QIU LianBo; CHEN Chen; DING GuiRong; ZHOU Yan; ZHANG MengYao

doi:10.3967/0895-3988.2011.04.016

The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats

QIU LianBo, CHEN Chen, DING GuiRong, ZHOU Yan, ZHANG MengYao

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(4): 438-444

QIU LianBo, CHEN Chen, DING GuiRong, ZHOU Yan, ZHANG MengYao. The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats[J]. Biomedical and Environmental Sciences, 2011, 24(4): 438-444. doi: 10.3967/0895-3988.2011.04.016

Citation:

QIU LianBo, CHEN Chen, DING GuiRong, ZHOU Yan, ZHANG MengYao. The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats[J]. Biomedical and Environmental Sciences, 2011, 24(4): 438-444. doi: 10.3967/0895-3988.2011.04.016

doi: 10.3967/0895-3988.2011.04.016

基金项目: the Research Fund of Scientific and technological research and Development Program of Shannxi province of China(2010K16-04-04)

The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats

Department of Blood Transfusion, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shannxi,China
Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University,Xi'an 710032, Shannxi, China
Department of Chemistry, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, Shannxi, China

Funds: the Research Fund of Scientific and technological research and Development Program of Shannxi province of China(2010K16-04-04)

摘要: Objective To investigate changes in the expression of tight junction (TJ) proteins in the cerebral cortex,hippocampus,heart,lung,and testes of rats after exposure to electromagnetic pulse (EMP).Methods Eighteen adult male Sprague-Dawley rats were divided into sham and exposure groups.The exposure groups received EMP at 200 kV/m for 200 pulses with a repetition rate of 1 Hz.The expression of TJ proteins (ZO-1,occludin,actin) in the several organs was examined by western blotting.Results ZO-1 levels in the cerebral cortex decreased 1 h and 3 h after EMP exposure compared with sham group (P＜0.05).No significant difference was observed for occludin and actin.ZO-1 levels in the hippocampus increased 1 h and 3 h post-exposure (P＜0.05),and occludin decreased after 3 h (P＜0.05);however,actin was unaffected.ZO-1 levels in the heart increased 3 h post-exposure (P＜0.05),occludin decreased 3 h post-exposure (P＜0.05),and actin increased 1 h and 3 h post-exposure (P＜0.05).ZO-1,occludin and actin levels in the lung decreased compared with those in the sham group (P＜0.05).ZO-1 and occludin levels in the testes decreased 1 h and 3 h post-exposure (P＜0.05),but actin showed no significant change.Conclusion Exposure to EMP altered the expression levels of TJ proteins,particularly ZO-1,in the organs of adult male rats,which may induce changes in barrier structure and function.
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Abstract: Objective To investigate changes in the expression of tight junction (TJ) proteins in the cerebral cortex,hippocampus,heart,lung,and testes of rats after exposure to electromagnetic pulse (EMP).Methods Eighteen adult male Sprague-Dawley rats were divided into sham and exposure groups.The exposure groups received EMP at 200 kV/m for 200 pulses with a repetition rate of 1 Hz.The expression of TJ proteins (ZO-1,occludin,actin) in the several organs was examined by western blotting.Results ZO-1 levels in the cerebral cortex decreased 1 h and 3 h after EMP exposure compared with sham group (P＜0.05).No significant difference was observed for occludin and actin.ZO-1 levels in the hippocampus increased 1 h and 3 h post-exposure (P＜0.05),and occludin decreased after 3 h (P＜0.05);however,actin was unaffected.ZO-1 levels in the heart increased 3 h post-exposure (P＜0.05),occludin decreased 3 h post-exposure (P＜0.05),and actin increased 1 h and 3 h post-exposure (P＜0.05).ZO-1,occludin and actin levels in the lung decreased compared with those in the sham group (P＜0.05).ZO-1 and occludin levels in the testes decreased 1 h and 3 h post-exposure (P＜0.05),but actin showed no significant change.Conclusion Exposure to EMP altered the expression levels of TJ proteins,particularly ZO-1,in the organs of adult male rats,which may induce changes in barrier structure and function.
- Electromagnetic pulse /
- Tight junction /
- ZO-1 /
- Occludin /
- Actin

