Volume 31 Issue 2
Feb.  2018
Turn off MathJax
Article Contents

Hajer Ben Saad, Dorra Driss, Imen Jaballi, Hanen Ghozzi, Ons Boudawara, Michael Droguet, Christian Magné, Monsef Nasri, Khaled Mounir Zeghal, Ahmed Hakim, Ibtissem Ben Amara. Potassium Bromate-induced Changes in the Adult Mouse Cerebellum Are Ameliorated by Vanillin[J]. Biomedical and Environmental Sciences, 2018, 31(2): 115-125. doi: 10.3967/bes2018.014
Citation: Hajer Ben Saad, Dorra Driss, Imen Jaballi, Hanen Ghozzi, Ons Boudawara, Michael Droguet, Christian Magné, Monsef Nasri, Khaled Mounir Zeghal, Ahmed Hakim, Ibtissem Ben Amara. Potassium Bromate-induced Changes in the Adult Mouse Cerebellum Are Ameliorated by Vanillin[J]. Biomedical and Environmental Sciences, 2018, 31(2): 115-125. doi: 10.3967/bes2018.014

Potassium Bromate-induced Changes in the Adult Mouse Cerebellum Are Ameliorated by Vanillin

doi: 10.3967/bes2018.014
Funds:

The present work was supported by the DGRST grant (Direction Générale de la Recherche Scientifique et Technique, Tunisie) UR/12 ES-13

More Information
  • Corresponding author: Hajer Ben Saad, Tel:216-74-241888, Fax:216-74-246217, E-mail:hajer.ben.saad@hotmail.fr
  • Received Date: 2017-07-07
  • Accepted Date: 2017-11-06
  • Objective The current study aimed to elucidate the effect of vanillin on behavioral changes, oxidative stress, and histopathological changes induced by potassium bromate (KBrO3), an environmental pollutant, in the cerebellum of adult mice. Methods The animals were divided into four groups:group 1 served as a control, group 2 received KBrO3, group 3 received KBrO3 and vanillin, and group 4 received only vanillin. We then measured behavioral changes, oxidative stress, and molecular and histological changes in the cerebellum. Results We observed significant behavioral changes in KBrO3-exposed mice. When investigating redox homeostasis in the cerebellum, we found that mice treated with KBrO3 had increased lipid peroxidation and protein oxidation in the cerebellum. These effects were accompanied by decreased Na+-K+ and Mg2+ ATPase activity and antioxidant enzyme gene expression when compared to the control group. Additionally, there was a significant increase in cytokine gene expression in KBrO3-treated mice. Microscopy revealed that KBrO3 intoxication resulted in numerous degenerative changes in the cerebellum that were substantially ameliorated by vanillin supplementation. Co-administration of vanillin blocked the biochemical and molecular anomalies induced by KBrO3. Conclusion Our results demonstrate that vanillin is a potential therapeutic agent for oxidative stress associated with neurodegenerative diseases.
  • 加载中
  • [1] Holmes G. Clinical symptoms of cerebellar disease and their interpretation (Croonian lectureⅢ). The Lancet, 1922; 59565. https://www.researchgate.net/publication/227085607_Clinical_symptoms_of_cerebellar_disease_and_their_interpretation
    [2] Mariën P. A Role for the Cerebellum in Language and Related Cognitive and Affective Functions. In: Mody M. (eds) Neural Mechanisms of Language. Innovations in Cognitive Neuroscience. Springer, Boston, MA. 2017.
