ZHAO Fei; CAO Bin; HE Li Hua; YIN Yu Dong; TAO Xiao Xia; SONG Shu Fan; MENG Fan Liang; ZHANG Jian Zhong

doi:10.3967/0895-3988.2012.01.011

Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens

ZHAO Fei, CAO Bin, HE Li Hua, YIN Yu Dong, TAO Xiao Xia, SONG Shu Fan, MENG Fan Liang, ZHANG Jian Zhong

文章导航 > Biomedical and Environmental Sciences > 2012 > 25(1): 77-81

ZHAO Fei, CAO Bin, HE Li Hua, YIN Yu Dong, TAO Xiao Xia, SONG Shu Fan, MENG Fan Liang, ZHANG Jian Zhong. Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens[J]. Biomedical and Environmental Sciences, 2012, 25(1): 77-81. doi: 10.3967/0895-3988.2012.01.011

Citation:

ZHAO Fei, CAO Bin, HE Li Hua, YIN Yu Dong, TAO Xiao Xia, SONG Shu Fan, MENG Fan Liang, ZHANG Jian Zhong. Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens[J]. Biomedical and Environmental Sciences, 2012, 25(1): 77-81. doi: 10.3967/0895-3988.2012.01.011

doi: 10.3967/0895-3988.2012.01.011

基金项目: This study was supported by the National Key Program for Infectious Diseases of China(2008ZX10004-002)

Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens

National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;State Key Laboratory for Infectious Disease Prevention and Control Beijing 102206, China
Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Beijing Institute of Respiratory Medicine, Capital Medical University, Beijing 100020, China;Beijing Key Laboratory of Respiratory and Pulmonary Circulation, Beijing 100020, China

Funds: This study was supported by the National Key Program for Infectious Diseases of China(2008ZX10004-002)

摘要: Objective To establish and evaluate a real-time PCR assay to detect Mycoplasma pneumoniae (M.pneumoniae) in clinical specimens.Methods By analysing the whole p1 gene sequence of 60 M.pneumoniae clinical isolates in Beijing of China,an optimized real-time PCR assay (MpP1) using p1 gene conserved region was designed.The specificity and sensitivity of this assay were evaluated and compared with other two reported assays (RepMp1 and Mp181) using 40 positive and 100 negative clinical specimens.Results The detection limit of the new assay was 8.1 fg (about 1～3CFU) M.pneumoniae DNA.The sensitivity of Mpp1,RepMp1,and Mp181 assays appeared to be 100％,100％,and 85％,respectively.Conclusion MpP1 assay is suitable for the detection of M.pneumoniae in Chinese clinical specimens.
- /
- /
Abstract: Objective To establish and evaluate a real-time PCR assay to detect Mycoplasma pneumoniae (M.pneumoniae) in clinical specimens.Methods By analysing the whole p1 gene sequence of 60 M.pneumoniae clinical isolates in Beijing of China,an optimized real-time PCR assay (MpP1) using p1 gene conserved region was designed.The specificity and sensitivity of this assay were evaluated and compared with other two reported assays (RepMp1 and Mp181) using 40 positive and 100 negative clinical specimens.Results The detection limit of the new assay was 8.1 fg (about 1～3CFU) M.pneumoniae DNA.The sensitivity of Mpp1,RepMp1,and Mp181 assays appeared to be 100％,100％,and 85％,respectively.Conclusion MpP1 assay is suitable for the detection of M.pneumoniae in Chinese clinical specimens.
- Mycoplasma pneumoniae /
- Real-time PCR /
- p1 gene

