CHEN Bo; LIU Ying; SONG Wei Ming; HAYASHI Yasuhiko; DING Xun Cheng; LI Wei Hua

doi:10.3967/0895-3988.2011.06.002

In Vitro Evaluation of Cytotoxicity and Oxidative Stress Induced by Multiwalled Carbon Nanotubes in Murine RAW 264.7 Macrophages and Human A549 Lung Cells

CHEN Bo, LIU Ying, SONG Wei Ming, HAYASHI Yasuhiko, DING Xun Cheng, LI Wei Hua

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(6): 593-601

CHEN Bo, LIU Ying, SONG Wei Ming, HAYASHI Yasuhiko, DING Xun Cheng, LI Wei Hua. In Vitro Evaluation of Cytotoxicity and Oxidative Stress Induced by Multiwalled Carbon Nanotubes in Murine RAW 264.7 Macrophages and Human A549 Lung Cells[J]. Biomedical and Environmental Sciences, 2011, 24(6): 593-601. doi: 10.3967/0895-3988.2011.06.002

Citation:

CHEN Bo, LIU Ying, SONG Wei Ming, HAYASHI Yasuhiko, DING Xun Cheng, LI Wei Hua. In Vitro Evaluation of Cytotoxicity and Oxidative Stress Induced by Multiwalled Carbon Nanotubes in Murine RAW 264.7 Macrophages and Human A549 Lung Cells[J]. Biomedical and Environmental Sciences, 2011, 24(6): 593-601. doi: 10.3967/0895-3988.2011.06.002

doi: 10.3967/0895-3988.2011.06.002

基金项目: Shanghai Science and Technology Committee International Collaboration Program(055207078)

In Vitro Evaluation of Cytotoxicity and Oxidative Stress Induced by Multiwalled Carbon Nanotubes in Murine RAW 264.7 Macrophages and Human A549 Lung Cells

Key Laboratory of Public Health Safety, Ministry of Education, School of Public Health, Fudan University,Shanghai 200032, China
Putuo District Center for Disease Control and Prevention, Shanghai 200333, China
Graduate School of Engineering, Nagoya Institute of Technology, Nagoya 466-8555, Japan
NPFPC Key Laboratory of Contraceptive & Devices, Shanghai Institute of Planned Parenthood Research, Shanghai 200032,China

Funds: Shanghai Science and Technology Committee International Collaboration Program(055207078)

摘要: Objective To investigate in vitro cytotoxicity and oxidative stress response induced by multiwalled carbon nanotubes (MWCNTs).Methods Cultured macrophages (murine RAW264.7 cells) and alveolar epithelium cells type Ⅱ (human A549 lung cells) were exposed to the blank control,DNA salt control,and the MWCNTs suspensions at 2.5,10,25,and 100 μg/mL for 24 h.Each treatment was evaluated by cell viability,cytotoxicity and oxidative stress.Results Overall,both cell lines had similar patterns in response to the cytotoxicity and oxidative stress of MWCNTs.DNA salt treatment showed no change compared to the blank control.In both cell lines,significant changes at the doses of 25 and 100 μg/mL treatments were found in cell viabilities,cytotoxicity,and oxidative stress indexes.The reactive oxygen species (ROS) generation was also found to be significantly higher at the dose of 10 μg/mL treatment,whereas no change was seen in most of the indexes.The ROS generation in both cell lines went up in minutes,reached the climax within an hour and faded down after several hours.Conclusion Exposure to MWCNTs resulted in a dose-dependent cytotoxicity in cultured RAW264.7 cells and A549 cells,that was closely correlated to the increased oxidative stress.
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Abstract: Objective To investigate in vitro cytotoxicity and oxidative stress response induced by multiwalled carbon nanotubes (MWCNTs).Methods Cultured macrophages (murine RAW264.7 cells) and alveolar epithelium cells type Ⅱ (human A549 lung cells) were exposed to the blank control,DNA salt control,and the MWCNTs suspensions at 2.5,10,25,and 100 μg/mL for 24 h.Each treatment was evaluated by cell viability,cytotoxicity and oxidative stress.Results Overall,both cell lines had similar patterns in response to the cytotoxicity and oxidative stress of MWCNTs.DNA salt treatment showed no change compared to the blank control.In both cell lines,significant changes at the doses of 25 and 100 μg/mL treatments were found in cell viabilities,cytotoxicity,and oxidative stress indexes.The reactive oxygen species (ROS) generation was also found to be significantly higher at the dose of 10 μg/mL treatment,whereas no change was seen in most of the indexes.The ROS generation in both cell lines went up in minutes,reached the climax within an hour and faded down after several hours.Conclusion Exposure to MWCNTs resulted in a dose-dependent cytotoxicity in cultured RAW264.7 cells and A549 cells,that was closely correlated to the increased oxidative stress.
- Multi-wall carbon nanotubes /
- Cytotoxicity /
- Oxidative stress /
- RAW 264.7 cells /
- A549 cells

