Inhibitory Effects of Saponins From Anemarrhena asphodeloides Bunge on the Growth of Vascular Smooth Muscle Cells
- Anemarrhena asphodeloides Bunge /
- Saponins /
- Vascular smooth muscle cells /
Abstract: Objective To investigate the effects of saponins from Anemarrhena asphodeloides Bunge (SAaB) (Botanical Name: Anemarrhena Asphodeloidis Rhizoma) on the growth of vascular smooth muscle cells (VSMCs). Methods Cell proliferation was measured by a newly developed cell proliferation reagent, WST-1. Cell apoptosis was assayed by flow cytometry through detecting annexin V. Nitric oxide production was evaluated using confocal laser scanning microscopy with diaminofluorescein diacetate (DAF-2, DA). Cell aldose reductase (AR) activity, as well as the effect of Epalrestat and interleukin-1β were also explored. Results WST assay showed that cell proliferation induced by serum was significantly inhibited by SAaB (P＜0.01). Flow cytometry analysis revealed that SAaB could enhance apoptotic rate of VSMCs (P＜0.01). Nitric oxide production was significantly enhanced after administration of SAaB and interleukin- 1β. Moreover, AR activity of VSMCs was also remarkably inhibited by both SAaB and Epalrestat (P＜0.01). Conclusion SAaB can inhibit proliferation and enhance apoptosis of VSMCs. It may protect vascular cells by inhibiting VSMC proliferation and augmenting apoptotic rate of VSMCs via NO-dependent pathway.
|Citation:||SHANG-ZHI XIAO, MING-EN XU, YA-KUN GE, GUI-FENG XIAO. Inhibitory Effects of Saponins From Anemarrhena asphodeloides Bunge on the Growth of Vascular Smooth Muscle Cells[J]. Biomedical and Environmental Sciences, 2006, 19(3): 185-191.|