[1]	CHE Jie, CHEN Bo Han, XU Li, GAO Yuan, YUE Meng Meng, CHEN Zi Man, ZHANG Mao Jun, SHAO Zhu Jun. Establishment and Modification of Ninety-seven Pneumococcal Serotyping Assays Based on Quantitative Real-time Polymerase Chain Reaction . Biomedical and Environmental Sciences, 2023, 36(9): 787-799. doi: 10.3967/bes2023.078
[2]	YIN Cai Hong, SONG Zhan Yun, LIU Xing Xing, WANG Xiao Mu, WANG Ying, GAO Cheng Cheng, SONG Xiu Ling, FENG Xin. Comparison of Microdroplet Digital PCR Assays with Real-time Fluorescence Quantitative PCR for Clostridioides difficile Detection . Biomedical and Environmental Sciences, 2023, 36(7): 653-657. doi: 10.3967/bes2023.094
[3]	LIAO Lei, SHI Wen, MA Chao, TANG Wen Lian, QIAN Qi Lan, WANG Yu, LI Jiao Jiao, SHEN Jin Yang, JI Jing, MA Jin Ming, GAO Song. Duplex Detection of Vibrio Cholerae and Vibrio Parahaemolyticus by Real-time Recombinase Polymerase Amplification . Biomedical and Environmental Sciences, 2022, 35(12): 1161-1165. doi: 10.3967/bes2022.148
[4]	SHI Da Wei, WANG Dong Mei, NING Li Hua, LI Jing, DONG Yan, ZHANG Zhi Kun, DOU Hai Wei, WAN Rui Jie, JIA Chun Mei, XIN De LI. Using 16S rDNA Sequencing Technology to Preliminarily Analyze Intestinal Flora in Children with Mycoplasma pneumoniae Pneumonia . Biomedical and Environmental Sciences, 2022, 35(6): 528-537. doi: 10.3967/bes2022.070
[5]	WEI Xiao, LI Yan, LU Xin, ZHAO Rong Tao, YUAN Zheng Quan, SHI Hua, ZHAO Xiang Na. Rapid Detection of Yersinia pestis by Real-time Recombinase-aided Amplification . Biomedical and Environmental Sciences, 2021, 34(4): 309-313. doi: 10.3967/bes2021.040
[6]	XIAO Kang, YANG Xue Hua, ZOU Wen Quan, DONG Xiao Ping, SHI Qi. Assessment of the Sensitivity and Specificity of the Established Real-time Quaking-induced Conversion (RT-QuIC) Technique in Chinese CJD Surveillance . Biomedical and Environmental Sciences, 2020, 33(8): 620-622. doi: 10.3967/bes2020.081
[7]	DOU Hai Wei, TIAN Xiu Jun, XIN De Li, WEI Ran, ZHOU Wei, WANG Hong, QIN Xuan Guang, SHAO Jun Yan, XU Bao Ping, GE Li Xia, SHI Da Wei. Mycoplasma pneumoniae Macrolide Resistance and MLVA Typing in Children in Beijing, China, in 2016: Is It Relevant? . Biomedical and Environmental Sciences, 2020, 33(12): 916-924. doi: 10.3967/bes2020.125
[8]	CHE Jie, LU Jin Xing, LI Wen Ge, ZHANG Yun Fei, ZHAO Xiao Fei, YUAN Min, BAI Xue Mei, CHEN Xia, LI Juan. A New High-throughput Real-time PCR Assay for the Screening of Multiple Antimicrobial Resistance Genes in Broiler Fecal Samples from China . Biomedical and Environmental Sciences, 2019, 32(12): 881-892. doi: 10.3967/bes2019.111
[9]	WANG Hui, ZHANG Jing, HU Shao Hua, TAN Sheng Zhi, ZHANG Bo, ZHOU Hong Mei, PENG Rui Yun. Real-time Microwave Exposure Induces Calcium Efflux in Primary Hippocampal Neurons and Primary Cardiomyocytes . Biomedical and Environmental Sciences, 2018, 31(8): 561-571. doi: 10.3967/bes2018.077
[10]	SHAO Nan, LI Fan, NIE Kai, FU Shi Hong, ZHANG Wei Jia, HE Ying, LEI Wen Wen, WANG Qian Ying, LIANG Guo Dong, CAO Yu Xi, WANG Huan Yu. TaqMan Real-time RT-PCR Assay for Detecting and Differentiating Japanese Encephalitis Virus . Biomedical and Environmental Sciences, 2018, 31(3): 208-214. doi: 10.3967/bes2018.026
[11]	XIE Na, CHEN Zhao Yun, CHEN Tao, ZHU Bing Qing, XU Li, GAO Yuan, ZHANG Ai Yu, ZHAO Pan, LIU Ji Wen, SHAO Zhu Jun. A Cross-sectional Survey Assessing Carriage of Streptococcus pneumoniae in a Healthy Population in Xinjiang Uygur Autonomous Region of China . Biomedical and Environmental Sciences, 2018, 31(3): 233-237. doi: 10.3967/bes2018.029
[12]	XIAO Hong Li, XIN De Li, WANG Yan, CUI Li Jian, LIU Xiao Ya, LIU Song, SONG Li Hong, LIU Chun Ling, YIN Cheng Hong. Establishment of a Predictive Diagnostic Model for Acute Mycoplasma Pneumoniae Infection in Elderly Patients with Community-acquired Pneumonia . Biomedical and Environmental Sciences, 2017, 30(7): 540-544. doi: 10.3967/bes2017.072
[13]	LI Shao Li, SUN Hong Mei, ZHU Bao Li, LIU Fei, ZHAO Han Qing. Whole Genome Analysis Reveals New Insights into Macrolide Resistance in Mycoplasma pneumoniae . Biomedical and Environmental Sciences, 2017, 30(5): 343-350. doi: 10.3967/bes2017.045
[14]	CAO Yu Xi, HE Xiao Xia, FU Shi Hong, HE Ying, LI Hao, GAO Xiao Yan, LIANG Guo Dong. Real-time RT-PCR Assay for the Detection of Culex flavivirus . Biomedical and Environmental Sciences, 2015, 28(12): 917-919. doi: 10.3967/bes2015.019
[15]	LI Hao, CAO Yu Xi, HE Xiao Xia, FU Shi Hong, LYU Zhi, HE Ying, GAO Xiao Yan, LIANG Guo Dong, WANG Huan Yu. Real-time RT-PCR Assay for the detection of Tahyna Virus . Biomedical and Environmental Sciences, 2015, 28(5): 374-377. doi: 10.3967/bes2015.052
[16]	CHEN Xue Lan, ZHANG Bin, TANG Li, JIAO Hai Tao, XU Heng Yi, XU Feng, XU Hong, WEI Hua, XIONG Yong Hua. Expression and Characterization of ArgR, An Arginine Regulatory Protein in Corynebacterium crenatum . Biomedical and Environmental Sciences, 2014, 27(6): 436-443. doi: 10.3967/bes2014.072
[17]	NIU Pei Hua, ZHANG Chen, WANG Ji, TAN Wen Jie, MA Xue Jun. Detection and Identification of Six Foodborne Bacteria by Two-tube Multiplex Real Time PCR and Melting Curve Analysis . Biomedical and Environmental Sciences, 2014, 27(10): 770-778. doi: 10.3967/bes2014.112
[18]	LI Qiao, ZHANG Shu Hua, YU Yuan Hua, WANG Li Ping, GUAN Shu Wen, LI Peng Fei. Toxicity of Sodium Fluoride to Caenorhabditis elegans . Biomedical and Environmental Sciences, 2012, 25(2): 216-223. doi: 10.3967/0895-3988.2012.02.014
[19]	ZENG-HUI WU, YONG-LIANG LOU, YI-YU LU, JIE YAN. Development of Quantitative Real-time Polymerase Chain Reaction for the Detection of Vibrio vulnificus Based on Hemolysin (vvhA) Coding System . Biomedical and Environmental Sciences, 2008, 21(4): 296-301.
[20]	MA Qing-wen, LIN Guo-fang, CHEN JIG-ANG, SHEN JIAN-HUA. Polymorphism of Glutathione S-transferase T1, M1 and P1 Genes in a Shanghai Population: Patients With Occupational or Non-occupational Bladder Cancer . Biomedical and Environmental Sciences, 2002, 15(3): 253-260.

