Volume 31 Issue 2
Feb.  2018
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Hajer Ben Saad, Dorra Driss, Imen Jaballi, Hanen Ghozzi, Ons Boudawara, Michael Droguet, Christian Magné, Monsef Nasri, Khaled Mounir Zeghal, Ahmed Hakim, Ibtissem Ben Amara. Potassium Bromate-induced Changes in the Adult Mouse Cerebellum Are Ameliorated by Vanillin[J]. Biomedical and Environmental Sciences, 2018, 31(2): 115-125. doi: 10.3967/bes2018.014
Citation: Hajer Ben Saad, Dorra Driss, Imen Jaballi, Hanen Ghozzi, Ons Boudawara, Michael Droguet, Christian Magné, Monsef Nasri, Khaled Mounir Zeghal, Ahmed Hakim, Ibtissem Ben Amara. Potassium Bromate-induced Changes in the Adult Mouse Cerebellum Are Ameliorated by Vanillin[J]. Biomedical and Environmental Sciences, 2018, 31(2): 115-125. doi: 10.3967/bes2018.014

Potassium Bromate-induced Changes in the Adult Mouse Cerebellum Are Ameliorated by Vanillin

doi: 10.3967/bes2018.014

The present work was supported by the DGRST grant (Direction Générale de la Recherche Scientifique et Technique, Tunisie) UR/12 ES-13

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  • Corresponding author: Hajer Ben Saad, Tel:216-74-241888, Fax:216-74-246217, E-mail:hajer.ben.saad@hotmail.fr
  • Received Date: 2017-07-07
  • Accepted Date: 2017-11-06
  • Objective The current study aimed to elucidate the effect of vanillin on behavioral changes, oxidative stress, and histopathological changes induced by potassium bromate (KBrO3), an environmental pollutant, in the cerebellum of adult mice. Methods The animals were divided into four groups:group 1 served as a control, group 2 received KBrO3, group 3 received KBrO3 and vanillin, and group 4 received only vanillin. We then measured behavioral changes, oxidative stress, and molecular and histological changes in the cerebellum. Results We observed significant behavioral changes in KBrO3-exposed mice. When investigating redox homeostasis in the cerebellum, we found that mice treated with KBrO3 had increased lipid peroxidation and protein oxidation in the cerebellum. These effects were accompanied by decreased Na+-K+ and Mg2+ ATPase activity and antioxidant enzyme gene expression when compared to the control group. Additionally, there was a significant increase in cytokine gene expression in KBrO3-treated mice. Microscopy revealed that KBrO3 intoxication resulted in numerous degenerative changes in the cerebellum that were substantially ameliorated by vanillin supplementation. Co-administration of vanillin blocked the biochemical and molecular anomalies induced by KBrO3. Conclusion Our results demonstrate that vanillin is a potential therapeutic agent for oxidative stress associated with neurodegenerative diseases.
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