JIA HongLing; LIU ChaoWu; GE Feng; XIAO ChuanLe; LU ChunHua; WANG Tong; HE QingYu

doi:10.3967/0895-3988.2011.04.014

Identification of Ubiquitinated Proteins from Human Multiple Myeloma U266 Cells by Proteomics

JIA HongLing, LIU ChaoWu, GE Feng, XIAO ChuanLe, LU ChunHua, WANG Tong, HE QingYu

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(4): 422-430

JIA HongLing, LIU ChaoWu, GE Feng, XIAO ChuanLe, LU ChunHua, WANG Tong, HE QingYu. Identification of Ubiquitinated Proteins from Human Multiple Myeloma U266 Cells by Proteomics[J]. Biomedical and Environmental Sciences, 2011, 24(4): 422-430. doi: 10.3967/0895-3988.2011.04.014

Citation:

JIA HongLing, LIU ChaoWu, GE Feng, XIAO ChuanLe, LU ChunHua, WANG Tong, HE QingYu. Identification of Ubiquitinated Proteins from Human Multiple Myeloma U266 Cells by Proteomics[J]. Biomedical and Environmental Sciences, 2011, 24(4): 422-430. doi: 10.3967/0895-3988.2011.04.014

doi: 10.3967/0895-3988.2011.04.014

基金项目: the 2007 Chang-Jiang Scholars Program,National Natural Science Foundation of China(30973393 & 30400071)%"211" Projects grant (Biotechnology & Bioengineering Medicine and Biomaterial & Tissue Engineering)

Identification of Ubiquitinated Proteins from Human Multiple Myeloma U266 Cells by Proteomics

Institute of Life and Health Engineering/National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, Guangdong, China
Guangdong Institute of Microbiology, Guangzhou 510070,Guangdong, China

Funds: the 2007 Chang-Jiang Scholars Program,National Natural Science Foundation of China(30973393 & 30400071)%"211" Projects grant (Biotechnology & Bioengineering Medicine and Biomaterial & Tissue Engineering)

摘要: Objective To identify ubiquitinated proteins from complex human multiple myeloma (MM) U266 cells,a malignant disorder of differentiated human B cells.Methods Employing a globally proteomic strategy combining of immunoprecipitation,LC-MS/MS and SCX-LC-MS analysis to identified ubiquitination sites,which were identified by detecting signature peptides containing a GG-tag (114.1 Da) and an LRGG-tag (383.2 Da).Results In total,52 ubiquitinated proteins containing 73 ubiquitination sites of which 14 and 59 sites contained LRGG-tag and GG-tag were identified,respectively.Conclusion Classification analysis by of the proteins identified in the study based on the PANTHER showed that they were associated with multiple functional groups.This suggested the involvement of many endogenous proteins in the ubiquitination in MM.
- /
- /
- /
Abstract: Objective To identify ubiquitinated proteins from complex human multiple myeloma (MM) U266 cells,a malignant disorder of differentiated human B cells.Methods Employing a globally proteomic strategy combining of immunoprecipitation,LC-MS/MS and SCX-LC-MS analysis to identified ubiquitination sites,which were identified by detecting signature peptides containing a GG-tag (114.1 Da) and an LRGG-tag (383.2 Da).Results In total,52 ubiquitinated proteins containing 73 ubiquitination sites of which 14 and 59 sites contained LRGG-tag and GG-tag were identified,respectively.Conclusion Classification analysis by of the proteins identified in the study based on the PANTHER showed that they were associated with multiple functional groups.This suggested the involvement of many endogenous proteins in the ubiquitination in MM.
- Ubiquitination /
- Multiple myeloma /
- Immunoprecipitation /
- Ubiquitinated proteins

