LI WanFang; WEI JinFeng; JIN HongTao; HUANG MingFang; ZHANG JingXuan; LI ChengHe; CHEN ChaoJie; LIU Chang; WANG AiPing

doi:10.3967/0895-3988.2011.04.009

Study of the Toxicity of 1-Bromo-3-Chloro-5,5-Dimethylhydantoin to Zebrafish

LI WanFang, WEI JinFeng, JIN HongTao, HUANG MingFang, ZHANG JingXuan, LI ChengHe, CHEN ChaoJie, LIU Chang, WANG AiPing

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(4): 383-390

LI WanFang, WEI JinFeng, JIN HongTao, HUANG MingFang, ZHANG JingXuan, LI ChengHe, CHEN ChaoJie, LIU Chang, WANG AiPing. Study of the Toxicity of 1-Bromo-3-Chloro-5,5-Dimethylhydantoin to Zebrafish[J]. Biomedical and Environmental Sciences, 2011, 24(4): 383-390. doi: 10.3967/0895-3988.2011.04.009

Citation:

LI WanFang, WEI JinFeng, JIN HongTao, HUANG MingFang, ZHANG JingXuan, LI ChengHe, CHEN ChaoJie, LIU Chang, WANG AiPing. Study of the Toxicity of 1-Bromo-3-Chloro-5,5-Dimethylhydantoin to Zebrafish[J]. Biomedical and Environmental Sciences, 2011, 24(4): 383-390. doi: 10.3967/0895-3988.2011.04.009

doi: 10.3967/0895-3988.2011.04.009

基金项目: the National Science and Technology Major Project "Creation of Major New Drugs"(2008ZX09305-001)

Study of the Toxicity of 1-Bromo-3-Chloro-5,5-Dimethylhydantoin to Zebrafish

Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050 China

Funds: the National Science and Technology Major Project "Creation of Major New Drugs"(2008ZX09305-001)

摘要: Objective 1-Bromo-3-chloro-5,5-dimethylhydantoin (BCDMH) is a solid oxidizing biocide for water disinfection.The objective of this study was to investigate the toxic effect of BCDMH on zebrafish.Methods The developmental toxicity of BCDMH on zebrafish embryos and the dose-effect relationship was determined.The effect of BCDMH exposure on histopathology and tissue antioxidant activity of adult zebrafish were observed over time.Results Exposure to 4 mg/L BCDMH post-fertilization was sufficient to induce a number of developmental malformations,such as edema,axial malformations,and reductions in heart rate and hatching rate.The no observable effects concentration of BCDMH on zebrafish embryo was 0.5 mg/L.After 96 h exposure,the 50％ lethal concentration (95％ confidence interval (CI)) of BCDMH on zebrafish embryo was 8.10 mg/L (6.15-11.16 mg/L).The 50％ inhibitory concentration (95％ CI) of BCDMH on hatching rate was 7.37 mg/L (6.33-8.35 mg/L).Histopathology showed two types of responses induced by BCDMH,defensive and compensatory.The extreme responses were marked hyperplasia of the gill epithelium with lamellar fusion and epidermal peeling.The histopathologic changes in the gills after 10 days exposure were accompanied by significantly higher catalase activity and lipid peroxidation.Conclusion These results have important implications for studies on the toxicity and use of BCDMH and its analogs.
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Abstract: Objective 1-Bromo-3-chloro-5,5-dimethylhydantoin (BCDMH) is a solid oxidizing biocide for water disinfection.The objective of this study was to investigate the toxic effect of BCDMH on zebrafish.Methods The developmental toxicity of BCDMH on zebrafish embryos and the dose-effect relationship was determined.The effect of BCDMH exposure on histopathology and tissue antioxidant activity of adult zebrafish were observed over time.Results Exposure to 4 mg/L BCDMH post-fertilization was sufficient to induce a number of developmental malformations,such as edema,axial malformations,and reductions in heart rate and hatching rate.The no observable effects concentration of BCDMH on zebrafish embryo was 0.5 mg/L.After 96 h exposure,the 50％ lethal concentration (95％ confidence interval (CI)) of BCDMH on zebrafish embryo was 8.10 mg/L (6.15-11.16 mg/L).The 50％ inhibitory concentration (95％ CI) of BCDMH on hatching rate was 7.37 mg/L (6.33-8.35 mg/L).Histopathology showed two types of responses induced by BCDMH,defensive and compensatory.The extreme responses were marked hyperplasia of the gill epithelium with lamellar fusion and epidermal peeling.The histopathologic changes in the gills after 10 days exposure were accompanied by significantly higher catalase activity and lipid peroxidation.Conclusion These results have important implications for studies on the toxicity and use of BCDMH and its analogs.
- Developmental toxicity /
- Histopathological effects /
- 1-Bromo-3-chloro-5,5-dimethylhydantoin(BCDMH)

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Study of the Toxicity of 1-Bromo-3-Chloro-5,5-Dimethylhydantoin to Zebrafish

doi: 10.3967/0895-3988.2011.04.009

基金项目: the National Science and Technology Major Project "Creation of Major New Drugs"(2008ZX09305-001)

刊出日期: 2011-08-20

关键词:

Developmental toxicity /

Histopathological effects /

1-Bromo-3-chloro-5,5-dimethylhydantoin(BCDMH)

摘要: Objective 1-Bromo-3-chloro-5,5-dimethylhydantoin (BCDMH) is a solid oxidizing biocide for water disinfection.The objective of this study was to investigate the toxic effect of BCDMH on zebrafish.Methods The developmental toxicity of BCDMH on zebrafish embryos and the dose-effect relationship was determined.The effect of BCDMH exposure on histopathology and tissue antioxidant activity of adult zebrafish were observed over time.Results Exposure to 4 mg/L BCDMH post-fertilization was sufficient to induce a number of developmental malformations,such as edema,axial malformations,and reductions in heart rate and hatching rate.The no observable effects concentration of BCDMH on zebrafish embryo was 0.5 mg/L.After 96 h exposure,the 50％ lethal concentration (95％ confidence interval (CI)) of BCDMH on zebrafish embryo was 8.10 mg/L (6.15-11.16 mg/L).The 50％ inhibitory concentration (95％ CI) of BCDMH on hatching rate was 7.37 mg/L (6.33-8.35 mg/L).Histopathology showed two types of responses induced by BCDMH,defensive and compensatory.The extreme responses were marked hyperplasia of the gill epithelium with lamellar fusion and epidermal peeling.The histopathologic changes in the gills after 10 days exposure were accompanied by significantly higher catalase activity and lipid peroxidation.Conclusion These results have important implications for studies on the toxicity and use of BCDMH and its analogs.

English Abstract

Study of the Toxicity of 1-Bromo-3-Chloro-5,5-Dimethylhydantoin to Zebrafish

Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050 China

Funds: the National Science and Technology Major Project "Creation of Major New Drugs"(2008ZX09305-001)

Publish Date: 2011-08-20

Keywords:

Developmental toxicity /
Histopathological effects /
1-Bromo-3-chloro-5,5-dimethylhydantoin(BCDMH)

Abstract: Objective 1-Bromo-3-chloro-5,5-dimethylhydantoin (BCDMH) is a solid oxidizing biocide for water disinfection.The objective of this study was to investigate the toxic effect of BCDMH on zebrafish.Methods The developmental toxicity of BCDMH on zebrafish embryos and the dose-effect relationship was determined.The effect of BCDMH exposure on histopathology and tissue antioxidant activity of adult zebrafish were observed over time.Results Exposure to 4 mg/L BCDMH post-fertilization was sufficient to induce a number of developmental malformations,such as edema,axial malformations,and reductions in heart rate and hatching rate.The no observable effects concentration of BCDMH on zebrafish embryo was 0.5 mg/L.After 96 h exposure,the 50％ lethal concentration (95％ confidence interval (CI)) of BCDMH on zebrafish embryo was 8.10 mg/L (6.15-11.16 mg/L).The 50％ inhibitory concentration (95％ CI) of BCDMH on hatching rate was 7.37 mg/L (6.33-8.35 mg/L).Histopathology showed two types of responses induced by BCDMH,defensive and compensatory.The extreme responses were marked hyperplasia of the gill epithelium with lamellar fusion and epidermal peeling.The histopathologic changes in the gills after 10 days exposure were accompanied by significantly higher catalase activity and lipid peroxidation.Conclusion These results have important implications for studies on the toxicity and use of BCDMH and its analogs.

Citation:

LI WanFang, WEI JinFeng, JIN HongTao, HUANG MingFang, ZHANG JingXuan, LI ChengHe, CHEN ChaoJie, LIU Chang, WANG AiPing. Study of the Toxicity of 1-Bromo-3-Chloro-5,5-Dimethylhydantoin to Zebrafish[J]. Biomedical and Environmental Sciences, 2011, 24(4): 383-390. doi: 10.3967/0895-3988.2011.04.009