SONG Yang; JIA Zhen Chao; CHEN Jin Yao; HU Jun Xiang; ZHANG Li Shi

doi:10.3967/bes2014.050

Toxic Effects of Atrazine on Reproductive System of Male Rats

SONG Yang, JIA Zhen Chao, CHEN Jin Yao, HU Jun Xiang, ZHANG Li Shi

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(4): 281-288

SONG Yang, JIA Zhen Chao, CHEN Jin Yao, HU Jun Xiang, ZHANG Li Shi. Toxic Effects of Atrazine on Reproductive System of Male Rats[J]. Biomedical and Environmental Sciences, 2014, 27(4): 281-288. doi: 10.3967/bes2014.050

Citation:

SONG Yang, JIA Zhen Chao, CHEN Jin Yao, HU Jun Xiang, ZHANG Li Shi. Toxic Effects of Atrazine on Reproductive System of Male Rats[J]. Biomedical and Environmental Sciences, 2014, 27(4): 281-288. doi: 10.3967/bes2014.050

doi: 10.3967/bes2014.050

基金项目: National Natural Science Foundation of China(81030053)%National High-technology R esearch and Development Program (‘863’ program) of China(2010AA023001)

Toxic Effects of Atrazine on Reproductive System of Male Rats

Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan, China

Funds: National Natural Science Foundation of China(81030053)%National High-technology R esearch and Development Program (‘863’ program) of China(2010AA023001)

摘要: Objective This study was designed to evaluate the toxic effects of Atrazine (ATZ) on the reproductive system of male rats.
Methods Male Sprague-Dawley rats were exposed to ATZ by gavage at dosages of 0, 38.5, 77, and 154 mg/kg bw/day for 30 d. The toxic effects of ATZ to rats were assessed through histopathologcal observation, spermatozoa quality evaluation, testicular marker enzyme indicators, antioxidant capacity and reproductive hormone levels.
Results Significant adverse effects on reproductive system were observed in rats exposed to ATZ at different dosages compared with 0 mg/kg group, including an irregular and disordered arrangement of the seminiferous epithelium in 154 mg/kg group;a decreased spermatozoa number and an increased spermatozoa abnormality rate in 77 and 154 mg/kg groups;decreased levels of acid phosphatase (ACP), alkaline phosphatase (AKP), lactic dehydrogenase (LDH), and succinate dehydrogenase (SDH) with the increasing of ATZ concentration; a decreased level of total antioxidant capacity (TAC) in a dose-dependent manner, and a decreased reduced glutathione (GSH) level and an increased malondialdehyde (MDA) content in 154 mg/kg group;and decreased serum levels of testosterone (T) and inhibin-B (INH-B) and an increased serum level of follicle stimulating hormone (FSH) in 77 and 154 mg/kg groups, and an increased serum level of luteinizing hormone (LH) in 154 mg/kg group.
Conclusion These results suggested that relatively high doses of ATZ could exert reproductive toxicity of male rats.
- /
- /
- /
Abstract: Objective This study was designed to evaluate the toxic effects of Atrazine (ATZ) on the reproductive system of male rats.
Methods Male Sprague-Dawley rats were exposed to ATZ by gavage at dosages of 0, 38.5, 77, and 154 mg/kg bw/day for 30 d. The toxic effects of ATZ to rats were assessed through histopathologcal observation, spermatozoa quality evaluation, testicular marker enzyme indicators, antioxidant capacity and reproductive hormone levels.
Results Significant adverse effects on reproductive system were observed in rats exposed to ATZ at different dosages compared with 0 mg/kg group, including an irregular and disordered arrangement of the seminiferous epithelium in 154 mg/kg group;a decreased spermatozoa number and an increased spermatozoa abnormality rate in 77 and 154 mg/kg groups;decreased levels of acid phosphatase (ACP), alkaline phosphatase (AKP), lactic dehydrogenase (LDH), and succinate dehydrogenase (SDH) with the increasing of ATZ concentration; a decreased level of total antioxidant capacity (TAC) in a dose-dependent manner, and a decreased reduced glutathione (GSH) level and an increased malondialdehyde (MDA) content in 154 mg/kg group;and decreased serum levels of testosterone (T) and inhibin-B (INH-B) and an increased serum level of follicle stimulating hormone (FSH) in 77 and 154 mg/kg groups, and an increased serum level of luteinizing hormone (LH) in 154 mg/kg group.
Conclusion These results suggested that relatively high doses of ATZ could exert reproductive toxicity of male rats.
- Atrazine /
- Reproductive toxicity /
- Oxidative stress /
- Endocrine disrupter