    [3] Atif F, Yousuf S, Agrawal SK. Restraint stress-induced oxidative damage and its amelioration with selenium. Eur J Pharmacol, 2008; 600, 59-63. doi:  10.1016/j.ejphar.2008.09.029
    [4] Ben Amara I, Fetoui H, Guermazi F, et al. Dietary selenium addition improves cerebrum and cerebellum impairments induced by methimazole in suckling rats. Inter J Dev Neurosci, 2009; 27, 719-26. doi:  10.1016/j.ijdevneu.2009.07.002
    [5] Deng H, Henatati A, Tainer J, et al. Amyotrophic lateral sclerosis and structural defects in Cu, Zn superoxide dismutase. Science, 1993; 261, 1047-51. doi:  10.1126/science.8351519
    [6] Smith MA, Rudinicka-Nawrot M, Richey PL, et al. Carbonyl-related posttranslational modification of neurofilament protein in the neurofibrillary pathology of Alzheimer's disease. J Neurochem, 1995; 64, 2660-6. doi:  10.1046/j.1471-4159.1995.64062660.x/abstract
    [7] Becaria A, Bondy SC, Campbell A. Aluminum and copper interact in the promotion of oxidative but not inflammatory events:implications for Alzheimer's disease. J Alzheimer Dis, 2003; 5, 31-8. doi:  10.3233/JAD-2003-5105
    [8] Frackowick J, Sukontasup T, Potempska. Lysosomal deposition of Abeta in cultures of brain vascular smooth muscle cells is enhanced by iron. Brain Res, 2004; 1002, 67-75. doi:  10.1016/j.brainres.2003.12.015
    [9] Needham LL, Grandjean P, Heinzow B, et al. Partition of environmental chemicals between maternal and fetal blood and tissues. Environ Sci Technol, 2011; 45, 1121-6. doi:  10.1021/es1019614
    [10] Matsumoto I, Morizono T, Paparella MM. Hearing loss following potassium bromate:two case reports. Otolaryngol Head Neck Surg, 1980; 88, 625-9. doi:  10.1177/019459988008800519
    [11] Cho DH, Hong JT, Chin K, et al. Organotropic formation and disappearance of 8-hydroxydeoxyguanosine in the kidney of Sprague-Dawley rats exposed to adriamycin and KBrO3. Cancer Lett, 1993; 74, 141-5. doi:  10.1016/0304-3835(93)90235-2
    [12] Muratsuka Y, Ueda H, Konishi T. Effects of sodium bromate on ionic concentrations and osmolalities of the cochlear fluids in guinea pigs. Hearing Res, 1989; 39, 241-50. doi:  10.1016/0378-5955(89)90044-0
    [13] Bythrow JD. Vanillin as a medical plant. Sem Int Med, 2005; 3, 129-31. doi:  10.1016/j.sigm.2006.03.001
    [14] Jiankang L, Akitane M. Antioxidant and pro-oxidant activities of p-hydroxybenzyl alcohol and vanillin:Effects on free radicals, brain peroxidation and degradation of benzoate, deoxyribose, amino acids and DNA. Neuropharmacology, 1993; 32, 659-69. doi:  10.1016/0028-3908(93)90079-I
    [15] Takeda S, Aburada M. The choleretic mechanism of coumarin compounds and phenolic compounds. J Pharmacobiodyn, 1981; 4, 724-34. doi:  10.1248/bpb1978.4.724
    [16] King AA, Shaughnessy T, Mure K, et al. Antimutagenicity of cinnamaldehyde and vanillin in human cells:Global gene expression and possible role of DNA damage and repair. Mut Res, 2007; 616, 60-9. doi:  10.1016/j.mrfmmm.2006.11.022
    [17] Pereira Bezerra D, Nascimento Soares AK, Damiao Pergentino D. Overview of the Role of Vanillin on Redox Status and Cancer Development. Oxi Med Cell Long, 2016; 9734, 816-9. https://www.ncbi.nlm.nih.gov/pubmed/28077989
    [18] Ben Saad H, Driss D, Ben Amara I, et al. Altered hepatic mRNA expression of immune response-associated DNA damage in mice liver induced by potassium bromate:Protective role of vanillin. Environ Toxicol, 2015; 21, 10-22181. doi:  10.1002/tox.22181/abstract
    [19] Ben Saad H, Gargouri M, Kallel F, et al. Flavonoid compounds from the red marine alga Alsidium corallinum protect against potassium bromate-induced nephrotoxicity in adult mice. Environ Toxicol, 2016; 10, 22368. doi:  10.1002/tox.22368/full
    [20] Dhanalakshmi Ch, Manivasagam Th, Nataraj J, et al. Neurosupportive Role of Vanillin, a Natural Phenolic Compound, on Rotenone Induced Neurotoxicity in SH-SY5Y. Neuroblastoma. Cells Evid Bas Complem Alter Med, 2015; 626028, 11.