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In Vitro Evaluation of Cytotoxicity and Oxidative Stress Induced by Multiwalled Carbon Nanotubes in Murine RAW 264.7 Macrophages and Human A549 Lung Cells

doi: 10.3967/0895-3988.2011.06.002

基金项目: Shanghai Science and Technology Committee International Collaboration Program(055207078)

刊出日期: 2011-12-20

关键词:

Multi-wall carbon nanotubes /

摘要: Objective To investigate in vitro cytotoxicity and oxidative stress response induced by multiwalled carbon nanotubes (MWCNTs).Methods Cultured macrophages (murine RAW264.7 cells) and alveolar epithelium cells type Ⅱ (human A549 lung cells) were exposed to the blank control,DNA salt control,and the MWCNTs suspensions at 2.5,10,25,and 100 μg/mL for 24 h.Each treatment was evaluated by cell viability,cytotoxicity and oxidative stress.Results Overall,both cell lines had similar patterns in response to the cytotoxicity and oxidative stress of MWCNTs.DNA salt treatment showed no change compared to the blank control.In both cell lines,significant changes at the doses of 25 and 100 μg/mL treatments were found in cell viabilities,cytotoxicity,and oxidative stress indexes.The reactive oxygen species (ROS) generation was also found to be significantly higher at the dose of 10 μg/mL treatment,whereas no change was seen in most of the indexes.The ROS generation in both cell lines went up in minutes,reached the climax within an hour and faded down after several hours.Conclusion Exposure to MWCNTs resulted in a dose-dependent cytotoxicity in cultured RAW264.7 cells and A549 cells,that was closely correlated to the increased oxidative stress.

English Abstract

In Vitro Evaluation of Cytotoxicity and Oxidative Stress Induced by Multiwalled Carbon Nanotubes in Murine RAW 264.7 Macrophages and Human A549 Lung Cells

Key Laboratory of Public Health Safety, Ministry of Education, School of Public Health, Fudan University,Shanghai 200032, China

Putuo District Center for Disease Control and Prevention, Shanghai 200333, China

Graduate School of Engineering, Nagoya Institute of Technology, Nagoya 466-8555, Japan

NPFPC Key Laboratory of Contraceptive & Devices, Shanghai Institute of Planned Parenthood Research, Shanghai 200032,China

Funds: Shanghai Science and Technology Committee International Collaboration Program(055207078)

Publish Date: 2011-12-20

Keywords:

Abstract: Objective To investigate in vitro cytotoxicity and oxidative stress response induced by multiwalled carbon nanotubes (MWCNTs).Methods Cultured macrophages (murine RAW264.7 cells) and alveolar epithelium cells type Ⅱ (human A549 lung cells) were exposed to the blank control,DNA salt control,and the MWCNTs suspensions at 2.5,10,25,and 100 μg/mL for 24 h.Each treatment was evaluated by cell viability,cytotoxicity and oxidative stress.Results Overall,both cell lines had similar patterns in response to the cytotoxicity and oxidative stress of MWCNTs.DNA salt treatment showed no change compared to the blank control.In both cell lines,significant changes at the doses of 25 and 100 μg/mL treatments were found in cell viabilities,cytotoxicity,and oxidative stress indexes.The reactive oxygen species (ROS) generation was also found to be significantly higher at the dose of 10 μg/mL treatment,whereas no change was seen in most of the indexes.The ROS generation in both cell lines went up in minutes,reached the climax within an hour and faded down after several hours.Conclusion Exposure to MWCNTs resulted in a dose-dependent cytotoxicity in cultured RAW264.7 cells and A549 cells,that was closely correlated to the increased oxidative stress.

Citation:

CHEN Bo, LIU Ying, SONG Wei Ming, HAYASHI Yasuhiko, DING Xun Cheng, LI Wei Hua. In Vitro Evaluation of Cytotoxicity and Oxidative Stress Induced by Multiwalled Carbon Nanotubes in Murine RAW 264.7 Macrophages and Human A549 Lung Cells[J]. Biomedical and Environmental Sciences, 2011, 24(6): 593-601. doi: 10.3967/0895-3988.2011.06.002