[1]	Jinyu Fang, Huaicun Liu, Yanfei Zhang, Quancheng Cheng, Ziyuan Wang, Xuan Fang, Huiru Ding, Weiguang Zhang, Chunhua Chen. Nogo-A Protein Mediates Oxidative Stress and Synaptic Damage Induced by High-Altitude Hypoxia in the Rat Hippocampus . Biomedical and Environmental Sciences, 2025, 38(1): 79-93. doi: 10.3967/bes2024.112
[2]	WANG Er Hui, YU Zhou, JIA Xu Dong, ZHANG Wen Zhong, andXU Hai Bin. Effects of Parental Dietary Exposure to GMRice TT51 on the Male Reproductive System of Rat Off spring . Biomedical and Environmental Sciences, 2016, 29(4): 267-274. doi: 10.3967/bes2016.034
[3]	LI Shu Gang, DING Yu Song, NIU Qiang, XU Shang Zhi, PANG Li Juan, MA Ru Lin, JING Ming Xia, FENG Gang Ling, LIU Jia Ming, GUO Shu Xia. Grape Seed Proanthocyanidin Extract Alleviates Arsenic-induced Oxidative Reproductive Toxicity in Male Mice . Biomedical and Environmental Sciences, 2015, 28(4): 272-280. doi: 10.3967/bes2015.038
[4]	LIU Qing, GU Jian Hong, YUAN Yan, LIU Xue Zhong, WANG Ya Jun, WANG Han Dong, LIU Zong Ping. Effect of Cadmium on Rat Leydig Cell Testosterone Production and DNA Integrity in vitro . Biomedical and Environmental Sciences, 2013, 26(9): 769-773. doi: 10.3967/0895-3988.2013.09.009
[5]	YUAN Yan, BIAN Jian Chun, LIU Xue Zhong, ZHANG Ying, SUN Ya, LIU Zong Ping. Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro . Biomedical and Environmental Sciences, 2012, 25(2): 172-181. doi: 10.3967/0895-3988.2012.02.008
[6]	DONG CHANG, FAN WANG, YA-SHUANG ZHAO, HONG-ZHI PAN. Evaluation of Oxidative Stress in Colorectal Cancer Patients . Biomedical and Environmental Sciences, 2008, 21(4): 286-289.
[7]	RI-AN YU, TAO XIA, AI-GUO WANG, XUE-MIN CHEN. Effects of Selenium and Zinc on Renal Oxidative Stress and Apoptosis Induced by Fluoride in Rats . Biomedical and Environmental Sciences, 2006, 19(6): 439-444.
[8]	YOU-GEN ZHU, SHU-MEI ZHANG, JI-YUE WANG, WEI-QIANG XIAO, XIN-YU WANG, JUN-FU ZHOU. Overweight and Obesity-Induced Oxidative Stress in Children . Biomedical and Environmental Sciences, 2006, 19(5): 353-359.
[9]	JIAN-GUO LOU, JIE DONG, YI-CHUN ZHENG, SHU-MEI ZHANG, WEI-QIANG XIAO, JUN-FU ZHOU. Increased Oxidative Stress and Damage in Patients With Chronic Bacterial Prostatitis . Biomedical and Environmental Sciences, 2006, 19(6): 481-486.
[10]	JUN-FU ZHOU, XIN-YU WANG, XUE-JUN SHANGGUAN, ZU-MING GAO, SHU-MEI ZHANG, WEI-QIANG XIAO, CHANG-GUI CHEN. Increased Oxidative Stress in Women With Pregnancy-induced Hypertension . Biomedical and Environmental Sciences, 2005, 18(6): 419-426.
[11]	JUN-FU ZHOU, WEN ZHOU, SHU-MEI ZHANG, YAN-ER LUO, Huai-Hong Chen. Oxidative Stress and Free Radical Damage in Patients With Acute Dipterex Poisoning . Biomedical and Environmental Sciences, 2004, 17(2): 223-233.
[12]	YOU-GEN ZHU, JUN-FU ZHOU, WEI-YING SHAN, PEI-SU ZHOU, GUI-ZHONG TONG. Potential Oxidative Stress in the Bodies of Electric Arc Welding Operators:Effect of Photochemical Smog . Biomedical and Environmental Sciences, 2004, 17(4): 381-389.
[13]	P.DIPTI, B.YOGESH, A.K.KAIN, T.PAULINE, B.ANJU, M.SAIRAM, B.Singh, S.S.MONGIA, G.ILAVAZHAGAN DEVENDRA KUMAR, W.SELVAMURTHY. Lead Induced Oxidative Stress: Beneficial Effects of Kombucha Tea . Biomedical and Environmental Sciences, 2003, 16(3): 276-282.
[14]	JUN-FU ZHOU, YE-HUA ZHOU, Liang Zhang, Huai-Hong Chen, DONG CAI. 3,4-Methylenedioxymethamphetamine (MDMA) Abuse Markedly Inhibits Acetylcholinesterase Activity and Induces Severe Oxidative Damage and Liperoxidative Damage . Biomedical and Environmental Sciences, 2003, 16(1): 53-61.
[15]	JUN-FU ZHOU, WEI-WEI CHEN, GUI-ZHONG TONG. Ozone Emitted During Copying Process -A Potential Cause of Pathological Oxidative Stress and Potential Oxidative Damage in the Bodies of Operators . Biomedical and Environmental Sciences, 2003, 16(2): 95-104.
[16]	HONG Zhou, Xiao-jian ZHANG, Zhan-sheng WANG. Research Development of Endocrine Disrupting Chemicals (EDCs) in Water in China . Biomedical and Environmental Sciences, 2003, 16(1): 62-67.
[17]	ZHOU JUN-FU, CHEN Jia-xi, SHEN Han-chao, CAI DONG. Abnormal Reactions of Free Radicals and Oxidative Damages in the Bodies of Patients With Chronic Glomerulonephritis . Biomedical and Environmental Sciences, 2002, 15(3): 233-244.
[18]	XIE Bo, ZHOU JUN-FU, LU Qun, LI Chong-jian, CHEN PENG. Oxidative Stress in Patients With Acute Coxsackie Virus Myocarditis . Biomedical and Environmental Sciences, 2002, 15(1): 48-57.
[19]	ZHOU JUN-FU, CHEN PENG. Studies on the Oxidative Stress in Alcohol Abusers in China . Biomedical and Environmental Sciences, 2001, 14(3): 180-188.
[20]	ZHOU JUN-FU, CHEN PENG, YANG JIN-LU, ZHU YOU-GEN, PENG Cheng-hong, WU Yu-lian. Oxidative Stress before and after Operation in Patients with Chronic Cholecystitis Containing Gallstone . Biomedical and Environmental Sciences, 2000, 13(4): 254-262.

