LIU Wei; LIU Hui Xin; ZHANG Lin; HOU Xue Xia; WAN Kang Lin; HAO Qin

doi:10.3967/bes2016.042

Evaluation of Six Recombinant Proteins for Serological Diagnosis of Lyme Borreliosis in China

LIU Wei, LIU Hui Xin, ZHANG Lin, HOU Xue Xia, WAN Kang Lin, HAO Qin

文章导航 > Biomedical and Environmental Sciences > 2016 > 29(5): 323-330

LIU Wei, LIU Hui Xin, ZHANG Lin, HOU Xue Xia, WAN Kang Lin, HAO Qin. Evaluation of Six Recombinant Proteins for Serological Diagnosis of Lyme Borreliosis in China[J]. Biomedical and Environmental Sciences, 2016, 29(5): 323-330. doi: 10.3967/bes2016.042

Citation:

LIU Wei, LIU Hui Xin, ZHANG Lin, HOU Xue Xia, WAN Kang Lin, HAO Qin. Evaluation of Six Recombinant Proteins for Serological Diagnosis of Lyme Borreliosis in China[J]. Biomedical and Environmental Sciences, 2016, 29(5): 323-330. doi: 10.3967/bes2016.042

doi: 10.3967/bes2016.042

基金项目: This study was supported by the National Science and Technology Major Project (2013ZX10004-001)

Evaluation of Six Recombinant Proteins for Serological Diagnosis of Lyme Borreliosis in China

State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Funds: This study was supported by the National Science and Technology Major Project (2013ZX10004-001)

摘要: ObjectiveIn this study, we evaluated the diagnostic efficiency of six recombinant proteins for the serodiagnosis of Lyme borreliosis (LB) and screened out the appropriate antigens to support the production of a Chinese clinical ELISA (enzyme-linked immunosorbent assay) kit for LB.
MethodsSix recombinant antigens, Fla B.g, OspC B.a, OspC B.g, P39 B.g, P83 B.g, and VlsE B.a, were used for ELISA to detect serum antibodies in LB, syphilis, and healthy controls. The ELISA results were used to generate receiver operating characteristic (ROC) curves, and the sensitivity and specificity of each protein was evaluated. All recombinant proteins were evaluated and screened by using logistic regression models.
ResultsTwo IgG (VlsE and OspC B.g) and two IgM (OspC B.g and OspC B.a) antigens were left by the logistic regression model screened. VlsE had the highest specificity for syphilis samples in the IgG test (87.7%,P<0.05). OspC B.g had the highest diagnostic value in the IgM test (AUC=0.871). Interactive effects between OspC B.a and Fla B.g could reduce the specificity of the ELISA.
ConclusionThree recombinant antigens, OspC B.g, OspC B.a, and VlsE B.a, were useful for ELISAs of LB. Additionally, the interaction between OspC B.a and Fla B.g should be examined in future research.
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Abstract: ObjectiveIn this study, we evaluated the diagnostic efficiency of six recombinant proteins for the serodiagnosis of Lyme borreliosis (LB) and screened out the appropriate antigens to support the production of a Chinese clinical ELISA (enzyme-linked immunosorbent assay) kit for LB.
MethodsSix recombinant antigens, Fla B.g, OspC B.a, OspC B.g, P39 B.g, P83 B.g, and VlsE B.a, were used for ELISA to detect serum antibodies in LB, syphilis, and healthy controls. The ELISA results were used to generate receiver operating characteristic (ROC) curves, and the sensitivity and specificity of each protein was evaluated. All recombinant proteins were evaluated and screened by using logistic regression models.
ResultsTwo IgG (VlsE and OspC B.g) and two IgM (OspC B.g and OspC B.a) antigens were left by the logistic regression model screened. VlsE had the highest specificity for syphilis samples in the IgG test (87.7%,P<0.05). OspC B.g had the highest diagnostic value in the IgM test (AUC=0.871). Interactive effects between OspC B.a and Fla B.g could reduce the specificity of the ELISA.
ConclusionThree recombinant antigens, OspC B.g, OspC B.a, and VlsE B.a, were useful for ELISAs of LB. Additionally, the interaction between OspC B.a and Fla B.g should be examined in future research.
- Lyme borreliosis /
- Recombinant proteins /
- Serological diagnosis /
- Logistic regression /
- ROC Biomed Environ Sci,2016 /
- 29(5):323-330