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The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats

doi: 10.3967/0895-3988.2011.04.016

基金项目: the Research Fund of Scientific and technological research and Development Program of Shannxi province of China(2010K16-04-04)

刊出日期: 2011-08-20

关键词:

Electromagnetic pulse /

Tight junction /

ZO-1 /

Occludin /

Actin

摘要: Objective To investigate changes in the expression of tight junction (TJ) proteins in the cerebral cortex,hippocampus,heart,lung,and testes of rats after exposure to electromagnetic pulse (EMP).Methods Eighteen adult male Sprague-Dawley rats were divided into sham and exposure groups.The exposure groups received EMP at 200 kV/m for 200 pulses with a repetition rate of 1 Hz.The expression of TJ proteins (ZO-1,occludin,actin) in the several organs was examined by western blotting.Results ZO-1 levels in the cerebral cortex decreased 1 h and 3 h after EMP exposure compared with sham group (P＜0.05).No significant difference was observed for occludin and actin.ZO-1 levels in the hippocampus increased 1 h and 3 h post-exposure (P＜0.05),and occludin decreased after 3 h (P＜0.05);however,actin was unaffected.ZO-1 levels in the heart increased 3 h post-exposure (P＜0.05),occludin decreased 3 h post-exposure (P＜0.05),and actin increased 1 h and 3 h post-exposure (P＜0.05).ZO-1,occludin and actin levels in the lung decreased compared with those in the sham group (P＜0.05).ZO-1 and occludin levels in the testes decreased 1 h and 3 h post-exposure (P＜0.05),but actin showed no significant change.Conclusion Exposure to EMP altered the expression levels of TJ proteins,particularly ZO-1,in the organs of adult male rats,which may induce changes in barrier structure and function.

English Abstract

The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats

Department of Blood Transfusion, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shannxi,China

Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University,Xi'an 710032, Shannxi, China

Department of Chemistry, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, Shannxi, China

Funds: the Research Fund of Scientific and technological research and Development Program of Shannxi province of China(2010K16-04-04)

Publish Date: 2011-08-20

Keywords:

Abstract: Objective To investigate changes in the expression of tight junction (TJ) proteins in the cerebral cortex,hippocampus,heart,lung,and testes of rats after exposure to electromagnetic pulse (EMP).Methods Eighteen adult male Sprague-Dawley rats were divided into sham and exposure groups.The exposure groups received EMP at 200 kV/m for 200 pulses with a repetition rate of 1 Hz.The expression of TJ proteins (ZO-1,occludin,actin) in the several organs was examined by western blotting.Results ZO-1 levels in the cerebral cortex decreased 1 h and 3 h after EMP exposure compared with sham group (P＜0.05).No significant difference was observed for occludin and actin.ZO-1 levels in the hippocampus increased 1 h and 3 h post-exposure (P＜0.05),and occludin decreased after 3 h (P＜0.05);however,actin was unaffected.ZO-1 levels in the heart increased 3 h post-exposure (P＜0.05),occludin decreased 3 h post-exposure (P＜0.05),and actin increased 1 h and 3 h post-exposure (P＜0.05).ZO-1,occludin and actin levels in the lung decreased compared with those in the sham group (P＜0.05).ZO-1 and occludin levels in the testes decreased 1 h and 3 h post-exposure (P＜0.05),but actin showed no significant change.Conclusion Exposure to EMP altered the expression levels of TJ proteins,particularly ZO-1,in the organs of adult male rats,which may induce changes in barrier structure and function.

Citation:

QIU LianBo, CHEN Chen, DING GuiRong, ZHOU Yan, ZHANG MengYao. The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats[J]. Biomedical and Environmental Sciences, 2011, 24(4): 438-444. doi: 10.3967/0895-3988.2011.04.016