点击查看大图

计量

文章访问数: 2239
HTML全文浏览量: 881
PDF下载量: 156
被引次数: 0

Toxic Effects of Atrazine on Reproductive System of Male Rats

doi: 10.3967/bes2014.050

基金项目: National Natural Science Foundation of China(81030053)%National High-technology R esearch and Development Program (‘863’ program) of China(2010AA023001)

刊出日期: 2014-04-20

关键词:

Atrazine /

Reproductive toxicity /

Oxidative stress /

Endocrine disrupter

摘要: Objective This study was designed to evaluate the toxic effects of Atrazine (ATZ) on the reproductive system of male rats.
Methods Male Sprague-Dawley rats were exposed to ATZ by gavage at dosages of 0, 38.5, 77, and 154 mg/kg bw/day for 30 d. The toxic effects of ATZ to rats were assessed through histopathologcal observation, spermatozoa quality evaluation, testicular marker enzyme indicators, antioxidant capacity and reproductive hormone levels.
Results Significant adverse effects on reproductive system were observed in rats exposed to ATZ at different dosages compared with 0 mg/kg group, including an irregular and disordered arrangement of the seminiferous epithelium in 154 mg/kg group;a decreased spermatozoa number and an increased spermatozoa abnormality rate in 77 and 154 mg/kg groups;decreased levels of acid phosphatase (ACP), alkaline phosphatase (AKP), lactic dehydrogenase (LDH), and succinate dehydrogenase (SDH) with the increasing of ATZ concentration; a decreased level of total antioxidant capacity (TAC) in a dose-dependent manner, and a decreased reduced glutathione (GSH) level and an increased malondialdehyde (MDA) content in 154 mg/kg group;and decreased serum levels of testosterone (T) and inhibin-B (INH-B) and an increased serum level of follicle stimulating hormone (FSH) in 77 and 154 mg/kg groups, and an increased serum level of luteinizing hormone (LH) in 154 mg/kg group.
Conclusion These results suggested that relatively high doses of ATZ could exert reproductive toxicity of male rats.

English Abstract

Toxic Effects of Atrazine on Reproductive System of Male Rats

Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan, China

Funds: National Natural Science Foundation of China(81030053)%National High-technology R esearch and Development Program (‘863’ program) of China(2010AA023001)

Publish Date: 2014-04-20

Keywords:

Abstract: Objective This study was designed to evaluate the toxic effects of Atrazine (ATZ) on the reproductive system of male rats.
Methods Male Sprague-Dawley rats were exposed to ATZ by gavage at dosages of 0, 38.5, 77, and 154 mg/kg bw/day for 30 d. The toxic effects of ATZ to rats were assessed through histopathologcal observation, spermatozoa quality evaluation, testicular marker enzyme indicators, antioxidant capacity and reproductive hormone levels.
Results Significant adverse effects on reproductive system were observed in rats exposed to ATZ at different dosages compared with 0 mg/kg group, including an irregular and disordered arrangement of the seminiferous epithelium in 154 mg/kg group;a decreased spermatozoa number and an increased spermatozoa abnormality rate in 77 and 154 mg/kg groups;decreased levels of acid phosphatase (ACP), alkaline phosphatase (AKP), lactic dehydrogenase (LDH), and succinate dehydrogenase (SDH) with the increasing of ATZ concentration; a decreased level of total antioxidant capacity (TAC) in a dose-dependent manner, and a decreased reduced glutathione (GSH) level and an increased malondialdehyde (MDA) content in 154 mg/kg group;and decreased serum levels of testosterone (T) and inhibin-B (INH-B) and an increased serum level of follicle stimulating hormone (FSH) in 77 and 154 mg/kg groups, and an increased serum level of luteinizing hormone (LH) in 154 mg/kg group.
Conclusion These results suggested that relatively high doses of ATZ could exert reproductive toxicity of male rats.

Citation: