YAO Feng; LU Yuan Qiang; ZHANG Qin; JIANG Jiu Kun; YANG Yun Mei

doi:10.3967/0895-3988.2013.07.017

Establishment of Multiplex PCR for Simultaneous Detection of Four Venereal Pathogens

YAO Feng, LU Yuan Qiang, ZHANG Qin, JIANG Jiu Kun, YANG Yun Mei

文章导航 > Biomedical and Environmental Sciences > 2013 > 26(7): 622-624

YAO Feng, LU Yuan Qiang, ZHANG Qin, JIANG Jiu Kun, YANG Yun Mei. Establishment of Multiplex PCR for Simultaneous Detection of Four Venereal Pathogens[J]. Biomedical and Environmental Sciences, 2013, 26(7): 622-624. doi: 10.3967/0895-3988.2013.07.017

Citation:

YAO Feng, LU Yuan Qiang, ZHANG Qin, JIANG Jiu Kun, YANG Yun Mei. Establishment of Multiplex PCR for Simultaneous Detection of Four Venereal Pathogens[J]. Biomedical and Environmental Sciences, 2013, 26(7): 622-624. doi: 10.3967/0895-3988.2013.07.017

doi: 10.3967/0895-3988.2013.07.017

Establishment of Multiplex PCR for Simultaneous Detection of Four Venereal Pathogens

Department of Emergency and Geriatrics Medicine, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, China

摘要: Venereal diseases are considered to be the most prevalent infectious diseases in the worldwide.China is now faced with a year-by-year increasing incidence of sexually transmitted diseases (STD),which are spreading from high-risk groups to the general population.Neisseria gonorrhoeae,Chlamydia trachomatis,Ureaplasma urealyticum and herpes simplex virus-2 (HSV-2) are always regarded as the most common venereal pathogens.The "golden standard" for testing Neisseria gonorrhoeae remains to be bacteria culture or microscopic examination.The sensitivity of these testing methods,however,is seriously influenced by some factors.High-quality specimen as well as fast inspection is required considering the poor viability of Neisseria gonorrhoeae in vitro.Furthermore,misuse and overuse of antibiotics can also contribute to atypical symptoms which also reduce the sensitivity of conventional methods mentioned above.Thus,common PCR and fluorogenic quantitative PCR have played the role of routine screening methods[1-2].
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- Neisseria gonorrhoeae /
- sexually transmitted diseases /
- infectious diseases /
- testing methods /
- in vitro

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Establishment of Multiplex PCR for Simultaneous Detection of Four Venereal Pathogens

doi: 10.3967/0895-3988.2013.07.017

刊出日期: 2013-07-20

关键词:

Neisseria gonorrhoeae /

sexually transmitted diseases /

infectious diseases /

testing methods /

in vitro

摘要: Venereal diseases are considered to be the most prevalent infectious diseases in the worldwide.China is now faced with a year-by-year increasing incidence of sexually transmitted diseases (STD),which are spreading from high-risk groups to the general population.Neisseria gonorrhoeae,Chlamydia trachomatis,Ureaplasma urealyticum and herpes simplex virus-2 (HSV-2) are always regarded as the most common venereal pathogens.The "golden standard" for testing Neisseria gonorrhoeae remains to be bacteria culture or microscopic examination.The sensitivity of these testing methods,however,is seriously influenced by some factors.High-quality specimen as well as fast inspection is required considering the poor viability of Neisseria gonorrhoeae in vitro.Furthermore,misuse and overuse of antibiotics can also contribute to atypical symptoms which also reduce the sensitivity of conventional methods mentioned above.Thus,common PCR and fluorogenic quantitative PCR have played the role of routine screening methods[1-2].