YUAN Yan; BIAN Jian Chun; LIU Xue Zhong; ZHANG Ying; SUN Ya; LIU Zong Ping

doi:10.3967/0895-3988.2012.02.008

Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro

YUAN Yan, BIAN Jian Chun, LIU Xue Zhong, ZHANG Ying, SUN Ya, LIU Zong Ping

文章导航 > Biomedical and Environmental Sciences > 2012 > 25(2): 172-181

YUAN Yan, BIAN Jian Chun, LIU Xue Zhong, ZHANG Ying, SUN Ya, LIU Zong Ping. Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro[J]. Biomedical and Environmental Sciences, 2012, 25(2): 172-181. doi: 10.3967/0895-3988.2012.02.008

Citation:

YUAN Yan, BIAN Jian Chun, LIU Xue Zhong, ZHANG Ying, SUN Ya, LIU Zong Ping. Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro[J]. Biomedical and Environmental Sciences, 2012, 25(2): 172-181. doi: 10.3967/0895-3988.2012.02.008

doi: 10.3967/0895-3988.2012.02.008

基金项目: the National Nature Science Foundation of China(30972229%31101866)%Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)

Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro

College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China

Funds: the National Nature Science Foundation of China(30972229%31101866)%Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)

摘要: Objective To investigate the cytotoxic mechanism of cadmium (Cd) on cerebral cortical neurons.Methods The primary cultures of rat cerebral cortical neurons were treated with different concentrations of cadmium acetate (0,5,10,and 20 μmol/L),and then the cell viability,apoptosis,ultrastructure,intracellular [Ca2+],and reactive oxygen species (ROS) levels,mitochondrial membrane potential (Δψ),activities of catalase (CAT) and superoxide dismutase (SOD) were measured.Results A progressive loss in cell viability and an increased number of apoptotic cells were observed.In addition,Cd-induced apoptotic morphological changes in cerebral cortical neurons were also demonstrated by Hoechst 33258 staining.Meanwhile,ultrastructural changes were distortion of mitochondrial cristae and an unusual arrangement.Simultaneously,elevation of intracellular [Ca2+] and ROS levels,depletion of △ψ were revealed in a dose-dependent manner during the exposure.Moreover,CAT and SOD activities in the living cells increased significantly.Conclusion Exposure of cortical neurons to different doses of Cd led to cellular death,mediated by an apoptotic mechanism,and the apoptotic death induced by oxidative stress may be a potential reason.And the disorder of intracellular homeostasis caused by oxidative stress and mitochondrial dysfunction may be a trigger for apoptosis in cortical neurons.
- /
- /
- /
Abstract: Objective To investigate the cytotoxic mechanism of cadmium (Cd) on cerebral cortical neurons.Methods The primary cultures of rat cerebral cortical neurons were treated with different concentrations of cadmium acetate (0,5,10,and 20 μmol/L),and then the cell viability,apoptosis,ultrastructure,intracellular [Ca2+],and reactive oxygen species (ROS) levels,mitochondrial membrane potential (Δψ),activities of catalase (CAT) and superoxide dismutase (SOD) were measured.Results A progressive loss in cell viability and an increased number of apoptotic cells were observed.In addition,Cd-induced apoptotic morphological changes in cerebral cortical neurons were also demonstrated by Hoechst 33258 staining.Meanwhile,ultrastructural changes were distortion of mitochondrial cristae and an unusual arrangement.Simultaneously,elevation of intracellular [Ca2+] and ROS levels,depletion of △ψ were revealed in a dose-dependent manner during the exposure.Moreover,CAT and SOD activities in the living cells increased significantly.Conclusion Exposure of cortical neurons to different doses of Cd led to cellular death,mediated by an apoptotic mechanism,and the apoptotic death induced by oxidative stress may be a potential reason.And the disorder of intracellular homeostasis caused by oxidative stress and mitochondrial dysfunction may be a trigger for apoptosis in cortical neurons.
- Cadmium /
- Apoptosis /
- Oxidative stress /
- Cerebral cortical neurons

[1]	LI Xiao Hong, LIU Yu Mei, SHAN Hui, TAN Jin Feng, ZHOU Jian, SONG Yuan Jin, LI Si Qi, LIU Chen, XU Dong Qun, YU Li, LI Wan Wei. Salidroside Ameliorates Lung Injury Induced by PM_2.5by Regulating SIRT1-PGC-1α in Mice . Biomedical and Environmental Sciences, 2024, 37(4): 367-376. doi: 10.3967/bes2024.041
[2]	LIU Miao Qing, CHEN Hai Qiong, DAI Hai Peng, LI Jing Jing, TIAN Fu Hong, WANG Yi Yan, CHEN Cong De, LI Xiao Heng, LI Jun Wei, LI Zhong Rong, GE Ren Shan. Bis (2-butoxyethyl) Phthalate Delays Puberty Onset by Increasing Oxidative Stress and Apoptosis in Leydig Cells in Rats . Biomedical and Environmental Sciences, 2023, 36(1): 60-75. doi: 10.3967/bes2023.006
[3]	CHEN Zhi Jian, CHEN Jia Xing, WU Li Kang, LI Bi Yun, TIAN Ya Feng, XIAN Min, HUANG Zi Pei, YU Ri An. Induction of Endoplasmic Reticulum Stress by Cadmium and Its Regulation on Nrf2 Signaling Pathway in Kidneys of Rats . Biomedical and Environmental Sciences, 2019, 32(1): 1-10. doi: 10.3967/bes2019.001
[4]	HU Yong Bin, WU Xia, QIN Xiao Feng, WANG Lei, PAN Pin Hua. Role of Endoplasmic Reticulum Stress in Silica-induced Apoptosis in RAW264.7 Cells . Biomedical and Environmental Sciences, 2017, 30(8): 591-600. doi: 10.3967/bes2017.078
[5]	TAO Yong Kang, YU Pu Lin, BAI Yong Ping, YAN Sheng Tao, ZHAO Shui Ping, ZHANG Guo Qiang. Role of PERK/eIF2α/CHOP Endoplasmic Reticulum Stress Pathway in Oxidized Low-density Lipoprotein Mediated Induction of Endothelial Apoptosis . Biomedical and Environmental Sciences, 2016, 29(12): 868-876. doi: 10.3967/bes2016.116
[6]	YUAN Yan, WANG Yi, HU Fei Fei, JIANG Chen Yang, ZHANG Ya Jing, YANG Jin Long, ZHAO Shi Wen, GU Jian Hong, LIU Xue Zhong, BIAN Jian Chun, LIU Zong Ping. Cadmium Activates Reactive Oxygen Species-dependent AKT/mTOR and Mitochondrial Apoptotic Pathways in Neuronal Cells . Biomedical and Environmental Sciences, 2016, 29(2): 117-126. doi: 10.3967/bes2016.013
[7]	YUAN Xuan, ZHANG Bo, GAN Lu, WANG Zhen Hua, YU Ba Cui, LIU Liang Liang, ZHENG Qiu Sheng, WANG Zhi Ping. Involvement of the Mitochondrion-dependent and the Endoplasmic Reticulum Stress-signaling Pathways in Isoliquiritigenin-induced Apoptosis of HeLa Cell . Biomedical and Environmental Sciences, 2013, 26(4): 268-276. doi: 10.3967/0895-3988.2013.04.005
[8]	WANG Lin, LIN Shu Qian, HE Yuan Long, LIU Gang, WANG Zhen Yong. Protective Effects of Quercetin on Cadmium-induced Cytotoxicity in Primary Cultures of Rat Proximal Tubular Cells . Biomedical and Environmental Sciences, 2013, 26(4): 258-267. doi: 10.3967/0895-3988.2013.04.004
[9]	GONG-LIANG LIU, JU-FANG WANG, ZHI-YONG LI, SHI-ZHONG LIANG, XIAO-NING WANG. Immunoassay for Cadmium Detection and Quantification . Biomedical and Environmental Sciences, 2009, 22(3): 188-193.
[10]	RIAN YU, LIN-GFEI HE, XUE-MIN CHEN. Effects of Cadmium on Hepatocellular DNA Damage, Proto-Oncogene Expression and Apoptosis in Rats . Biomedical and Environmental Sciences, 2007, 20(2): 146-153.
[11]	YOU-GEN ZHU, SHU-MEI ZHANG, JI-YUE WANG, WEI-QIANG XIAO, XIN-YU WANG, JUN-FU ZHOU. Overweight and Obesity-Induced Oxidative Stress in Children . Biomedical and Environmental Sciences, 2006, 19(5): 353-359.
[12]	JIAN-GUO LOU, JIE DONG, YI-CHUN ZHENG, SHU-MEI ZHANG, WEI-QIANG XIAO, JUN-FU ZHOU. Increased Oxidative Stress and Damage in Patients With Chronic Bacterial Prostatitis . Biomedical and Environmental Sciences, 2006, 19(6): 481-486.
[13]	RI-AN YU, TAO XIA, AI-GUO WANG, XUE-MIN CHEN. Effects of Selenium and Zinc on Renal Oxidative Stress and Apoptosis Induced by Fluoride in Rats . Biomedical and Environmental Sciences, 2006, 19(6): 439-444.
[14]	JUN-FU ZHOU, XIN-YU WANG, XUE-JUN SHANGGUAN, ZU-MING GAO, SHU-MEI ZHANG, WEI-QIANG XIAO, CHANG-GUI CHEN. Increased Oxidative Stress in Women With Pregnancy-induced Hypertension . Biomedical and Environmental Sciences, 2005, 18(6): 419-426.
[15]	JUN-FU ZHOU, WEN ZHOU, SHU-MEI ZHANG, YAN-ER LUO, Huai-Hong Chen. Oxidative Stress and Free Radical Damage in Patients With Acute Dipterex Poisoning . Biomedical and Environmental Sciences, 2004, 17(2): 223-233.
[16]	YOU-GEN ZHU, JUN-FU ZHOU, WEI-YING SHAN, PEI-SU ZHOU, GUI-ZHONG TONG. Potential Oxidative Stress in the Bodies of Electric Arc Welding Operators:Effect of Photochemical Smog . Biomedical and Environmental Sciences, 2004, 17(4): 381-389.
[17]	JUN-FU ZHOU, WEI-WEI CHEN, GUI-ZHONG TONG. Ozone Emitted During Copying Process -A Potential Cause of Pathological Oxidative Stress and Potential Oxidative Damage in the Bodies of Operators . Biomedical and Environmental Sciences, 2003, 16(2): 95-104.
[18]	ZHOU JUN-FU, CHEN Jia-xi, SHEN Han-chao, CAI DONG. Abnormal Reactions of Free Radicals and Oxidative Damages in the Bodies of Patients With Chronic Glomerulonephritis . Biomedical and Environmental Sciences, 2002, 15(3): 233-244.
[19]	XIE Bo, ZHOU JUN-FU, LU Qun, LI Chong-jian, CHEN PENG. Oxidative Stress in Patients With Acute Coxsackie Virus Myocarditis . Biomedical and Environmental Sciences, 2002, 15(1): 48-57.
[20]	ZHOU JUN-FU, CHEN PENG. Studies on the Oxidative Stress in Alcohol Abusers in China . Biomedical and Environmental Sciences, 2001, 14(3): 180-188.