[1]	Qiang Zhou, Meiyu Chang, Ning Li, Yi Guan, Sanqiao Yao. Antibiotic-Depleted Lung Microbiota Modulates Surfactant Proteins Expression and Reduces Experimental Silicosis . Biomedical and Environmental Sciences, 2025, 38(4): 469-483. doi: 10.3967/bes2025.012
[2]	JIANGTULU Bahabaike, LAN Chang Xin, CHEN Jun Xi, CHEN Xi, WANG Bin, XUE Tao. Ambient Fine Particulate Matter Exposure and Blood Pressure: Evidence from a Large Chinese Multiple Follow-Up Study . Biomedical and Environmental Sciences, 2023, 36(1): 38-49. doi: 10.3967/bes2023.004
[3]	DU Min, ZHANG Shi Mo, SHANG Wei Jing, YAN Wen Xin, LIU Qiao, QIN Chen Yuan, LIU Min, LIU Jue. 2022 Multiple-country Monkeypox Outbreak and Its Importation Risk into China: An Assessment Based on the Risk Matrix Method . Biomedical and Environmental Sciences, 2022, 35(10): 878-887. doi: 10.3967/bes2022.115
[4]	CAI Ying, LIU Yan Ping, ZHENG Kai, LEI Yuan Di, WANG Ye, XU Xin Yun, ZHANG Zhao Hui. Proteomics Study on the Differentially Expressed Proteins in 16HBE Cells Exposed to Beryllium Sulfate . Biomedical and Environmental Sciences, 2021, 34(11): 926-930. doi: 10.3967/bes2021.128
[5]	YE Cheng Yin, LI Jin Mei, WU Jing Hua, LI Zheng, XIAO Juan, YIN Xiao Yu, WANG Da Hui, XU Guo Zhang, YANG Lei. A Risk Prediction Model for Ischemic Stroke in Southern Chinese Population: Impact of Multiple Genetic Variants and Clinical/Lifestyle Factors . Biomedical and Environmental Sciences, 2021, 34(8): 641-645. doi: 10.3967/bes2021.089
[6]	XIAO Shi Qi, XU Da, DUAN Hong Yang, FAN Xue Ting, LI Gui Lian, ZHANG Wen, LI Ma Chao, HAN Na, LI Xin Yao, LI Na, ZHAO Li lan, ZHAO Xiu Qin, WAN Kang Lin, LIU Hai Can, FENG Wen Hai. Immunogenicity of Whole Mycobacterium intracellulare Proteins and Fingding on the Cross-Reactive Proteins between M. intracellulare and M. tuberculosis . Biomedical and Environmental Sciences, 2021, 34(7): 528-539. doi: 10.3967/bes2021.073
[7]	CAI Ying, ZHENG Kai, LI Run Bing, YU Shu Yuan, LIU Ning, JI Jia Jia, YANG Chen, WU De Sheng, QIN Shuang Jian, LI Bo Ru, ZHANG Zhao Hui, XU Xin Yun. Proteomics Study on the Differentially Expressed Proteins in c-fos-silenced Cells Exposed to PM_2.5 . Biomedical and Environmental Sciences, 2020, 33(9): 680-689. doi: 10.3967/bes2020.089
[8]	ZHENG Kai, CAI Ying, WANG Bing Yu, QIN Shuang Jian, LI Bo Ru, HUANG Hai Yan, QIN Xiao Yun, LONG Ding Xin, ZHANG Zhao Hui, XU Xin Yun. Characteristics of Atmospheric Fine Particulate Matter (PM_2.5) Induced Differentially Expressed Proteins Determined by Proteomics and Bioinformatics Analyses . Biomedical and Environmental Sciences, 2020, 33(8): 583-592. doi: 10.3967/bes2020.077
[9]	WANG Jin Zhang, YOU Li Bin, KAN Nai Peng, LIN Qi, WENG Yu Wei, ZHENG Kui Cheng. Frequent Import and Multiple Sources of Dengue Fever have Changed the Epidemic Situation of the Disease in Fujian Province, China . Biomedical and Environmental Sciences, 2020, 33(2): 123-132. doi: 10.3967/bes2020.016
[10]	Guesmi Fatma, Saidi Issam, Soussi Rawya, Hfaiedh Najla, Landoulsi Ahmed. Antioxidant Potential of Four Species of Natural Product and Therapeutic Strategies for Cancer through Suppression of Viability in the Human Multiple Myeloma Cell Line U266 . Biomedical and Environmental Sciences, 2019, 32(1): 22-33. doi: 10.3967/bes2019.003
[11]	LIU Wei, LIU Hui Xin, ZHANG Lin, HOU Xue Xia, WAN Kang Lin, HAO Qin. Evaluation of Six Recombinant Proteins for Serological Diagnosis of Lyme Borreliosis in China . Biomedical and Environmental Sciences, 2016, 29(5): 323-330. doi: 10.3967/bes2016.042
[12]	YUAN Wei Ming, LU Yuan Qiang, ZHENG Wei, CHEN Ming, HE Sheng Liang, MAI Wen Yuan, ZHANG Mei Bian. An Epistaxis Emergency Associated with Multiple Pollutants in Elementary Students . Biomedical and Environmental Sciences, 2016, 29(12): 893-897. doi: 10.3967/bes2016.119
[13]	BAN Hai Qun, LI Tao, SHEN Jin, LI Jin, PENG Pin Zhang, YE Heng Ping, ZHANG Liu Bo. Effects of Multiple Cleaning and Disinfection Interventions on Infectious Diseases in Children：A Group Randomized Trial in China . Biomedical and Environmental Sciences, 2015, 28(11): 779-787. doi: 10.3967/bes2015.109
[14]	XIA Jun, ZHAO Hai Zhou, LU Guang Hua. Effects of Selected Metal Oxide Nanoparticles on Multiple Biomarkers in Carassius auratus . Biomedical and Environmental Sciences, 2013, 26(9): 742-749. doi: 10.3967/0895-3988.2013.09.005
[15]	ZHEN-MING HU, SEAN A. F. PEEL, STEPHEN K. C. HO, GEORGE K. B. SANDOR, CAMERON M. L. CLOKIE. Induction of Bone Matrix Protein Expression by Native Bone Matrix Proteins in C2C12 Culture . Biomedical and Environmental Sciences, 2009, 22(2): 164-169.
[16]	MING-MING JIA, KUN-XUE HONG, JIAN-PING CHEN, HONG-WEI LIU, SHA LIU, XIAO-QING ZHANG, HONG-JING ZHAO, YI-MING SHAO. CTL Responses to Regulatory Proteins Tat and Rev in HIV-1 B'/C Virus-Infected Individuals . Biomedical and Environmental Sciences, 2008, 21(4): 314-318.
[17]	ARCHANA KUMARI, POONAM KAKKAR. Screening of Antioxidant Potential of Selected Barks of Indian Medicinal Plants by Multiple in vitro Assays LI . Biomedical and Environmental Sciences, 2008, 21(1): 24-29.
[18]	CHANG-QING YING, SONG-BIN FU, QUN XU, WEI-JUN TONG, MING-WU FANG, ZHENG-LAI WU, CHANG-CHUN QIU, YONG-HONG ZHANG. Multiple Risk Factor Clustering and Risk of Hypertension in the Mongolian Ethnic Population of China . Biomedical and Environmental Sciences, 2007, 20(5): 381-385.
[19]	XIN-LI XIAO, HUI-YING JIANG, JIN ZHANG, JUN HAN, KAI NIE, XIAO-BO ZHOU, YIN-XIA HUANG, LAN CHEN, WEI ZHOU, BAO-YUN ZHANG, YONG LIU, XIAO-PING DONG. Preparation of Monoclonal Antibodies Against Prion Proteins With Full-length Hamster PrP . Biomedical and Environmental Sciences, 2005, 18(4): 273-280.
[20]	MI-FANG LIANG, RUN-LEI DU, JING-ZHI LIU, CHUAN LI, QUAN-FU ZHANG, LU-LU HAN, JIAN-SHI YU, SHU-MIN DUAN, XIAO-FANG WANG, KONG-XING WU, ZHAO-HUI XIONG, QI JIN, DE-XIN LI. SARS Patients-derived Human Recombinant Antibodies to S and M Proteins Efficiently Neutralize SARS-Coronavirus Infectivity . Biomedical and Environmental Sciences, 2005, 18(6): 363-374.

