LIU Xiao Tong; MU Xi Yan; WU Xiao Li; MENG Li Xuan; GUAN Wen Bi; MA Yong Qiang; SUN Hua; WANG Cheng Ju; LI Xue Feng

doi:10.3967/bes2014.103

Toxicity of Multi-Walled Carbon Nanotubes, Graphene Oxide, and Reduced Graphene Oxide to Zebrafish Embryos

LIU Xiao Tong, MU Xi Yan, WU Xiao Li, MENG Li Xuan, GUAN Wen Bi, MA Yong Qiang, SUN Hua, WANG Cheng Ju, LI Xue Feng

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(9): 676-683

LIU Xiao Tong, MU Xi Yan, WU Xiao Li, MENG Li Xuan, GUAN Wen Bi, MA Yong Qiang, SUN Hua, WANG Cheng Ju, LI Xue Feng. Toxicity of Multi-Walled Carbon Nanotubes, Graphene Oxide, and Reduced Graphene Oxide to Zebrafish Embryos[J]. Biomedical and Environmental Sciences, 2014, 27(9): 676-683. doi: 10.3967/bes2014.103

Citation:

LIU Xiao Tong, MU Xi Yan, WU Xiao Li, MENG Li Xuan, GUAN Wen Bi, MA Yong Qiang, SUN Hua, WANG Cheng Ju, LI Xue Feng. Toxicity of Multi-Walled Carbon Nanotubes, Graphene Oxide, and Reduced Graphene Oxide to Zebrafish Embryos[J]. Biomedical and Environmental Sciences, 2014, 27(9): 676-683. doi: 10.3967/bes2014.103

doi: 10.3967/bes2014.103

基金项目: the Specialized Research Fund for the Doctoral Program of Higher Education(200800191013)%the Fundamental Research Funds for the Central Universities

Toxicity of Multi-Walled Carbon Nanotubes, Graphene Oxide, and Reduced Graphene Oxide to Zebrafish Embryos

College of Science, China Agricultural University, Beijing 100193, China
College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China

Funds: the Specialized Research Fund for the Doctoral Program of Higher Education(200800191013)%the Fundamental Research Funds for the Central Universities

摘要: Objective This study was aimed to investigate the toxic effects of 3 nanomaterials, i.e. multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO), and reduced graphene oxide (RGO), on zebrafish embryos. Methods The 2-h post-fertilization (hpf) zebrafish embryos were exposed to MWCNTs, GO, and RGO at different concentrations (1, 5, 10, 50, 100 mg/L) for 96 h. Afterwards, the effects of the 3 nanomateria on spontaneous movement, heart rate, hatching rate, length of larvae, mortality, and malformations ls were evaluated. Results Statistical analysis indicated that RGO significantly inhibited the hatching of zebrafish embryos. Furthermore, RGO and MWCNTs decreased the length of the hatched larvae at 96 hpf. No obvious morphological malformation or mortality was observed in the zebrafish embryos after exposure to the three nanomaterials. Conclusion MWCNTs, GO, and RGO were all toxic to zebrafish embryos to influence embryos hatching and larvae length. Although no obvious morphological malformation and mortality were observed in exposed zebrafish embryos, further studies on the toxicity of the three nanomaterials are still needed.
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Abstract: Objective This study was aimed to investigate the toxic effects of 3 nanomaterials, i.e. multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO), and reduced graphene oxide (RGO), on zebrafish embryos. Methods The 2-h post-fertilization (hpf) zebrafish embryos were exposed to MWCNTs, GO, and RGO at different concentrations (1, 5, 10, 50, 100 mg/L) for 96 h. Afterwards, the effects of the 3 nanomateria on spontaneous movement, heart rate, hatching rate, length of larvae, mortality, and malformations ls were evaluated. Results Statistical analysis indicated that RGO significantly inhibited the hatching of zebrafish embryos. Furthermore, RGO and MWCNTs decreased the length of the hatched larvae at 96 hpf. No obvious morphological malformation or mortality was observed in the zebrafish embryos after exposure to the three nanomaterials. Conclusion MWCNTs, GO, and RGO were all toxic to zebrafish embryos to influence embryos hatching and larvae length. Although no obvious morphological malformation and mortality were observed in exposed zebrafish embryos, further studies on the toxicity of the three nanomaterials are still needed.
- Zebrafish Embryos /
- Toxicity /
- Multi-Walled Carbon Nanotubes /
- Graphene Oxide /
- Reduced Graphene Oxide

