RLD increased the number of intestinal lactic acid-producing bacteria, such as Lactobacillus andBifidobacterium, in asthma model rats.
- Asthma /
- Gut microbiota /
- Recuperating lung decoction

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doi: 10.3967/bes2016.076

Structural Modulation of Gut Microbiota in Rats with Allergic Bronchial Asthma Treated with Recuperating Lung Decoction

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Structural Modulation of Gut Microbiota in Rats with Allergic Bronchial Asthma Treated with Recuperating Lung Decoction

doi: 10.3967/bes2016.076

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Structural Modulation of Gut Microbiota in Rats with Allergic Bronchial Asthma Treated with Recuperating Lung Decoction

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doi: 10.3967/bes2016.076

Structural Modulation of Gut Microbiota in Rats with Allergic Bronchial Asthma Treated with Recuperating Lung Decoction

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Structural Modulation of Gut Microbiota in Rats with Allergic Bronchial Asthma Treated with Recuperating Lung Decoction

doi: 10.3967/bes2016.076

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Structural Modulation of Gut Microbiota in Rats with Allergic Bronchial Asthma Treated with Recuperating Lung Decoction

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