Volume 32 Issue 5
May  2019
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JIANG Han Ji, CHEN Na, SHEN Zhi Qiang, YIN Jing, QIU Zhi Gang, MIAO Jing, YANG Zhong Wei, SHI Dan Yang, WANG Hua Ran, WANG Xin Wei, LI Jun Wen, YANG Dong, JIN Min. Inactivation of Poliovirus by Ozone and the Impact of Ozone on the Viral Genome[J]. Biomedical and Environmental Sciences, 2019, 32(5): 324-333. doi: 10.3967/bes2019.044
Citation: JIANG Han Ji, CHEN Na, SHEN Zhi Qiang, YIN Jing, QIU Zhi Gang, MIAO Jing, YANG Zhong Wei, SHI Dan Yang, WANG Hua Ran, WANG Xin Wei, LI Jun Wen, YANG Dong, JIN Min. Inactivation of Poliovirus by Ozone and the Impact of Ozone on the Viral Genome[J]. Biomedical and Environmental Sciences, 2019, 32(5): 324-333. doi: 10.3967/bes2019.044

Inactivation of Poliovirus by Ozone and the Impact of Ozone on the Viral Genome

doi: 10.3967/bes2019.044
Funds:

the National Key R&D Program of China 2018YFC1602500

the National Key R&D Program of China 2017YFC1601200

Tianjin Science and Technology Support Program 16YFZCSF00340

More Information
  • Author Bio:

    JIANG Han Ji, male, born in 1995, Master, majoring in microbiology

    CHEN Na, female, born in 1985, Master, majoring in pharmaceutical engineering

  • Corresponding author: YANG Dong, E-mail:yangd8611@163.com; JIN Min, E-mail:jinminzh@126.com
  • Received Date: 2019-02-15
  • Accepted Date: 2019-05-07
  • Publish Date: 2019-05-01
  • Objective To investigate the mechanisms underlying ozone-induced inactivation of poliovirus type 1 (PV1). Methods We used cell culture, long-overlapping RT-PCR, and spot hybridization assays to verify and accurately locate the sites of action of ozone that cause PV1 inactivation. We also employed recombinant viral genome RNA infection models to confirm our observations. Results Our results indicated that ozone inactivated PV1 primarily by disrupting the 5'-non-coding region (5'-NCR) of the PV1 genome. Further study revealed that ozone specifically damaged the 80-124 nucleotide (nt) region in the 5'-NCR. Recombinant viral genome RNA infection models confirmed that PV1 lacking this region was non-infectious. Conclusion In this study, we not only elucidated the mechanisms by which ozone induces PV1 inactivation but also determined that the 80-124 nt region in the 5'-NCR is targeted by ozone to achieve this inactivation.
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