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Toxicity of Multi-Walled Carbon Nanotubes, Graphene Oxide, and Reduced Graphene Oxide to Zebrafish Embryos

doi: 10.3967/bes2014.103

基金项目: the Specialized Research Fund for the Doctoral Program of Higher Education(200800191013)%the Fundamental Research Funds for the Central Universities

刊出日期: 2014-09-20

关键词:

Zebrafish Embryos /

Toxicity /

Multi-Walled Carbon Nanotubes /

Graphene Oxide /

Reduced Graphene Oxide

摘要: Objective This study was aimed to investigate the toxic effects of 3 nanomaterials, i.e. multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO), and reduced graphene oxide (RGO), on zebrafish embryos. Methods The 2-h post-fertilization (hpf) zebrafish embryos were exposed to MWCNTs, GO, and RGO at different concentrations (1, 5, 10, 50, 100 mg/L) for 96 h. Afterwards, the effects of the 3 nanomateria on spontaneous movement, heart rate, hatching rate, length of larvae, mortality, and malformations ls were evaluated. Results Statistical analysis indicated that RGO significantly inhibited the hatching of zebrafish embryos. Furthermore, RGO and MWCNTs decreased the length of the hatched larvae at 96 hpf. No obvious morphological malformation or mortality was observed in the zebrafish embryos after exposure to the three nanomaterials. Conclusion MWCNTs, GO, and RGO were all toxic to zebrafish embryos to influence embryos hatching and larvae length. Although no obvious morphological malformation and mortality were observed in exposed zebrafish embryos, further studies on the toxicity of the three nanomaterials are still needed.

English Abstract

Toxicity of Multi-Walled Carbon Nanotubes, Graphene Oxide, and Reduced Graphene Oxide to Zebrafish Embryos

College of Science, China Agricultural University, Beijing 100193, China

College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China

Funds: the Specialized Research Fund for the Doctoral Program of Higher Education(200800191013)%the Fundamental Research Funds for the Central Universities

Publish Date: 2014-09-20

Keywords:

Abstract: Objective This study was aimed to investigate the toxic effects of 3 nanomaterials, i.e. multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO), and reduced graphene oxide (RGO), on zebrafish embryos. Methods The 2-h post-fertilization (hpf) zebrafish embryos were exposed to MWCNTs, GO, and RGO at different concentrations (1, 5, 10, 50, 100 mg/L) for 96 h. Afterwards, the effects of the 3 nanomateria on spontaneous movement, heart rate, hatching rate, length of larvae, mortality, and malformations ls were evaluated. Results Statistical analysis indicated that RGO significantly inhibited the hatching of zebrafish embryos. Furthermore, RGO and MWCNTs decreased the length of the hatched larvae at 96 hpf. No obvious morphological malformation or mortality was observed in the zebrafish embryos after exposure to the three nanomaterials. Conclusion MWCNTs, GO, and RGO were all toxic to zebrafish embryos to influence embryos hatching and larvae length. Although no obvious morphological malformation and mortality were observed in exposed zebrafish embryos, further studies on the toxicity of the three nanomaterials are still needed.

Citation:

LIU Xiao Tong, MU Xi Yan, WU Xiao Li, MENG Li Xuan, GUAN Wen Bi, MA Yong Qiang, SUN Hua, WANG Cheng Ju, LI Xue Feng. Toxicity of Multi-Walled Carbon Nanotubes, Graphene Oxide, and Reduced Graphene Oxide to Zebrafish Embryos[J]. Biomedical and Environmental Sciences, 2014, 27(9): 676-683. doi: 10.3967/bes2014.103

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doi: 10.3967/bes2014.103

Toxicity of Multi-Walled Carbon Nanotubes, Graphene Oxide, and Reduced Graphene Oxide to Zebrafish Embryos

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Toxicity of Multi-Walled Carbon Nanotubes, Graphene Oxide, and Reduced Graphene Oxide to Zebrafish Embryos

doi: 10.3967/bes2014.103

English Abstract

Toxicity of Multi-Walled Carbon Nanotubes, Graphene Oxide, and Reduced Graphene Oxide to Zebrafish Embryos

College of Science, China Agricultural University, Beijing 100193, China College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China

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College of Science, China Agricultural University, Beijing 100193, China

College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China