    [21] Council of European Communities. Council instructions about the protection of living animals used in scientific investigations. Off J Eur Com, 1986; (JO 86/609/CEE), L358, 1-18. http://ec.europa.eu/environment/chemicals/lab_animals/legislation_en.htm
    [22] Arai T, Kelly VP, Minowa O, et al. The study using wild-type and Ogg1 knockout mice exposed to potassium bromate shows no tumor induction despite an extensive accumulation of 8-hydroxyguanine in kidney DNA. Toxicology, 2006; 221, 179-86. doi:  10.1016/j.tox.2006.01.004
    [23] Maury DK, Adhikari S, Nair CK, et al. DNA protective properties of vanillin against γ-radiation under different conditions:Possible mechanisms. Mut Res, 2007; 634, 69-80. doi:  10.1016/j.mrgentox.2007.06.003
    [24] Kulkarni SK. Handbook of Experimental Pharmacology. 3rd Edn, Vallabh Prakashan, New Delhi, 1999.
    [25] Fan LW, Chen RF, Mitchell HJ, et al. alpha-Phenyl-n-tert-butyl-nitrone attenuates lipopolysaccharide-induced brain injury and improves neurological reflexes and early sensorimotor behavioral performance in juvenile rats. J Neurosc Res, 2008; 86, 3536-47. doi:  10.1002/jnr.v86:16
    [26] Brown RE, Corey SC, Moore AK. Differences in measures of exploration and fear in MHC-congenic C57BL/6J and B6-H-2Kmice. Beh Gen, 1999; 26, 263-71. doi:  10.1023/A:1021694307672
    [27] Sumathi T, Asha D, Nagarajan G, et al. L-theanine alleviates the neuropathological changes induced by PCB (Aroclor 1254) via inhibiting upregulation of inflammatory cytokines and oxidative stress in rat brain. Envir Toxicol Pharmacol, 2016; 42, 99-117. doi:  10.1016/j.etap.2016.01.008
    [28] Lowry OH, Rosenbrough NJ, Farr AL. Protein measurement with the Folin phenol reagent. J Biol Chem, 1951; 193, 265-75. https://www.ncbi.nlm.nih.gov/pubmed/14907713
    [29] Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods. Enzymol, 1990; 186, 421-31. doi:  10.1016/0076-6879(90)86135-I
    [30] Ou P, Wolff SP. A discontinuous method for catalase determination at near physiological concentrations of H2O2 and its application to the study of H2O2 fluxes within cells. J Biochem Biophys Methods, 1996; 31, 59-67. doi:  10.1016/0165-022X(95)00039-T
    [31] Jiang ZY, Hunt JV, Wolff SP. Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxides in low density lipoprotein. Anal Biochem, 1992; 202, 384-9. doi:  10.1016/0003-2697(92)90122-N
    [32] Witko V, Nguyen AT, Descamps-Latscha B. Microtiter plate assay for phagocytederived taurine chloramines. J Clin Lab Anal, 1992; 6, 47-53. doi:  10.1002/(ISSN)1098-2825
    [33] Beauchamp C, Fridovich I. Superoxydedimutase:improved assays and an assay applicable to acrylamide gel. Anal Biochem, 1971; 44, 276-87. doi:  10.1016/0003-2697(71)90370-8
    [34] Flohe L, Gunzler WA. Assays of glutathione peroxidase. Methods Enzymol, 1984; 105, 114-21. doi:  10.1016/S0076-6879(84)05015-1
    [35] Kawamoto EM, Munhoz CD, Glezer I, et al. Oxidative state in platelets and erythrocytes in aging and Alzheimer's disease. Neurobiol Aging, 2005; 26, 857-64. doi:  10.1016/j.neurobiolaging.2004.08.011
