QI YuHong; LIANG Jun; HUI YanPing; DING GuiRong; LIU JunYe; SU XiaoMing; GUO GuoZhen

doi:10.3967/0895-3988.2011.01.016

Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses

QI YuHong, LIANG Jun, HUI YanPing, DING GuiRong, LIU JunYe, SU XiaoMing, GUO GuoZhen

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(5): 560-568

QI YuHong, LIANG Jun, HUI YanPing, DING GuiRong, LIU JunYe, SU XiaoMing, GUO GuoZhen. Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses[J]. Biomedical and Environmental Sciences, 2011, 24(5): 560-568. doi: 10.3967/0895-3988.2011.01.016

Citation:

QI YuHong, LIANG Jun, HUI YanPing, DING GuiRong, LIU JunYe, SU XiaoMing, GUO GuoZhen. Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses[J]. Biomedical and Environmental Sciences, 2011, 24(5): 560-568. doi: 10.3967/0895-3988.2011.01.016

doi: 10.3967/0895-3988.2011.01.016

基金项目: This study was supported by the Research Fund of the National Natural Science Foundation of China(No:60871068%30970670)

Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses

Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi,China;Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University,Xi'an 710032, Shaanxi, China
Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi,China
Department of Pathology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi 'an 710032, Shaanxi, China

Funds: This study was supported by the Research Fund of the National Natural Science Foundation of China(No:60871068%30970670)

摘要: Objective We examined alterations in the expression of tumorigenesis-related genes in the pituitary gland of rats exposed to electromagnetic pulses (EMP).Methods The global gene expression profiles of the pituitary gland in EMP-exposed and control groups were detected by cDNA microarray analysis.We then validated and further investigated the reduced expression of two tumorigenesis-related genes,Pten,and Jund,by assessing their mRNA and protein expression by quantitative real-time-PCR,western blotting,and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.Results EMP exposure induced genome-wide gene expression changes in the rat pituitary gland.There was decreased expression of the Pten and Jund mRNAs and proteins in EMP-exposed rats compared with in unexposed control animals.Conclusion EMP exposure alters the expression of tumorigenesis-related genes in the pituitary gland.These tumorigenesis-related genes are potentially involved in the development of pituitary gland tumors in rats.
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Abstract: Objective We examined alterations in the expression of tumorigenesis-related genes in the pituitary gland of rats exposed to electromagnetic pulses (EMP).Methods The global gene expression profiles of the pituitary gland in EMP-exposed and control groups were detected by cDNA microarray analysis.We then validated and further investigated the reduced expression of two tumorigenesis-related genes,Pten,and Jund,by assessing their mRNA and protein expression by quantitative real-time-PCR,western blotting,and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.Results EMP exposure induced genome-wide gene expression changes in the rat pituitary gland.There was decreased expression of the Pten and Jund mRNAs and proteins in EMP-exposed rats compared with in unexposed control animals.Conclusion EMP exposure alters the expression of tumorigenesis-related genes in the pituitary gland.These tumorigenesis-related genes are potentially involved in the development of pituitary gland tumors in rats.
- Tumorigenesis /
- Electromagnetic pulses /
- Pituitary gland /
- Pten /
- Jund

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Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses

doi: 10.3967/0895-3988.2011.01.016

基金项目: This study was supported by the Research Fund of the National Natural Science Foundation of China(No:60871068%30970670)

刊出日期: 2011-10-20

关键词:

Tumorigenesis /

Electromagnetic pulses /

Pituitary gland /

Pten /

Jund

摘要: Objective We examined alterations in the expression of tumorigenesis-related genes in the pituitary gland of rats exposed to electromagnetic pulses (EMP).Methods The global gene expression profiles of the pituitary gland in EMP-exposed and control groups were detected by cDNA microarray analysis.We then validated and further investigated the reduced expression of two tumorigenesis-related genes,Pten,and Jund,by assessing their mRNA and protein expression by quantitative real-time-PCR,western blotting,and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.Results EMP exposure induced genome-wide gene expression changes in the rat pituitary gland.There was decreased expression of the Pten and Jund mRNAs and proteins in EMP-exposed rats compared with in unexposed control animals.Conclusion EMP exposure alters the expression of tumorigenesis-related genes in the pituitary gland.These tumorigenesis-related genes are potentially involved in the development of pituitary gland tumors in rats.

English Abstract

Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses

Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi,China;Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University,Xi'an 710032, Shaanxi, China

Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi,China

Department of Pathology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi 'an 710032, Shaanxi, China

Funds: This study was supported by the Research Fund of the National Natural Science Foundation of China(No:60871068%30970670)

Publish Date: 2011-10-20

Keywords:

Abstract: Objective We examined alterations in the expression of tumorigenesis-related genes in the pituitary gland of rats exposed to electromagnetic pulses (EMP).Methods The global gene expression profiles of the pituitary gland in EMP-exposed and control groups were detected by cDNA microarray analysis.We then validated and further investigated the reduced expression of two tumorigenesis-related genes,Pten,and Jund,by assessing their mRNA and protein expression by quantitative real-time-PCR,western blotting,and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.Results EMP exposure induced genome-wide gene expression changes in the rat pituitary gland.There was decreased expression of the Pten and Jund mRNAs and proteins in EMP-exposed rats compared with in unexposed control animals.Conclusion EMP exposure alters the expression of tumorigenesis-related genes in the pituitary gland.These tumorigenesis-related genes are potentially involved in the development of pituitary gland tumors in rats.

Citation:

QI YuHong, LIANG Jun, HUI YanPing, DING GuiRong, LIU JunYe, SU XiaoMing, GUO GuoZhen. Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses[J]. Biomedical and Environmental Sciences, 2011, 24(5): 560-568. doi: 10.3967/0895-3988.2011.01.016