2015 Vol. 28, No. 1

Select articles
In vitro and In vivo Evaluation of the Developed PLGA/HAp/Zein Scaffolds for Bone-Cartilage Interface Regeneration
LIN Yong Xin, DING Zhi Yong, ZHOU Xiao Bin, LI Si Tao, XIE De Ming, LI Zhi Zhong, SUN Guo Dong
2015, 28(1): 1-12. doi: 10.3967/bes2015.001
Objective To investigate the effect of electronspun PLGA/HAp/Zein scaffolds on the repair of cartilage defects.
Methods The PLGA/HAp/Zein composite scaffolds were fabricated by electrospinning method. The physiochemical properties and biocompatibility of the scaffolds were separately characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), and fourier transform infrared spectroscopy (FTIR), human umbilical cord mesenchymal stem cells (hUC-MSCs) culture and animal experiments.
Results The prepared PLGA/HAp/Zein scaffolds showed fibrous structure with homogenous distribution. hUC-MSCs could attach to and grow well on PLGA/HAp/Zein scaffolds, and there was no significant difference between cell proliferation on scaffolds and that without scaffolds (P>0.05). The PLGA/HAp/Zein scaffolds possessed excellent ability to promote in vivo cartilage formation. Moreover, there was a large amount of immature chondrocytes and matrix with cartilage lacuna on PLGA/HAp/Zein scaffolds.
Conclusion The data suggest that the PLGA/HAp/Zein scaffolds possess good biocompatibility, which are anticipated to be potentially applied in cartilage tissue engineering and reconstruction.
Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway
XIONG Lu, SUN Cheng Feng, ZHANG Jing, GAO Ya Bing, WANG Li Feng, ZUO Hong Yan, WANG Shui Ming, ZHOU Hong Mei, XU Xin Ping, DONG Ji, YAO Bin Wei, ZHAO Li, PENG Rui Yun
2015, 28(1): 13-24. doi: 10.3967/bes2015.002
Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor (NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment.
Methods 48 male Wistar rats were exposed to 30 mW/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 mW/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated.
Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits (NR1, NR2A, and NR2B) and related signaling molecules (Ca2+/calmodulin-dependent kinase II gamma and phosphorylated cAMP-response element binding protein) were examined.
Conclusion 30 mW/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity.
Rapid Detection of Rifampin-resistant Clinical Isolates of Mycobacterium tuberculosis by Reverse Dot Blot Hybridization
GUO Qian, YU Yan, ZHU Yan Ling, ZHAO Xiu Qin, LIU Zhi Guang, ZHANG Yuan Yuan, LI Gui Lian, WEI Jian Hao, WU Yi Mou, WAN Kang Lin
2015, 28(1): 25-35. doi: 10.3967/bes2015.003
Objective A PCR-reverse dot blot hybridization (RDBH) assay was developed for rapid detection of rpoB gene mutations in‘hot mutation region’ of Mycobacterium tuberculosis (M. tuberculosis).
Methods 12 oligonucleotide probes based on the wild-type and mutant genotype rpoB sequences of M. tuberculosis were designed to screen the most frequent wild-type and mutant genotypes for diagnosing RIF resistance. 300 M. tuberculosis clinical isolates were detected by RDBH, conventional drug-susceptibility testing (DST) and DNA sequencing to evaluate the RDBH assay.
Results The sensitivity and specificity of the RDBH assay were 91.2%(165/181) and 98.3%(117/119), respectively, as compared to DST. When compared with DNA sequencing, the accuracy, positive predictive value (PPV) and negative predictive value (NPV) of the RDBH assay were 97.7%(293/300), 98.2%(164/167), and 97.0%(129/133), respectively. Furthermore, the results indicated that the most common mutations were in codons 531 (48.6%), 526 (25.4%), 516 (8.8%), and 511 (6.6%), and the combinative mutation rate was 15 (8.3%). One and two strains of insertion and deletion were found among all strains, respectively.
Conclusion Our findings demonstrate that the RDBH assay is a rapid, simple and sensitive method for diagnosing RIF-resistant tuberculosis.
Cytoprotective Effect of Silymarin against Diabetes-Induced Cardiomyocyte Apoptosis in Diabetic Rats
Muobarak J Tuorkey, Nabila I El-Desouki, Rabab A Kamel
2015, 28(1): 36-43. doi: 10.3967/bes2015.004
Objective The beneficial effects of silymarin have been extensively studied in the context of inflammation and cancer treatment, yet much less is known about its therapeutic effect on diabetes. The present study was aimed to investigate the cytoprotective activity of silymarin against diabetes-induced cardiomyocyte apoptosis.
Methods Rats were randomly divided into: control group, untreated diabetes group and diabetes group treated with silymarin (120 mg/kg·d) for 10 d. Rats were sacrificed, and the cardiac muscle specimens and blood samples were collected. The immunoreactivity of caspase-3 and Bcl-2 in the cardiomyocytes was measured. Total proteins, glucose, insulin, creatinine, AST, ALT, cholesterol, and triglycerides levels were estimated.
Results Unlike the treated diabetes group, cardiomyocyte apoptosis increased in the untreated rats, as evidenced by enhanced caspase-3 and declined Bcl-2 activities. The levels of glucose, creatinine, AST, ALT, cholesterol, and triglycerides declined in the treated rats. The declined levels of insulin were enhanced again after treatment of diabetic rats with silymarin, reflecting a restoration of the pancreaticβ-cells activity.
Conclusion The findings of this study are of great importance, which confirmed for the first time that treatment of diabetic subjects with silymarin may protect cardiomyocytes against apoptosis and promote survival-restoration of the pancreaticβ-cells.
The Pollution Character Analysis and Risk Assessment for Metals in Dust and PM10 around Road from China
CHEN Rong Hui, WANG Bao Qing#, WANG Ze Bei, , YAO Shu
2015, 28(1): 44-56. doi: 10.3967/bes2015.005
Bone Injury and Fracture Healing Biology
Ahmad Oryan, Somayeh Monazzah, Amin Bigham-Sadegh
2015, 28(1): 57-71. doi: 10.3967/bes2015.006
Pathological Changes in the Sinoatrial Node Tissues of Rats Caused by Pulsed Microwave Exposure
LIU Yan Qing, GAO Ya Bing, DONG Ji, YAO Bin Wei, ZHAO Li, PENG Rui Yun
2015, 28(1): 72-75. doi: 10.3967/bes2015.007
Ambient Temperature and Outpatient Visits for Acute Exacerbation of Chronic Bronchitis in Shanghai:A Time Series Analysis
HUANG Fang, ZHAO Ang, CHEN Ren Jie, KAN Hai Dong, KUANG Xing Ya
2015, 28(1): 76-79. doi: 10.3967/bes2015.008
Assessing the Effectiveness of a Cervical Cancer Screening Program in a Hospital-based Study
YANG Yi, LANG Jing He, WANG You Fang, CHENG Xue Mei, CAI Yu Pin, LI Hui, ZHU Bao Li, ZHANG Rui Fen
2015, 28(1): 80-84. doi: 10.3967/bes2015.009
In vivo Digestive Stability of Soybean?-conglycinin?-subunit in WZS Minipigs
HUANG Qiong, XU Hai Bin, YU Zhou, LIU Shan, GAO Peng
2015, 28(1): 85-88. doi: 10.3967/bes2015.010