Hanen BOUAZIZ-KETATA; Ghada BEN SALAH; Hichem BEN SALAH; Rim MARREKCHI; Kamel JAMOUSSI; Tahia BOUDAWARA; Faiza FAKHFEKH; Najiba ZEGHAL

doi:10.3967/bes2014.105

Nitrate-induced Biochemical and Histopathological Changes in the Liver of Rats:Ameliorative Effect of Hyparrhenia hirta

Hanen BOUAZIZ-KETATA, Ghada BEN SALAH, Hichem BEN SALAH, Rim MARREKCHI, Kamel JAMOUSSI, Tahia BOUDAWARA, Faiza FAKHFEKH, Najiba ZEGHAL

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(9): 695-706

Hanen BOUAZIZ-KETATA, Ghada BEN SALAH, Hichem BEN SALAH, Rim MARREKCHI, Kamel JAMOUSSI, Tahia BOUDAWARA, Faiza FAKHFEKH, Najiba ZEGHAL. Nitrate-induced Biochemical and Histopathological Changes in the Liver of Rats:Ameliorative Effect of Hyparrhenia hirta[J]. Biomedical and Environmental Sciences, 2014, 27(9): 695-706. doi: 10.3967/bes2014.105

Citation:

Hanen BOUAZIZ-KETATA, Ghada BEN SALAH, Hichem BEN SALAH, Rim MARREKCHI, Kamel JAMOUSSI, Tahia BOUDAWARA, Faiza FAKHFEKH, Najiba ZEGHAL. Nitrate-induced Biochemical and Histopathological Changes in the Liver of Rats:Ameliorative Effect of Hyparrhenia hirta[J]. Biomedical and Environmental Sciences, 2014, 27(9): 695-706. doi: 10.3967/bes2014.105

doi: 10.3967/bes2014.105

基金项目: the ministry of higher education and scientific research in Tunisia (UR/11 ES70)

Nitrate-induced Biochemical and Histopathological Changes in the Liver of Rats:Ameliorative Effect of Hyparrhenia hirta

Animal Physiology Laboratory, UR/11 ES70, Sfax Faculty of Sciences, University of Sfax, BP1171, Sfax 3000, Tunisia
Laboratory of Human Molecular Genetics, Faculty of Medicine, Sfax 3029, Tunisia
Laboratory of Chemistry, Sfax Faculty of Sciences, University of Sfax, BP1171, Sfax 3000, Tunisia
Laboratory of Biochemistry, CHU Hedi Chaker, Sfax 3029, Tunisia；5. Histopathology Laboratory, University of Sfax, CHU Habib Bourguiba, Sfax 3029, Tunisia

Funds: the ministry of higher education and scientific research in Tunisia (UR/11 ES70)

摘要: Objective The present study investigated the protective role of Hyparrhenia hirta (H. hirta) against sodium nitrate (NaNO3)-induced hepatoxicity. Methods Male Wistar rats were randomly divided into three groups:a control group and two treated groups during 50 d with NaNO3 administered either alone in drinking water or co-administered with H. hirta. Results NaNO3 treatment induced a significant increase in serum levels of glucose, total cholesterol and triglyceride while serum total protein level decreased significantly. Transaminases and lactate deshydrogenase activities in serum were elevated indicating hepatic cells’ damage after treatment with NaNO3. The hyperbilirubinemia and the increased serum gamma glutamyl transferase activities suggested the presence of cholestasis in NaNO3 exposed rats. In parallel, a significant increase in malondialdehyde level along with a concomitant decrease in total glutathione content and superoxide dismutase, catalase and glutathione peroxidase activities were observed in the liver after NaNO3 treatment. Furthermore, nitrate caused a significant induction of DNA fragmentation. These modifications in NaNO3-treated rats corresponded histologically with hepatocellular necrosis and mononuclear cells infiltration. H. hirta supplementation showed a remarkable amelioration of the abnormalities cited above. Conclusion The results concluded that the treatment with H. hirta had a significant role in protecting the animals from nitrate-induced liver dysfunction.
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Abstract: Objective The present study investigated the protective role of Hyparrhenia hirta (H. hirta) against sodium nitrate (NaNO3)-induced hepatoxicity. Methods Male Wistar rats were randomly divided into three groups:a control group and two treated groups during 50 d with NaNO3 administered either alone in drinking water or co-administered with H. hirta. Results NaNO3 treatment induced a significant increase in serum levels of glucose, total cholesterol and triglyceride while serum total protein level decreased significantly. Transaminases and lactate deshydrogenase activities in serum were elevated indicating hepatic cells’ damage after treatment with NaNO3. The hyperbilirubinemia and the increased serum gamma glutamyl transferase activities suggested the presence of cholestasis in NaNO3 exposed rats. In parallel, a significant increase in malondialdehyde level along with a concomitant decrease in total glutathione content and superoxide dismutase, catalase and glutathione peroxidase activities were observed in the liver after NaNO3 treatment. Furthermore, nitrate caused a significant induction of DNA fragmentation. These modifications in NaNO3-treated rats corresponded histologically with hepatocellular necrosis and mononuclear cells infiltration. H. hirta supplementation showed a remarkable amelioration of the abnormalities cited above. Conclusion The results concluded that the treatment with H. hirta had a significant role in protecting the animals from nitrate-induced liver dysfunction.
- Antioxidant /
- Hyparrhenia hirta /
- Liver /
- Nitrate toxicity /
- Oxidative stress /
- Rat

