SUN Ru Bao; XI Zhu Ge; YAN Jun; YANG Hong Lian

doi:10.3967/0895-3988.2012.05.001

Cytotoxicity and Apoptosis Induction in Human HepG2 Hepatoma Cells by Decabromodiphenyl Ethane

SUN Ru Bao, XI Zhu Ge, YAN Jun, YANG Hong Lian

文章导航 > Biomedical and Environmental Sciences > 2012 > 25(5): 495-501

SUN Ru Bao, XI Zhu Ge, YAN Jun, YANG Hong Lian. Cytotoxicity and Apoptosis Induction in Human HepG2 Hepatoma Cells by Decabromodiphenyl Ethane[J]. Biomedical and Environmental Sciences, 2012, 25(5): 495-501. doi: 10.3967/0895-3988.2012.05.001

Citation:

SUN Ru Bao, XI Zhu Ge, YAN Jun, YANG Hong Lian. Cytotoxicity and Apoptosis Induction in Human HepG2 Hepatoma Cells by Decabromodiphenyl Ethane[J]. Biomedical and Environmental Sciences, 2012, 25(5): 495-501. doi: 10.3967/0895-3988.2012.05.001

doi: 10.3967/0895-3988.2012.05.001

基金项目: the NSFC(20877102)%"973"project(2010CB933904)

Cytotoxicity and Apoptosis Induction in Human HepG2 Hepatoma Cells by Decabromodiphenyl Ethane

Tianjin Key Laboratory of Risk Assessment and Control for Environmental and Food Safety,Tianjin Institute of Health and Environmental Medicine,Tianjin 300050,China;Beijing Institute of Disease Control and Prevention,Beijing 100071,China
Tianjin Key Laboratory of Risk Assessment and Control for Environmental and Food Safety,Tianjin Institute of Health and Environmental Medicine,Tianjin 300050,China

Funds: the NSFC(20877102)%"973"project(2010CB933904)

摘要: Objective To investigate the toxic effects of decabromodiphenyl ethane (DBDPE), used as an alternative to decabromodiphenyl ether in vitro.Methods HepG2 cells were cultured in the presence of DBDPE at various concentrations (3.125‐100.0mg/L) for 24, 48, and 72 h respectively and the toxic effect of DBDPE was studied.Results As evaluated by the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide and lactate dehydrogenase assays and nuclear morphological changes, DBDPE inhibited HepG2 viability in a timeand dose‐dependent manner within a range of 12.5 mg/L to 100 mg/L and for 48 h and 72 h. Induction of apoptosis was detected at 12.5‐100 mg/L at 48 h and 72 h by propidium iodide staining, accompanied with overproduction of reactive oxygen species (ROS). Furthermore, N‐acetyl‐L‐cysteine, a widely used ROS scavenger, significantly reduced DBDPE‐induced ROS levels and increased HepG2 cells viability.Conclusion DBDPE has cytotoxic and anti‐proliferation effect and can induce apoptosis in which ROS plays an important role
- /
- /
- /
- /
Abstract: Objective To investigate the toxic effects of decabromodiphenyl ethane (DBDPE), used as an alternative to decabromodiphenyl ether in vitro.Methods HepG2 cells were cultured in the presence of DBDPE at various concentrations (3.125‐100.0mg/L) for 24, 48, and 72 h respectively and the toxic effect of DBDPE was studied.Results As evaluated by the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide and lactate dehydrogenase assays and nuclear morphological changes, DBDPE inhibited HepG2 viability in a timeand dose‐dependent manner within a range of 12.5 mg/L to 100 mg/L and for 48 h and 72 h. Induction of apoptosis was detected at 12.5‐100 mg/L at 48 h and 72 h by propidium iodide staining, accompanied with overproduction of reactive oxygen species (ROS). Furthermore, N‐acetyl‐L‐cysteine, a widely used ROS scavenger, significantly reduced DBDPE‐induced ROS levels and increased HepG2 cells viability.Conclusion DBDPE has cytotoxic and anti‐proliferation effect and can induce apoptosis in which ROS plays an important role
- Apoptosis /
- Cytotoxicity /
- Decabromodiphenyl ethane /
- Flame retardants /
- Reactive oxygen species