点击查看大图

计量

文章访问数: 2180
HTML全文浏览量: 667
PDF下载量: 83
被引次数: 0

Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro

doi: 10.3967/0895-3988.2012.02.008

基金项目: the National Nature Science Foundation of China(30972229%31101866)%Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)

刊出日期: 2012-04-20

关键词:

Cadmium /

Apoptosis /

Oxidative stress /

Cerebral cortical neurons

摘要: Objective To investigate the cytotoxic mechanism of cadmium (Cd) on cerebral cortical neurons.Methods The primary cultures of rat cerebral cortical neurons were treated with different concentrations of cadmium acetate (0,5,10,and 20 μmol/L),and then the cell viability,apoptosis,ultrastructure,intracellular [Ca2+],and reactive oxygen species (ROS) levels,mitochondrial membrane potential (Δψ),activities of catalase (CAT) and superoxide dismutase (SOD) were measured.Results A progressive loss in cell viability and an increased number of apoptotic cells were observed.In addition,Cd-induced apoptotic morphological changes in cerebral cortical neurons were also demonstrated by Hoechst 33258 staining.Meanwhile,ultrastructural changes were distortion of mitochondrial cristae and an unusual arrangement.Simultaneously,elevation of intracellular [Ca2+] and ROS levels,depletion of △ψ were revealed in a dose-dependent manner during the exposure.Moreover,CAT and SOD activities in the living cells increased significantly.Conclusion Exposure of cortical neurons to different doses of Cd led to cellular death,mediated by an apoptotic mechanism,and the apoptotic death induced by oxidative stress may be a potential reason.And the disorder of intracellular homeostasis caused by oxidative stress and mitochondrial dysfunction may be a trigger for apoptosis in cortical neurons.

English Abstract

Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro

College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China

Funds: the National Nature Science Foundation of China(30972229%31101866)%Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)

Publish Date: 2012-04-20

Keywords:

Abstract: Objective To investigate the cytotoxic mechanism of cadmium (Cd) on cerebral cortical neurons.Methods The primary cultures of rat cerebral cortical neurons were treated with different concentrations of cadmium acetate (0,5,10,and 20 μmol/L),and then the cell viability,apoptosis,ultrastructure,intracellular [Ca2+],and reactive oxygen species (ROS) levels,mitochondrial membrane potential (Δψ),activities of catalase (CAT) and superoxide dismutase (SOD) were measured.Results A progressive loss in cell viability and an increased number of apoptotic cells were observed.In addition,Cd-induced apoptotic morphological changes in cerebral cortical neurons were also demonstrated by Hoechst 33258 staining.Meanwhile,ultrastructural changes were distortion of mitochondrial cristae and an unusual arrangement.Simultaneously,elevation of intracellular [Ca2+] and ROS levels,depletion of △ψ were revealed in a dose-dependent manner during the exposure.Moreover,CAT and SOD activities in the living cells increased significantly.Conclusion Exposure of cortical neurons to different doses of Cd led to cellular death,mediated by an apoptotic mechanism,and the apoptotic death induced by oxidative stress may be a potential reason.And the disorder of intracellular homeostasis caused by oxidative stress and mitochondrial dysfunction may be a trigger for apoptosis in cortical neurons.

Citation:

YUAN Yan, BIAN Jian Chun, LIU Xue Zhong, ZHANG Ying, SUN Ya, LIU Zong Ping. Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro[J]. Biomedical and Environmental Sciences, 2012, 25(2): 172-181. doi: 10.3967/0895-3988.2012.02.008

全文HTML

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

Biomedical and Environmental Sciences ISSN 0895-3988 CN 11-2816 / Q

Publish Under the Auspices of Chinese Center for Disease Control and Prevention

Distributed by Chinese Center for Disease Control and Prevention

Quick Links

Supported by Beijing Renhe Information Technology Co. Ltd E-mail: info@rhhz.net

doi: 10.3967/0895-3988.2012.02.008

Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro

计量

出版历程

Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro

doi: 10.3967/0895-3988.2012.02.008

English Abstract

Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro

College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China

全文HTML

目录