Evaluation of the Protein Requirement in Chinese Young Adults Using the Indicator Amino Acid Oxidation Technique

LI Min WANG Zhi Ling GOU Ling Yan LI Wei Dong TIAN Yuan HU Yi Chun WANG Rui PIAO Jian Hua YANG Xiao Guang ZHANG Yu Hui

LI Min, WANG Zhi Ling, GOU Ling Yan, LI Wei Dong, TIAN Yuan, HU Yi Chun, WANG Rui, PIAO Jian Hua, YANG Xiao Guang, ZHANG Yu Hui. Evaluation of the Protein Requirement in Chinese Young Adults Using the Indicator Amino Acid Oxidation Technique[J]. Biomedical and Environmental Sciences, 2013, 26(8): 655-662. doi: 10.3967/0895-3988.2013.08.004
Citation: LI Min, WANG Zhi Ling, GOU Ling Yan, LI Wei Dong, TIAN Yuan, HU Yi Chun, WANG Rui, PIAO Jian Hua, YANG Xiao Guang, ZHANG Yu Hui. Evaluation of the Protein Requirement in Chinese Young Adults Using the Indicator Amino Acid Oxidation Technique[J]. Biomedical and Environmental Sciences, 2013, 26(8): 655-662. doi: 10.3967/0895-3988.2013.08.004

doi: 10.3967/0895-3988.2013.08.004
基金项目: the National Natural Science Foundation of China (No.81001247):Dietary reference intakes of protein for Chinese youths

Evaluation of the Protein Requirement in Chinese Young Adults Using the Indicator Amino Acid Oxidation Technique

Funds: the National Natural Science Foundation of China (No.81001247):Dietary reference intakes of protein for Chinese youths
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  • 刊出日期:  2013-08-20

Evaluation of the Protein Requirement in Chinese Young Adults Using the Indicator Amino Acid Oxidation Technique

doi: 10.3967/0895-3988.2013.08.004
    基金项目:  the National Natural Science Foundation of China (No.81001247):Dietary reference intakes of protein for Chinese youths

摘要: Objective To accurately calculate the protein requirements in Chinese young adults using the indicator amino acid oxidation technique. Methods Nine women and ten men received a restricted daily level of protein intake (0.75, 0.82, 0.89, 0.97, and 1.05 g/kg), along with L-[1-13C]-leucine. Subjects’ protein requirement was determined by a biphasic linear regression crossover analysis of F13CO2 data. In doing so, a breakpoint at the minimal rate of appearance of 13CO2 expiration specific to each level of dietary protein was identified. This trial was registered with the Chinese clinical trial registry as ChiCTR-ONC-11001407. Results The Estimated Average Requirement (EAR) and the Recommended Nutrient Intake (RNI) of protein for healthy Chinese young adults were determined to be 0.87 and 0.98 g/(kg·d), respectively, based on the indicator amino acid oxidation technique. Conclusion The EAR and RNI of mixed protein are 5% and 16% that are lower than the current proposed EAR and RNI (0.92 and 1.16 g/(kg·d), respectively), as determined by the nitrogen balance method. The respective EAR and RNI recommendations of 0.87 and 0.98 g/(kg·d) of mixed protein are estimated to be reasonable and suitable for Chinese young adults.

English Abstract

LI Min, WANG Zhi Ling, GOU Ling Yan, LI Wei Dong, TIAN Yuan, HU Yi Chun, WANG Rui, PIAO Jian Hua, YANG Xiao Guang, ZHANG Yu Hui. Evaluation of the Protein Requirement in Chinese Young Adults Using the Indicator Amino Acid Oxidation Technique[J]. Biomedical and Environmental Sciences, 2013, 26(8): 655-662. doi: 10.3967/0895-3988.2013.08.004
Citation: LI Min, WANG Zhi Ling, GOU Ling Yan, LI Wei Dong, TIAN Yuan, HU Yi Chun, WANG Rui, PIAO Jian Hua, YANG Xiao Guang, ZHANG Yu Hui. Evaluation of the Protein Requirement in Chinese Young Adults Using the Indicator Amino Acid Oxidation Technique[J]. Biomedical and Environmental Sciences, 2013, 26(8): 655-662. doi: 10.3967/0895-3988.2013.08.004

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