Carbon Monoxide Releasing Molecule Accelerates Reendothelialization after Carotid Artery Balloon Injury in Rat

HU Qing Song CHEN Yang Xin HUANG Qing Sheng DENG Bing Qing XIE Shuang Lun WANG Jing Feng NIE Ru Qiong

HU Qing Song, CHEN Yang Xin, HUANG Qing Sheng, DENG Bing Qing, XIE Shuang Lun, WANG Jing Feng, NIE Ru Qiong. Carbon Monoxide Releasing Molecule Accelerates Reendothelialization after Carotid Artery Balloon Injury in Rat[J]. Biomedical and Environmental Sciences, 2015, 28(4): 253-262. doi: 10.3967/bes2015.036
Citation: HU Qing Song, CHEN Yang Xin, HUANG Qing Sheng, DENG Bing Qing, XIE Shuang Lun, WANG Jing Feng, NIE Ru Qiong. Carbon Monoxide Releasing Molecule Accelerates Reendothelialization after Carotid Artery Balloon Injury in Rat[J]. Biomedical and Environmental Sciences, 2015, 28(4): 253-262. doi: 10.3967/bes2015.036

doi: 10.3967/bes2015.036
基金项目: This study was supported by grants from the National Natural Science Foundation of China(30770899 and 81370309)%Natural Science Foundation of Guangdong Province(S2012010009659)

Carbon Monoxide Releasing Molecule Accelerates Reendothelialization after Carotid Artery Balloon Injury in Rat

Funds: This study was supported by grants from the National Natural Science Foundation of China(30770899 and 81370309)%Natural Science Foundation of Guangdong Province(S2012010009659)
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  • 刊出日期:  2015-04-20

Carbon Monoxide Releasing Molecule Accelerates Reendothelialization after Carotid Artery Balloon Injury in Rat

doi: 10.3967/bes2015.036
    基金项目:  This study was supported by grants from the National Natural Science Foundation of China(30770899 and 81370309)%Natural Science Foundation of Guangdong Province(S2012010009659)

摘要: Objective This study was aimed to investigate the effects of carbon monoxide releasing molecule (CORM-2), a novel carbon monoxide carrier, on the reendothelialization of carotid artery in rat endothelial denudation model.
Methods Male rats subjected to carotid artery balloon injury were treated with CORM-2, inactive CORM-2 (iCORM-2) or dimethyl sulfoxide (DMSO). The reendothelialization capacity was evaluated by Evans Blue dye and the immunostaining with anti-CD31 antibody. The number of circulating endothelial progenitor cells (EPCs) was detected by flow cytometry. The proliferation, migration, and adhesion of human umbilical vein endothelial cells (HUVECs) were assessed by using [3H]thymidine, Boyden chamber and human fibronectin respectively. The expressions of protein were detected by using western blot analysis.
Results CORM-2 remarkably accelerated the re-endothelialization 5 d later and inhibited neointima formation 28 d later. In addition, the number of peripheral EPCs significantly increased in CORM-2-treated rats than that in iCORM-2 or DMSO-treated rats after 5 d later. In vitro experiments, CORM-2 significantly enhanced the proliferation, migration and adhesion of HUVECs. The levels of Akt, eNOS phosphorylation, and NO generation in HUVECs were also much higher in CORM-2 treated group. Blocking of PI3K/Akt/eNOS signaling pathway markedly suppressed the enhanced migration and adhesion of HUVECs induced by CORM-2.
Conclusion CORM-2 could promote endothelial repair, and inhibit neointima formation after carotid artery balloon injury, which might be associated with the function changes of HUVECs regulated by PI3K/Akt/eNOS pathway.

English Abstract

HU Qing Song, CHEN Yang Xin, HUANG Qing Sheng, DENG Bing Qing, XIE Shuang Lun, WANG Jing Feng, NIE Ru Qiong. Carbon Monoxide Releasing Molecule Accelerates Reendothelialization after Carotid Artery Balloon Injury in Rat[J]. Biomedical and Environmental Sciences, 2015, 28(4): 253-262. doi: 10.3967/bes2015.036
Citation: HU Qing Song, CHEN Yang Xin, HUANG Qing Sheng, DENG Bing Qing, XIE Shuang Lun, WANG Jing Feng, NIE Ru Qiong. Carbon Monoxide Releasing Molecule Accelerates Reendothelialization after Carotid Artery Balloon Injury in Rat[J]. Biomedical and Environmental Sciences, 2015, 28(4): 253-262. doi: 10.3967/bes2015.036

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