点击查看大图

计量

文章访问数: 1857
HTML全文浏览量: 580
PDF下载量: 200
被引次数: 0

Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens

doi: 10.3967/0895-3988.2012.01.011

基金项目: This study was supported by the National Key Program for Infectious Diseases of China(2008ZX10004-002)

刊出日期: 2012-02-20

关键词:

Mycoplasma pneumoniae /

Real-time PCR /

p1 gene

摘要: Objective To establish and evaluate a real-time PCR assay to detect Mycoplasma pneumoniae (M.pneumoniae) in clinical specimens.Methods By analysing the whole p1 gene sequence of 60 M.pneumoniae clinical isolates in Beijing of China,an optimized real-time PCR assay (MpP1) using p1 gene conserved region was designed.The specificity and sensitivity of this assay were evaluated and compared with other two reported assays (RepMp1 and Mp181) using 40 positive and 100 negative clinical specimens.Results The detection limit of the new assay was 8.1 fg (about 1～3CFU) M.pneumoniae DNA.The sensitivity of Mpp1,RepMp1,and Mp181 assays appeared to be 100％,100％,and 85％,respectively.Conclusion MpP1 assay is suitable for the detection of M.pneumoniae in Chinese clinical specimens.

English Abstract

Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens

National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;State Key Laboratory for Infectious Disease Prevention and Control Beijing 102206, China

Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Beijing Institute of Respiratory Medicine, Capital Medical University, Beijing 100020, China;Beijing Key Laboratory of Respiratory and Pulmonary Circulation, Beijing 100020, China

Funds: This study was supported by the National Key Program for Infectious Diseases of China(2008ZX10004-002)

Publish Date: 2012-02-20

Keywords:

Abstract: Objective To establish and evaluate a real-time PCR assay to detect Mycoplasma pneumoniae (M.pneumoniae) in clinical specimens.Methods By analysing the whole p1 gene sequence of 60 M.pneumoniae clinical isolates in Beijing of China,an optimized real-time PCR assay (MpP1) using p1 gene conserved region was designed.The specificity and sensitivity of this assay were evaluated and compared with other two reported assays (RepMp1 and Mp181) using 40 positive and 100 negative clinical specimens.Results The detection limit of the new assay was 8.1 fg (about 1～3CFU) M.pneumoniae DNA.The sensitivity of Mpp1,RepMp1,and Mp181 assays appeared to be 100％,100％,and 85％,respectively.Conclusion MpP1 assay is suitable for the detection of M.pneumoniae in Chinese clinical specimens.

Citation:

ZHAO Fei, CAO Bin, HE Li Hua, YIN Yu Dong, TAO Xiao Xia, SONG Shu Fan, MENG Fan Liang, ZHANG Jian Zhong. Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens[J]. Biomedical and Environmental Sciences, 2012, 25(1): 77-81. doi: 10.3967/0895-3988.2012.01.011