Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China

LI Ming Hua FU Shi Hong CHEN Wei Xin WANG Huan Yu CAO Yu Xi LIANG Guo Dong

LI Ming Hua, FU Shi Hong, CHEN Wei Xin, WANG Huan Yu, CAO Yu Xi, LIANG Guo Dong. Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China[J]. Biomedical and Environmental Sciences, 2014, 27(4): 231-239. doi: 10.3967/bes2014.046
Citation: LI Ming Hua, FU Shi Hong, CHEN Wei Xin, WANG Huan Yu, CAO Yu Xi, LIANG Guo Dong. Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China[J]. Biomedical and Environmental Sciences, 2014, 27(4): 231-239. doi: 10.3967/bes2014.046

doi: 10.3967/bes2014.046
基金项目: grants from the Ministry of Science and Technology, China(2011CB504702)%National Natural Science Foundation of China(81290342)%Development Grant of State Key Laboratory for Infectious Disease Prevention and Control(2008SKLID105)

Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China

Funds: grants from the Ministry of Science and Technology, China(2011CB504702)%National Natural Science Foundation of China(81290342)%Development Grant of State Key Laboratory for Infectious Disease Prevention and Control(2008SKLID105)
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  • 刊出日期:  2014-04-20

Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China

doi: 10.3967/bes2014.046
    基金项目:  grants from the Ministry of Science and Technology, China(2011CB504702)%National Natural Science Foundation of China(81290342)%Development Grant of State Key Laboratory for Infectious Disease Prevention and Control(2008SKLID105)

摘要: Objective To determine the molecular characterization of full-length genome of Japanese encephalitis virus (JEV) genotype V.
Methods The full-length nucleotide sequences of JEV strains isolated from different locations and sources were used in sequence and phylogenetic analysis.
Results The full-length genome of genotypes V JEV, XZ0934, and Muar strain were composed of 10 983 and 10 988 nucleotides respectively and shared a lower level of identity with JEV genotypes I-IV, ranging from 78.4% (G I, KV1899) to 79.7% (G III, JaGAr01), for the nucleotide sequences, and from 90.0%(G I, KV1899) to 91.8%(G III, JaGAr01) for the amino acid sequences. The open reading frame (ORF) of JEV genotype V spanned nucleotides 96 to 10 397 and encoded 3 433 amino acids. Interestingly, a comparison with JEV genotype I-IV revealed that 3 nucleotides (encoded with a serine residue) were inserted in the NS4A gene of JEV genotype V, and the insertion of nucleotides was also found in downstream of the ORF stop codon in 3’-untranslated region. Moreover, numerous amino acid mutations were observed in 3 functional domains of the E gene of JEV genotype V.
Conclusion The molecular characterization of JEV genotype V is significantly different from that of the known genotypes I-IV. The mutations located in the coding region and the non-coding region may be molecular markers of JEV genotype V and warrant further studies to determine their effects on biology and immunogenicity of genotype V strains.

English Abstract

LI Ming Hua, FU Shi Hong, CHEN Wei Xin, WANG Huan Yu, CAO Yu Xi, LIANG Guo Dong. Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China[J]. Biomedical and Environmental Sciences, 2014, 27(4): 231-239. doi: 10.3967/bes2014.046
Citation: LI Ming Hua, FU Shi Hong, CHEN Wei Xin, WANG Huan Yu, CAO Yu Xi, LIANG Guo Dong. Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China[J]. Biomedical and Environmental Sciences, 2014, 27(4): 231-239. doi: 10.3967/bes2014.046

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