[1]	XU Yong Jie, HU Mei Ling, ZHOU Liang Hui, WANG Qi, ZHANG Xi Quan, LUO Qing Bin. Effect of HSPB9 on Apoptosis of DF-1 Cells . Biomedical and Environmental Sciences, 2019, 32(2): 107-120. doi: 10.3967/bes2019.015
[2]	SUN Ru Bao, SHANG Shuai, ZHANG Wei, LIN Ben Cheng, WANG Qiang, SHI Yun, XI Zhu Ge. Endocrine Disruption Activity of 30-day Dietary Exposure to Decabromodiphenyl Ethane in Balb/C Mouse . Biomedical and Environmental Sciences, 2018, 31(1): 12-22. doi: 10.3967/bes2018.002
[3]	ZHAO Li, LI Jing, HAO Yan Hui, GAO Ya Bing, WANG Shui Ming, ZHANG Jing, DONG Ji, ZHOU Hong Mei, LIU Shu Chen, PENG Rui Yun. Microwave-induced Apoptosis and Cytotoxicity of NK Cells through ERK1/2 Signaling . Biomedical and Environmental Sciences, 2017, 30(5): 323-332. doi: 10.3967/bes2017.043
[4]	YUAN Zhun, YING Xian Ping, ZHONG Wei Jian, TIAN Shi Min, WANG Yu, JIA Yong Rui, CHEN Wen, FU Juan Ling, ZHAO Peng, ZHOU Zong Can. Autophagy Attenuates MnCl2-induced Apoptosis in Human Bronchial Epithelial Cells . Biomedical and Environmental Sciences, 2016, 29(7): 494-504. doi: 10.3967/bes2016.065
[5]	TAO Yong Kang, YU Pu Lin, BAI Yong Ping, YAN Sheng Tao, ZHAO Shui Ping, ZHANG Guo Qiang. Role of PERK/eIF2α/CHOP Endoplasmic Reticulum Stress Pathway in Oxidized Low-density Lipoprotein Mediated Induction of Endothelial Apoptosis . Biomedical and Environmental Sciences, 2016, 29(12): 868-876. doi: 10.3967/bes2016.116
[6]	YUAN Yan, WANG Yi, HU Fei Fei, JIANG Chen Yang, ZHANG Ya Jing, YANG Jin Long, ZHAO Shi Wen, GU Jian Hong, LIU Xue Zhong, BIAN Jian Chun, LIU Zong Ping. Cadmium Activates Reactive Oxygen Species-dependent AKT/mTOR and Mitochondrial Apoptotic Pathways in Neuronal Cells . Biomedical and Environmental Sciences, 2016, 29(2): 117-126. doi: 10.3967/bes2016.013
[7]	GAO Hui, LI Ling Yu, ZHANG Man, ZHANG Quan. Inactivated Sendai Virus Induces Apoptosis Mediated by Reactive Oxygen Species in Murine Melanoma Cells . Biomedical and Environmental Sciences, 2016, 29(12): 877-884. doi: 10.3967/bes2016.117
[8]	ZHAO Ya Ning, LI Qiang. Adenovirus Mediated BIMS Transfer Induces Growth Supression and Apoptosis in Raji Lymphoma Cells . Biomedical and Environmental Sciences, 2014, 27(9): 655-664. doi: 10.3967/bes2014.101
[9]	JIA Hong Li, WANG Chao, LI Yue, LU Yan, WANG Ping Ping, PAN Wei Dong, SONG Tao. Combined Effects of 50 Hz Magnetic Field and Magnetic Nanoparticles on the Proliferation and Apoptosis of PC12 Cells . Biomedical and Environmental Sciences, 2014, 27(2): 97-106. doi: 10.3967/bes2014.022
[10]	WANG Lin, LIN Shu Qian, HE Yuan Long, LIU Gang, WANG Zhen Yong. Protective Effects of Quercetin on Cadmium-induced Cytotoxicity in Primary Cultures of Rat Proximal Tubular Cells . Biomedical and Environmental Sciences, 2013, 26(4): 258-267. doi: 10.3967/0895-3988.2013.04.004
[11]	WANG Jing, LV XiaoWen, SHI JiePing, HU XiaoSong, DU YuGuo. Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells . Biomedical and Environmental Sciences, 2011, 24(4): 391-399. doi: 10.3967/0895-3988.2011.04.010
[12]	CHEN Bo, LIU Ying, SONG Wei Ming, HAYASHI Yasuhiko, DING Xun Cheng, LI Wei Hua. In Vitro Evaluation of Cytotoxicity and Oxidative Stress Induced by Multiwalled Carbon Nanotubes in Murine RAW 264.7 Macrophages and Human A549 Lung Cells . Biomedical and Environmental Sciences, 2011, 24(6): 593-601. doi: 10.3967/0895-3988.2011.06.002
[13]	ARCHANA KUMARI, POONAM KAKKAR. Screening of Antioxidant Potential of Selected Barks of Indian Medicinal Plants by Multiple in vitro Assays LI . Biomedical and Environmental Sciences, 2008, 21(1): 24-29.
[14]	SHOU-LIANG GONG, LI-HUA DONG, GUANG-WEI LIU, PING-SHENG GONG, WEN-TIAN LU, HONG-GUANG ZHAO, XIAO-JING JIA, YONG ZHAO. Effects of Corticosterone,cAMP,cGMP,Ca2+,and Protein Kinase C on Apoptosis of Mouse Thymocytes Induced by X-ray Irradiation . Biomedical and Environmental Sciences, 2008, 21(2): 167-172.
[15]	XUE-WEN ZHANG, RONG-GUI LI, XIN WANG, SHUAN-HU ZHOU. Effects of Copper-phenanthroline on Pentschlorophenol-induced Adaptation and Cell Death of Escherichia coli . Biomedical and Environmental Sciences, 2007, 20(2): 106-112.
[16]	DIPANWITA DUTTA, S.SARAVANA DEVI, K.KRISHNAMURTHI, KOEL KUMAR, PRIYANKA VYAS, P.L.MUTHAL, P.NAOGHARE, T.CHAKRABARTI. Modulatory Effect of Distillate of Ocimum sanctum Leaf Extract(Tulsi)on Human Lymphocytes Against Genotoxicants . Biomedical and Environmental Sciences, 2007, 20(3): 226-234.
[17]	RI-AN YU, TAO XIA, AI-GUO WANG, XUE-MIN CHEN. Effects of Selenium and Zinc on Renal Oxidative Stress and Apoptosis Induced by Fluoride in Rats . Biomedical and Environmental Sciences, 2006, 19(6): 439-444.
[18]	YAN-QUN LIU, YI-KAI ZHOU. In vitro Cytotoxicity of TCDD on SPC-A1 Cells . Biomedical and Environmental Sciences, 2006, 19(1): 21-26.
[19]	AI-GUO WANG, TAO Xia, QI-LONG CHU, Ming Zhang, FANG LIU, XUE-MIN CHEN, KE-DI YANG. Effects of Fluoride on Lipid Peroxidation, DNA Damage and Apoptosis in Human Embryo Hepatocytes . Biomedical and Environmental Sciences, 2004, 17(2): 217-222.
[20]	XIE YI, ZHUANG ZHI_XIONG. Chromium(VI)-induced Production of Reactive Oxygen Species, Change of Plasma Membrane Potential and Dissipation of Mitochondria Membrane Potential in Chinese Hamster Lung Cell Cultures . Biomedical and Environmental Sciences, 2001, 14(3): 199-206.

点击查看大图

计量

文章访问数: 1536
HTML全文浏览量: 431
PDF下载量: 96
被引次数: 0

Cytotoxicity and Apoptosis Induction in Human HepG2 Hepatoma Cells by Decabromodiphenyl Ethane

doi: 10.3967/0895-3988.2012.05.001

基金项目: the NSFC(20877102)%"973"project(2010CB933904)

刊出日期: 2012-10-20

关键词:

Apoptosis /

Cytotoxicity /

Decabromodiphenyl ethane /

Flame retardants /

Reactive oxygen species

摘要: Objective To investigate the toxic effects of decabromodiphenyl ethane (DBDPE), used as an alternative to decabromodiphenyl ether in vitro.Methods HepG2 cells were cultured in the presence of DBDPE at various concentrations (3.125‐100.0mg/L) for 24, 48, and 72 h respectively and the toxic effect of DBDPE was studied.Results As evaluated by the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide and lactate dehydrogenase assays and nuclear morphological changes, DBDPE inhibited HepG2 viability in a timeand dose‐dependent manner within a range of 12.5 mg/L to 100 mg/L and for 48 h and 72 h. Induction of apoptosis was detected at 12.5‐100 mg/L at 48 h and 72 h by propidium iodide staining, accompanied with overproduction of reactive oxygen species (ROS). Furthermore, N‐acetyl‐L‐cysteine, a widely used ROS scavenger, significantly reduced DBDPE‐induced ROS levels and increased HepG2 cells viability.Conclusion DBDPE has cytotoxic and anti‐proliferation effect and can induce apoptosis in which ROS plays an important role

English Abstract

Cytotoxicity and Apoptosis Induction in Human HepG2 Hepatoma Cells by Decabromodiphenyl Ethane

Tianjin Key Laboratory of Risk Assessment and Control for Environmental and Food Safety,Tianjin Institute of Health and Environmental Medicine,Tianjin 300050,China;Beijing Institute of Disease Control and Prevention,Beijing 100071,China

Tianjin Key Laboratory of Risk Assessment and Control for Environmental and Food Safety,Tianjin Institute of Health and Environmental Medicine,Tianjin 300050,China

Funds: the NSFC(20877102)%"973"project(2010CB933904)

Publish Date: 2012-10-20

Keywords:

Abstract: Objective To investigate the toxic effects of decabromodiphenyl ethane (DBDPE), used as an alternative to decabromodiphenyl ether in vitro.Methods HepG2 cells were cultured in the presence of DBDPE at various concentrations (3.125‐100.0mg/L) for 24, 48, and 72 h respectively and the toxic effect of DBDPE was studied.Results As evaluated by the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide and lactate dehydrogenase assays and nuclear morphological changes, DBDPE inhibited HepG2 viability in a timeand dose‐dependent manner within a range of 12.5 mg/L to 100 mg/L and for 48 h and 72 h. Induction of apoptosis was detected at 12.5‐100 mg/L at 48 h and 72 h by propidium iodide staining, accompanied with overproduction of reactive oxygen species (ROS). Furthermore, N‐acetyl‐L‐cysteine, a widely used ROS scavenger, significantly reduced DBDPE‐induced ROS levels and increased HepG2 cells viability.Conclusion DBDPE has cytotoxic and anti‐proliferation effect and can induce apoptosis in which ROS plays an important role

Citation: