FANG Jin; LIANG Chun Lai; JIA Xu Dong; LI Ning

doi:10.3967/bes2014.069

Immunotoxicity of Acrylamide in Female BALB/c Mice

FANG Jin, LIANG Chun Lai, JIA Xu Dong, LI Ning

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(6): 401-409

FANG Jin, LIANG Chun Lai, JIA Xu Dong, LI Ning. Immunotoxicity of Acrylamide in Female BALB/c Mice[J]. Biomedical and Environmental Sciences, 2014, 27(6): 401-409. doi: 10.3967/bes2014.069

Citation:

FANG Jin, LIANG Chun Lai, JIA Xu Dong, LI Ning. Immunotoxicity of Acrylamide in Female BALB/c Mice[J]. Biomedical and Environmental Sciences, 2014, 27(6): 401-409. doi: 10.3967/bes2014.069

doi: 10.3967/bes2014.069

基金项目: ?This research was supported by the National Science and Technology Support Program (2012BAK01B00)

Immunotoxicity of Acrylamide in Female BALB/c Mice

Key Laboratory of Food Safety Risk Assessment, Ministry of Health, National Center for Food Safety Risk Assessment, Beijing 100021, China

Funds: ?This research was supported by the National Science and Technology Support Program (2012BAK01B00)

摘要: Objective To investigate the immunotoxicity of acrylamide (ACR) in female BALB/c mice.
Methods A total of 200 female mice weighing 18-22 g were randomly divided into four clusters based on body weight, and each weight-based cluster included five groups (10 mice per group): negative control, positive control (cyclophosphamide), low, intermediate, and high dose ACR groups, and all the groups were administered ACR by gavage for 30 days. At the end of the study, the immunotoxicological effects of the ACR were evaluated through immunopathology, humoral immunity, cellular immunity, and non-specific immunity.
Results The terminal body weight, spleen and thymus weights, lymphocyte counts in the ACR-H group were decreased, pathological changes were observed in lymph glands, thymus and spleen.%T cells in blood lymphocytes were significantly increased in all ACR-treated groups, and a significant reduction of% natural killer(NK) cells and increase of %Th cells were observed in the ACR-H group. interleukin-6(IL-6), Concanavalin A(ConA)-induced splenocyte proliferation and serum half hemolysis value (HC50) were also significantly suppressed in the ACR-H group.
Conclusion ACR elicited an inhibitory effect on cellular and humoral immunity of mice after 30 day feeding.
- /
- /
Abstract: Objective To investigate the immunotoxicity of acrylamide (ACR) in female BALB/c mice.
Methods A total of 200 female mice weighing 18-22 g were randomly divided into four clusters based on body weight, and each weight-based cluster included five groups (10 mice per group): negative control, positive control (cyclophosphamide), low, intermediate, and high dose ACR groups, and all the groups were administered ACR by gavage for 30 days. At the end of the study, the immunotoxicological effects of the ACR were evaluated through immunopathology, humoral immunity, cellular immunity, and non-specific immunity.
Results The terminal body weight, spleen and thymus weights, lymphocyte counts in the ACR-H group were decreased, pathological changes were observed in lymph glands, thymus and spleen.%T cells in blood lymphocytes were significantly increased in all ACR-treated groups, and a significant reduction of% natural killer(NK) cells and increase of %Th cells were observed in the ACR-H group. interleukin-6(IL-6), Concanavalin A(ConA)-induced splenocyte proliferation and serum half hemolysis value (HC50) were also significantly suppressed in the ACR-H group.
Conclusion ACR elicited an inhibitory effect on cellular and humoral immunity of mice after 30 day feeding.
- Acrylamide(ACR) /
- Immunotoxicity /
- Female BALB/c mice

[1]	Lei Li, Lin Luo, Junnai Wang, Ying Hong, Jianfang Geng. Multivariate Analysis of Female Stress Urinary Incontinence and Establishment of a Prediction Model . Biomedical and Environmental Sciences, 2024, 37(8): 931-935. doi: 10.3967/bes2024.114
[2]	MENG Shuang, MIAO Bei Bei, LI Jie, YIN Jian Wen, LIU Ze Liang, JIANG Xue Qi, GONG Xin Yi, LI Juan. Isolation of Leclercia adecarboxylata Producing Carbapenemases in A Newborn Female . Biomedical and Environmental Sciences, 2023, 36(9): 874-879. doi: 10.3967/bes2023.104
[3]	ZHANG Xin Sheng, ZHANG Peng, LIU Ying Hua, XU Qing, ZHANG Yong, LI Hui Zi, LIU Lu, LIU Yu Meng, YANG Xue Yan, XUE Chang Yong. Caprylic Acid Improves Lipid Metabolism, Suppresses the Inflammatory Response and Activates the ABCA1/p-JAK2/p-STAT3 Signaling Pathway in C57BL/6J Mice and RAW264.7 Cells . Biomedical and Environmental Sciences, 2022, 35(2): 95-106. doi: 10.3967/bes2022.014
[4]	LI Xiao Hong, YU Xiao Li, ZHOU Jian, SHAN Hui, OUYANG Chuan, KE Hong Yang, WU Jin Tao, LI Wan Wei. Induction of Lung Injury by Cooking Oil Fumes in Mice and the Protective Effects of Vitamin C . Biomedical and Environmental Sciences, 2021, 34(11): 916-919. doi: 10.3967/bes2021.126
[5]	LIU Yang, LI You Zhi, FENG Tao, GONG Pi Qian, JIN Ying Cai, ZHU Hong Wei, JIANG Lin Lin, ZHANG Jian Long, CHEN Guo Zhong, YU Xin, ZHANG Xing Xiao. Virulence of Salmonella enterica Serovar Enteritidis Isolate LN-248-0 and Immune Responses in BALB/c Mice . Biomedical and Environmental Sciences, 2020, 33(8): 628-632. doi: 10.3967/bes2020.083
[6]	Aline Parisoto Missio, Thais Fernanda de Campos Fraga-Silva, Larissa Lumi Watanabe Ishikawa, Luiza Ayumi Nishiyama Mimura, Thais Graziela Donegá França, Larissa Ragozo Cardoso de Oliveira, Alexandrina Sartori, Sofia Fernanda Gonçalves Zorzella-Pezavento. C57BL/6 and DBA/1 Mice Differ in Their Response to Supplementation with 1, 25D and Paricalcitol . Biomedical and Environmental Sciences, 2018, 31(8): 613-618. doi: 10.3967/bes2018.083
[7]	SUN Ru Bao, SHANG Shuai, ZHANG Wei, LIN Ben Cheng, WANG Qiang, SHI Yun, XI Zhu Ge. Endocrine Disruption Activity of 30-day Dietary Exposure to Decabromodiphenyl Ethane in Balb/C Mouse . Biomedical and Environmental Sciences, 2018, 31(1): 12-22. doi: 10.3967/bes2018.002
[8]	ZHANG Bin, SHAO Hua, WANG Xiu Hui, CHEN Xiao, LI Zhong Sheng, CAO Peng, ZHU Dan, YANG Yi Guang, XIAO Jing Wei, LI Bin. Acrylamide-induced Subacute Neurotoxic Effects on the Cerebral Cortex and Cerebellum at the Synapse Level in Rats . Biomedical and Environmental Sciences, 2017, 30(6): 432-443. doi: 10.3967/bes2017.057
[9]	ZHANG Yong, XU Qing, LIU Ying Hua, ZHANG Xin Sheng, WANG Jin, YU Xiao Ming, ZHANG Rong Xin, XUE Chao, YANG Xue Yan, XUE Chang Yong. Medium-Chain Triglyceride Activated Brown Adipose Tissue and Induced Reduction of Fat Mass in C57BL/6J Mice Fed High-fat Diet . Biomedical and Environmental Sciences, 2015, 28(2): 97-104. doi: 10.3967/bes2015.012