点击查看大图

计量

文章访问数: 1366
HTML全文浏览量: 280
PDF下载量: 59
被引次数: 0

Identification of Ubiquitinated Proteins from Human Multiple Myeloma U266 Cells by Proteomics

doi: 10.3967/0895-3988.2011.04.014

刊出日期: 2011-08-20

关键词:

Ubiquitination /

Multiple myeloma /

Immunoprecipitation /

Ubiquitinated proteins

摘要: Objective To identify ubiquitinated proteins from complex human multiple myeloma (MM) U266 cells,a malignant disorder of differentiated human B cells.Methods Employing a globally proteomic strategy combining of immunoprecipitation,LC-MS/MS and SCX-LC-MS analysis to identified ubiquitination sites,which were identified by detecting signature peptides containing a GG-tag (114.1 Da) and an LRGG-tag (383.2 Da).Results In total,52 ubiquitinated proteins containing 73 ubiquitination sites of which 14 and 59 sites contained LRGG-tag and GG-tag were identified,respectively.Conclusion Classification analysis by of the proteins identified in the study based on the PANTHER showed that they were associated with multiple functional groups.This suggested the involvement of many endogenous proteins in the ubiquitination in MM.

English Abstract

Identification of Ubiquitinated Proteins from Human Multiple Myeloma U266 Cells by Proteomics

Institute of Life and Health Engineering/National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, Guangdong, China

Guangdong Institute of Microbiology, Guangzhou 510070,Guangdong, China

Publish Date: 2011-08-20

Keywords:

Abstract: Objective To identify ubiquitinated proteins from complex human multiple myeloma (MM) U266 cells,a malignant disorder of differentiated human B cells.Methods Employing a globally proteomic strategy combining of immunoprecipitation,LC-MS/MS and SCX-LC-MS analysis to identified ubiquitination sites,which were identified by detecting signature peptides containing a GG-tag (114.1 Da) and an LRGG-tag (383.2 Da).Results In total,52 ubiquitinated proteins containing 73 ubiquitination sites of which 14 and 59 sites contained LRGG-tag and GG-tag were identified,respectively.Conclusion Classification analysis by of the proteins identified in the study based on the PANTHER showed that they were associated with multiple functional groups.This suggested the involvement of many endogenous proteins in the ubiquitination in MM.

Citation:

JIA HongLing, LIU ChaoWu, GE Feng, XIAO ChuanLe, LU ChunHua, WANG Tong, HE QingYu. Identification of Ubiquitinated Proteins from Human Multiple Myeloma U266 Cells by Proteomics[J]. Biomedical and Environmental Sciences, 2011, 24(4): 422-430. doi: 10.3967/0895-3988.2011.04.014

全文HTML

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

Biomedical and Environmental Sciences ISSN 0895-3988 CN 11-2816 / Q

Publish Under the Auspices of Chinese Center for Disease Control and Prevention

Distributed by Chinese Center for Disease Control and Prevention

Quick Links

Supported by Beijing Renhe Information Technology Co. Ltd E-mail: info@rhhz.net

doi: 10.3967/0895-3988.2011.04.014

Identification of Ubiquitinated Proteins from Human Multiple Myeloma U266 Cells by Proteomics

计量

出版历程

Identification of Ubiquitinated Proteins from Human Multiple Myeloma U266 Cells by Proteomics

doi: 10.3967/0895-3988.2011.04.014

English Abstract

Identification of Ubiquitinated Proteins from Human Multiple Myeloma U266 Cells by Proteomics

Institute of Life and Health Engineering/National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, Guangdong, China Guangdong Institute of Microbiology, Guangzhou 510070,Guangdong, China

全文HTML

目录

Institute of Life and Health Engineering/National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, Guangdong, China

Guangdong Institute of Microbiology, Guangzhou 510070,Guangdong, China