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Nitrate-induced Biochemical and Histopathological Changes in the Liver of Rats:Ameliorative Effect of Hyparrhenia hirta

doi: 10.3967/bes2014.105

基金项目: the ministry of higher education and scientific research in Tunisia (UR/11 ES70)

刊出日期: 2014-09-20

关键词:

Antioxidant /

Liver /

摘要: Objective The present study investigated the protective role of Hyparrhenia hirta (H. hirta) against sodium nitrate (NaNO3)-induced hepatoxicity. Methods Male Wistar rats were randomly divided into three groups:a control group and two treated groups during 50 d with NaNO3 administered either alone in drinking water or co-administered with H. hirta. Results NaNO3 treatment induced a significant increase in serum levels of glucose, total cholesterol and triglyceride while serum total protein level decreased significantly. Transaminases and lactate deshydrogenase activities in serum were elevated indicating hepatic cells’ damage after treatment with NaNO3. The hyperbilirubinemia and the increased serum gamma glutamyl transferase activities suggested the presence of cholestasis in NaNO3 exposed rats. In parallel, a significant increase in malondialdehyde level along with a concomitant decrease in total glutathione content and superoxide dismutase, catalase and glutathione peroxidase activities were observed in the liver after NaNO3 treatment. Furthermore, nitrate caused a significant induction of DNA fragmentation. These modifications in NaNO3-treated rats corresponded histologically with hepatocellular necrosis and mononuclear cells infiltration. H. hirta supplementation showed a remarkable amelioration of the abnormalities cited above. Conclusion The results concluded that the treatment with H. hirta had a significant role in protecting the animals from nitrate-induced liver dysfunction.

English Abstract

Nitrate-induced Biochemical and Histopathological Changes in the Liver of Rats:Ameliorative Effect of Hyparrhenia hirta

Animal Physiology Laboratory, UR/11 ES70, Sfax Faculty of Sciences, University of Sfax, BP1171, Sfax 3000, Tunisia

Laboratory of Human Molecular Genetics, Faculty of Medicine, Sfax 3029, Tunisia

Laboratory of Chemistry, Sfax Faculty of Sciences, University of Sfax, BP1171, Sfax 3000, Tunisia

Laboratory of Biochemistry, CHU Hedi Chaker, Sfax 3029, Tunisia；5. Histopathology Laboratory, University of Sfax, CHU Habib Bourguiba, Sfax 3029, Tunisia

Funds: the ministry of higher education and scientific research in Tunisia (UR/11 ES70)

Publish Date: 2014-09-20

Keywords:

Abstract: Objective The present study investigated the protective role of Hyparrhenia hirta (H. hirta) against sodium nitrate (NaNO3)-induced hepatoxicity. Methods Male Wistar rats were randomly divided into three groups:a control group and two treated groups during 50 d with NaNO3 administered either alone in drinking water or co-administered with H. hirta. Results NaNO3 treatment induced a significant increase in serum levels of glucose, total cholesterol and triglyceride while serum total protein level decreased significantly. Transaminases and lactate deshydrogenase activities in serum were elevated indicating hepatic cells’ damage after treatment with NaNO3. The hyperbilirubinemia and the increased serum gamma glutamyl transferase activities suggested the presence of cholestasis in NaNO3 exposed rats. In parallel, a significant increase in malondialdehyde level along with a concomitant decrease in total glutathione content and superoxide dismutase, catalase and glutathione peroxidase activities were observed in the liver after NaNO3 treatment. Furthermore, nitrate caused a significant induction of DNA fragmentation. These modifications in NaNO3-treated rats corresponded histologically with hepatocellular necrosis and mononuclear cells infiltration. H. hirta supplementation showed a remarkable amelioration of the abnormalities cited above. Conclusion The results concluded that the treatment with H. hirta had a significant role in protecting the animals from nitrate-induced liver dysfunction.

Citation:

Hanen BOUAZIZ-KETATA, Ghada BEN SALAH, Hichem BEN SALAH, Rim MARREKCHI, Kamel JAMOUSSI, Tahia BOUDAWARA, Faiza FAKHFEKH, Najiba ZEGHAL. Nitrate-induced Biochemical and Histopathological Changes in the Liver of Rats:Ameliorative Effect of Hyparrhenia hirta[J]. Biomedical and Environmental Sciences, 2014, 27(9): 695-706. doi: 10.3967/bes2014.105