[10]	YU Su Fang, WEI Xiao Min, YAN Feng Feng, WANG Shu E, ZHANG Cui Li, YANG Xi Wei. The Role of NF mRNA and Calpain in NF Reduction of Acrylamide Neuropathy . Biomedical and Environmental Sciences, 2015, 28(6): 445-448. doi: 10.3967/bes2015.062
[11]	Amany A.Abdin, Dalia S.Ashour, Zeinab S.Shoheib. Artesunate Effect on Schistosome Thioredoxin Glutathione Reductase and Cytochrome c Peroxidase as New Molecular Targets in Schistosoma mansoni-infected Mice . Biomedical and Environmental Sciences, 2013, 26(12): 953-961. doi: 10.3967/bes2013.030
[12]	YU Su Fang, SONG Fu Ying, YI Chao, YANG Xi Wei, LI Guo Zhen, ZHANG Cui Li, ZHAO Xiu Lan, XIE Ke Qin. Acrylamide Alters Cytoskeletal Protein Level in Rat Serum . Biomedical and Environmental Sciences, 2013, 26(11): 926-929. doi: 10.3967/bes2013.023
[13]	ZHOU Ping Ping, ZHAO Yun Feng, LIU Hua Liang, MA Yong Jian, LI Xiao Wei, YANG Xin, WU Yong Ning. Dietary Exposure of the Chinese Population to Acrylamide . Biomedical and Environmental Sciences, 2013, 26(6): 421-429. doi: 10.3967/0895-3988.2013.06.002
[14]	LIANG Chun Lai, ZHANG Xiao Peng, SONG Yan, JIA Xu Dong. Immunotoxicological Evaluation of Wheat Genetically Modified with TaDREB4 Gene on BALB/c Mice . Biomedical and Environmental Sciences, 2013, 26(8): 663-670. doi: 10.3967/0895-3988.2013.08.005
[15]	SHI Yao Fang, SHAO Xin Yu, LOU Qing Qing, CHEN Ya Juan, ZHOU Hui Juan, ZOU Jian Ying. Study on Female Sexual Dysfunction in Type 2 Diabetic Chinese Women . Biomedical and Environmental Sciences, 2012, 25(5): 557-561. doi: 10.3967/0895-3988.2012.05.009
[16]	LIU Jin, HUANG Hui Ling, PANG Fen, ZHANG Wen Chang. The Effect of n-Hexane on the Gonad Toxicity of Female Mice . Biomedical and Environmental Sciences, 2012, 25(2): 189-196. doi: 10.3967/0895-3988.2012.02.010
[17]	MIN YOUNG KIM, M YUNG CHO. Toxicity and Carcinogenicity of Ozone in Combination with 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and Dibutyl Phthalate in B6C3F1 Mice for 16 and 32 Weeks . Biomedical and Environmental Sciences, 2009, 22(3): 216-222.
[18]	WEI-WEI LI, HUI LI, ZHI-FEI LIU, QUN QIAO. Determination of Residual Acrylamide in Medical Polyacrylamide Hydrogel by High Performance Liquid Chromatography tandem Mass Spectroscopy . Biomedical and Environmental Sciences, 2009, 22(1): 28-31.
[19]	WEI HE, ZHEN-YU JI, CHENG-YU HUANG. Molecular Cloning of a Novel cDNA From Mus Muscular BALB/c Mice Encoding Glycosyl Hydrolase Family 1: A Homolog of HumanLactase-Phlorizin Hydrolase . Biomedical and Environmental Sciences, 2006, 19(5): 340-345.
[20]	YAO HONG-YAN, WANG ZENG-ZHEN, JIANG DONG-LIN, SUN jia-fu, NIU ZHONG-XIAN. Evaluation of the Effect of Interventions for the Female Drug Abusers . Biomedical and Environmental Sciences, 2002, 15(4): 341-346.