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Evaluation of Six Recombinant Proteins for Serological Diagnosis of Lyme Borreliosis in China

doi: 10.3967/bes2016.042

基金项目: This study was supported by the National Science and Technology Major Project (2013ZX10004-001)

刊出日期: 2016-05-20

关键词:

Lyme borreliosis /

Recombinant proteins /

Serological diagnosis /

Logistic regression /

ROC Biomed Environ Sci,2016 /

29(5):323-330

摘要: ObjectiveIn this study, we evaluated the diagnostic efficiency of six recombinant proteins for the serodiagnosis of Lyme borreliosis (LB) and screened out the appropriate antigens to support the production of a Chinese clinical ELISA (enzyme-linked immunosorbent assay) kit for LB.
MethodsSix recombinant antigens, Fla B.g, OspC B.a, OspC B.g, P39 B.g, P83 B.g, and VlsE B.a, were used for ELISA to detect serum antibodies in LB, syphilis, and healthy controls. The ELISA results were used to generate receiver operating characteristic (ROC) curves, and the sensitivity and specificity of each protein was evaluated. All recombinant proteins were evaluated and screened by using logistic regression models.
ResultsTwo IgG (VlsE and OspC B.g) and two IgM (OspC B.g and OspC B.a) antigens were left by the logistic regression model screened. VlsE had the highest specificity for syphilis samples in the IgG test (87.7%,P<0.05). OspC B.g had the highest diagnostic value in the IgM test (AUC=0.871). Interactive effects between OspC B.a and Fla B.g could reduce the specificity of the ELISA.
ConclusionThree recombinant antigens, OspC B.g, OspC B.a, and VlsE B.a, were useful for ELISAs of LB. Additionally, the interaction between OspC B.a and Fla B.g should be examined in future research.

English Abstract

Evaluation of Six Recombinant Proteins for Serological Diagnosis of Lyme Borreliosis in China

State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Funds: This study was supported by the National Science and Technology Major Project (2013ZX10004-001)

Publish Date: 2016-05-20

Keywords:

Abstract: ObjectiveIn this study, we evaluated the diagnostic efficiency of six recombinant proteins for the serodiagnosis of Lyme borreliosis (LB) and screened out the appropriate antigens to support the production of a Chinese clinical ELISA (enzyme-linked immunosorbent assay) kit for LB.
MethodsSix recombinant antigens, Fla B.g, OspC B.a, OspC B.g, P39 B.g, P83 B.g, and VlsE B.a, were used for ELISA to detect serum antibodies in LB, syphilis, and healthy controls. The ELISA results were used to generate receiver operating characteristic (ROC) curves, and the sensitivity and specificity of each protein was evaluated. All recombinant proteins were evaluated and screened by using logistic regression models.
ResultsTwo IgG (VlsE and OspC B.g) and two IgM (OspC B.g and OspC B.a) antigens were left by the logistic regression model screened. VlsE had the highest specificity for syphilis samples in the IgG test (87.7%,P<0.05). OspC B.g had the highest diagnostic value in the IgM test (AUC=0.871). Interactive effects between OspC B.a and Fla B.g could reduce the specificity of the ELISA.
ConclusionThree recombinant antigens, OspC B.g, OspC B.a, and VlsE B.a, were useful for ELISAs of LB. Additionally, the interaction between OspC B.a and Fla B.g should be examined in future research.

Citation: