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This standard specifies the indicators, reference values, and measurement methods for vitamin D deficiency and insufficiency screening in the people.
This standard is applicable to the determination of vitamin D nutritional status in the people.
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The following documents are essential for the application of this document. For the references dated, only their dated versions are applies to this document; for the references undated, their latest versions, including all amendments, are applicable to this document.
GB/T 603 Chemical reagent Preparation of formulations and products used in test methods
GB/T 6682 Analysis of laboratory water specifications and test methods
WS/T 225 Collection and processing of blood samples for clinical chemical testing
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The following terms and definitions apply to this document.
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The main circulating form of vitamin D in the blood, with good stability, is recognized as a reliable indicator for evaluating the nutritional status of vitamin D in the human body. It mainly includes two forms: 25 (OH) D2 and 25 (OH) D3, of which 25 (OH) D3 is the main form of vitamin D present in the blood.
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Vitamin D deficiency can be determined when the content of 25-hydroxyvitamin D in human serum (or plasma) is lower than the reference values for deficiency.
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Vitamin D insufficiency can be determined when the content of 25-hydroxyvitamin D in human serum (or plasma) is lower than the reference values for the normal people, but higher than that for deficiency.
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Determination indicators and reference values for vitamin D nutritional status in the people are shown in Table 1.
Table 1. Determination indicators and reference values for vitamin D nutritional status in the people
Determination indicator Normal Insufficiency Deficiency Content of 25-hydroxyvitamin D
in serum (or plasma)ng/mL nmol/L ng/mL nmol/L ng/mL nmol/L ≥ 20 ≥ 50 ≥ 12 to < 20 ≥ 30 to < 50 < 12 < 30 -
Follow the methods specified in Appendix A.
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Follow the methods specified in Appendix B.
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Follow the methods specified in Appendix C.
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Follow the methods specified in Appendix D.
Method for Vitamin D Deficiency Screening
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Table 1. Determination indicators and reference values for vitamin D nutritional status in the people
Determination indicator Normal Insufficiency Deficiency Content of 25-hydroxyvitamin D
in serum (or plasma)ng/mL nmol/L ng/mL nmol/L ng/mL nmol/L ≥ 20 ≥ 50 ≥ 12 to < 20 ≥ 30 to < 50 < 12 < 30 
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[1] CHEN Xi, HAN Chao, ZHAO Jin Peng, SHEN Shi, WANG Li Yuan, REN Shou, WANG Tong Lei, MA Yan, XU Ze Chao, HUO Jun Sheng. Establishment of an Iron Deficiency Model by Iron Depletion in Pregnant Rats . Biomedical and Environmental Sciences, 2024, 37(2): 210-215. doi: 10.3967/bes2024.022 [2] SUN Hua Lei, ZHAO Tong, ZHANG Dong Dong, FENG Ming Ming, XU Ze, HUANG Hao Yue, ZHANG Luo Ya, LI Wen Jie, LI Xing, DUAN Jia Yu, LI Jia. Interactions of Vitamin D Receptor Polymorphisms with Hypertriglyceridemia and Obesity in Chinese Individuals Susceptible to Hypertension and Diabetes Comorbidity . Biomedical and Environmental Sciences, 2023, 36(2): 196-200. doi: 10.3967/bes2023.022 [3] . Method for Folate Deficiency Screening . Biomedical and Environmental Sciences, 2023, 36(8): 784-784. doi: 10.3967/bes2023.108 [4] FAN Li Jun, GAO Yun Yan, MENG Fan Gang, LIU Chang, LIU Lan Chun, DU Yang, LIU Li Xiang, LI Ming, SU Xiao Hui, LIU Shou Jun, LIU Peng. 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- 网络出版日期: 2023-09-01
- 刊出日期: 2023-09-01
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