    [36] Gabe M. Techniques histologiques. Masson, Paris, 1968; 838-79. http://www.researchgate.net/publication/50339229_Techniques_histologiques
    [37] Farina M, Aschner M, Rocha JB. Oxidative stress in MeHg induced neurotoxicity. Toxicol Appl Pharma, 2011a; 256, 405-17. doi:  10.1016/j.taap.2011.05.001
    [38] Farina M, Rocha JB, Aschner M. Mechanisms of methylmercury-induced neurotoxicity:evidence from experimental studies. Life Sci, 2011b; 89, 555-63. doi:  10.1016/j.lfs.2011.05.019
    [39] Cheng WY, Hsiang CY, Bau DT. Microarray analysis of vanillin regulated gene expression profile in human hepatocarcinoma. Cells Pharm Res, 2007; 56, 474-82. doi:  10.1016/j.phrs.2007.09.009
    [40] Adedara IA, Rosemberg DB, Souza DO, et al. Biochemical and behavioral deficits in lobster cockroach Nauphoetacinerea model of methylmercury exposure. Toxicol Res, 2015; 4, 442-51. doi:  10.1039/C4TX00231H
    [41] Gupta VB, Anitha S, Hegdea ML, et al. Aluminium in Alzheimer's disease:are we still at a crossroad? Cell Mol Life Sci, 2005; 62, 143-58. doi:  10.1007/s00018-004-4317-3
    [42] El-Sayed el-SM, Abo-Salem OM, Abd-Ellah MF, et al. Hesperidin, an antioxidant flavonoid, prevents acrylonitrile-induced oxidative stress in rat brain. J Biochem Mol Toxicol, 2008; 22, 268-73. doi:  10.1002/jbt.v22:4
    [43] Hawkins CL, Davies MJ. Generation and propagation of radical reactions on proteins. Biochim Biophys Acta, 2001; 1504, 196. doi:  10.1016/S0005-2728(00)00252-8
    [44] Halliwell B, Chirico S. Lipid peroxidation:its mechanism, measurement, and significance. Am J Clin Nutr, 1993; 57, 715-24. doi:  10.1093/ajcn/57.5.715S
    [45] Anane R, Creppy EE. Lipid peroxidation as pathway of aluminium cytotoxicity in human skin fibroblast cultures:prevention by superoxide dismutase +-catalase and vitamins E and C. Hum Exp Toxicol, 2001; 20, 477-81. doi:  10.1191/096032701682693053
    [46] Ben Saad H, Driss D, Ellouz-Chaabouni S, et al. Vanillin mitigates potassium bromate-induced molecular, biochemical and histopathological changes in the kidney of adult mice. Chem Biol Inter, 2016; 252, 102-13. doi:  10.1016/j.cbi.2016.04.015
    [47] Samson FE, Nelson SR. The aging brain, metals and oxygen free radicals. Cell Mol Biol, 2000; 46, 699-707. http://www.ncbi.nlm.nih.gov/pubmed/10875433
    [48] Chen KN, Peng WH, Hou CW, et al. Codonopsis javanica root extracts attenuate hyperinsulinemia and lipid peroxidation in fructose-fed insulin resistant rats. J Food Drug Anal, 2013; 21, 347-55. doi:  10.1016/j.jfda.2013.08.001
    [49] Farina M, Franco JL, Ribas CM, et al. Protective effects of Polygala paniculata extract against methylmercury-induced neurotoxicity in mice. J Pharm Pharmacol, 2005; 57, 1503-8. doi:  10.1211/jpp.57.11.0017
    [50] Rahimi R, Abdollahi M. A review on the mechanisms involved inhyperglycemia induced by organophosphorus pesticides. Pestic Biochem Physiol, 2007; 88, 115-21. doi:  10.1016/j.pestbp.2006.10.003
    [51] Lingrel JB. Na+, K+-ATPase:isoform structure, function, and expression. J Bioenerg Biomembr, 1992; 24, 263-70. http://www.ncbi