点击查看大图

计量

文章访问数: 2131
HTML全文浏览量: 738
PDF下载量: 76
被引次数: 0

Immunotoxicity of Acrylamide in Female BALB/c Mice

doi: 10.3967/bes2014.069

基金项目: ?This research was supported by the National Science and Technology Support Program (2012BAK01B00)

刊出日期: 2014-06-20

关键词:

Acrylamide(ACR) /

Immunotoxicity /

Female BALB/c mice

摘要: Objective To investigate the immunotoxicity of acrylamide (ACR) in female BALB/c mice.
Methods A total of 200 female mice weighing 18-22 g were randomly divided into four clusters based on body weight, and each weight-based cluster included five groups (10 mice per group): negative control, positive control (cyclophosphamide), low, intermediate, and high dose ACR groups, and all the groups were administered ACR by gavage for 30 days. At the end of the study, the immunotoxicological effects of the ACR were evaluated through immunopathology, humoral immunity, cellular immunity, and non-specific immunity.
Results The terminal body weight, spleen and thymus weights, lymphocyte counts in the ACR-H group were decreased, pathological changes were observed in lymph glands, thymus and spleen.%T cells in blood lymphocytes were significantly increased in all ACR-treated groups, and a significant reduction of% natural killer(NK) cells and increase of %Th cells were observed in the ACR-H group. interleukin-6(IL-6), Concanavalin A(ConA)-induced splenocyte proliferation and serum half hemolysis value (HC50) were also significantly suppressed in the ACR-H group.
Conclusion ACR elicited an inhibitory effect on cellular and humoral immunity of mice after 30 day feeding.

English Abstract

Immunotoxicity of Acrylamide in Female BALB/c Mice

Key Laboratory of Food Safety Risk Assessment, Ministry of Health, National Center for Food Safety Risk Assessment, Beijing 100021, China

Funds: ?This research was supported by the National Science and Technology Support Program (2012BAK01B00)

Publish Date: 2014-06-20

Keywords:

Abstract: Objective To investigate the immunotoxicity of acrylamide (ACR) in female BALB/c mice.
Methods A total of 200 female mice weighing 18-22 g were randomly divided into four clusters based on body weight, and each weight-based cluster included five groups (10 mice per group): negative control, positive control (cyclophosphamide), low, intermediate, and high dose ACR groups, and all the groups were administered ACR by gavage for 30 days. At the end of the study, the immunotoxicological effects of the ACR were evaluated through immunopathology, humoral immunity, cellular immunity, and non-specific immunity.
Results The terminal body weight, spleen and thymus weights, lymphocyte counts in the ACR-H group were decreased, pathological changes were observed in lymph glands, thymus and spleen.%T cells in blood lymphocytes were significantly increased in all ACR-treated groups, and a significant reduction of% natural killer(NK) cells and increase of %Th cells were observed in the ACR-H group. interleukin-6(IL-6), Concanavalin A(ConA)-induced splenocyte proliferation and serum half hemolysis value (HC50) were also significantly suppressed in the ACR-H group.
Conclusion ACR elicited an inhibitory effect on cellular and humoral immunity of mice after 30 day feeding.

FANG Jin, LIANG Chun Lai, JIA Xu Dong, LI Ning. Immunotoxicity of Acrylamide in Female BALB/c Mice[J]. Biomedical and Environmental Sciences, 2014, 27(6): 401-409. doi: 10.3967/bes2014.069

Citation:

FANG Jin, LIANG Chun Lai, JIA Xu Dong, LI Ning. Immunotoxicity of Acrylamide in Female BALB/c Mice[J]. Biomedical and Environmental Sciences, 2014, 27(6): 401-409. doi: 10.3967/bes2014.069