PEI Xiao Yan; YAN Lin; ZHU Jiang Hui; LI Ning; GUO Yun Chang; FU Ping; JIA Hua Yun; ZHANG Xiu Li; YANG Xiao Rong; YANG Da Jin

doi:10.3967/bes2016.011

The Survey ofCronobacterspp. (formerlyEnterbacter sakazakii) in Infant and Follow-up Powdered Formula in China in 2012

PEI Xiao Yan, YAN Lin, ZHU Jiang Hui, LI Ning, GUO Yun Chang, FU Ping, JIA Hua Yun, ZHANG Xiu Li, YANG Xiao Rong, YANG Da Jin

文章导航 > Biomedical and Environmental Sciences > 2016 > 29(2): 99-106

PEI Xiao Yan, YAN Lin, ZHU Jiang Hui, LI Ning, GUO Yun Chang, FU Ping, JIA Hua Yun, ZHANG Xiu Li, YANG Xiao Rong, YANG Da Jin. The Survey ofCronobacterspp. (formerlyEnterbacter sakazakii) in Infant and Follow-up Powdered Formula in China in 2012[J]. Biomedical and Environmental Sciences, 2016, 29(2): 99-106. doi: 10.3967/bes2016.011

Citation:

PEI Xiao Yan, YAN Lin, ZHU Jiang Hui, LI Ning, GUO Yun Chang, FU Ping, JIA Hua Yun, ZHANG Xiu Li, YANG Xiao Rong, YANG Da Jin. The Survey ofCronobacterspp. (formerlyEnterbacter sakazakii) in Infant and Follow-up Powdered Formula in China in 2012[J]. Biomedical and Environmental Sciences, 2016, 29(2): 99-106. doi: 10.3967/bes2016.011

doi: 10.3967/bes2016.011

基金项目: This study was supported by National High-tech R&D Program of China (863 Program)(2012AA101603)

The Survey ofCronobacterspp. (formerlyEnterbacter sakazakii) in Infant and Follow-up Powdered Formula in China in 2012

China National Center for Food Safety Risk Assessment, Beijing 100022, China
Hunan Province Center for Disease Control and Prevention, Changsha 410000, Hunan, China
Henan Province Center for Disease Control and Prevention, Zhengzhou 450000, Henan, China
Sichuan Province Center for Disease Control and Prevention, Chengdu 610000, Sichuan, China

Funds: This study was supported by National High-tech R&D Program of China (863 Program)(2012AA101603)

摘要: ObjectiveTo determineCronobacterspp.contamination in infant and follow-up powdered formula in China. MethodsAll of 2282 samples were collected from theretailmarkets in China from January 2012 to December 2012, and analyzed forCronobacter spp.by theChineseNationalFood Safety Standard. Characterization of the isolates was analyzed by pulsed-field gel electrophoresis (PFGE) withXbaI and SpeI restriction enzymes. ResultsCronobacterspp. strains were isolated from 25 samples, and the positive rates ininfant powdered formulas and follow-up powdered formulas were 0.90% (10/1011) and 1.18% (15/1271), respectively. Analysis of variable data regarding different purchasing store formats, seasonality, and production locations as well as comparison of infant versus follow-up formulas did not reveal statistically significant factors. During the sampling period, one of six surveillance zones did exhibit a statistically significant trend towards higher positive rate. PFGE characterization ofCronobacterspp.to elucidate genetic diversity revealed only three pairs ofCronobacterspp.out of 25 having the same PFGE patterns. ConclusionThe current investigation indicated a lower positive rate ofCronobacterspp.in the powdered formula in China.This evidence suggested contamination originating from multiple different sources during the manufacturing process.
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Abstract: ObjectiveTo determineCronobacterspp.contamination in infant and follow-up powdered formula in China. MethodsAll of 2282 samples were collected from theretailmarkets in China from January 2012 to December 2012, and analyzed forCronobacter spp.by theChineseNationalFood Safety Standard. Characterization of the isolates was analyzed by pulsed-field gel electrophoresis (PFGE) withXbaI and SpeI restriction enzymes. ResultsCronobacterspp. strains were isolated from 25 samples, and the positive rates ininfant powdered formulas and follow-up powdered formulas were 0.90% (10/1011) and 1.18% (15/1271), respectively. Analysis of variable data regarding different purchasing store formats, seasonality, and production locations as well as comparison of infant versus follow-up formulas did not reveal statistically significant factors. During the sampling period, one of six surveillance zones did exhibit a statistically significant trend towards higher positive rate. PFGE characterization ofCronobacterspp.to elucidate genetic diversity revealed only three pairs ofCronobacterspp.out of 25 having the same PFGE patterns. ConclusionThe current investigation indicated a lower positive rate ofCronobacterspp.in the powdered formula in China.This evidence suggested contamination originating from multiple different sources during the manufacturing process.
- Cronobacterspp. /
- Contamination /
- PFGE /
- Powdered formula

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The Survey ofCronobacterspp. (formerlyEnterbacter sakazakii) in Infant and Follow-up Powdered Formula in China in 2012

doi: 10.3967/bes2016.011

基金项目: This study was supported by National High-tech R&D Program of China (863 Program)(2012AA101603)

刊出日期: 2016-02-20

关键词:

Cronobacterspp. /

Contamination /

PFGE /

Powdered formula

摘要: ObjectiveTo determineCronobacterspp.contamination in infant and follow-up powdered formula in China. MethodsAll of 2282 samples were collected from theretailmarkets in China from January 2012 to December 2012, and analyzed forCronobacter spp.by theChineseNationalFood Safety Standard. Characterization of the isolates was analyzed by pulsed-field gel electrophoresis (PFGE) withXbaI and SpeI restriction enzymes. ResultsCronobacterspp. strains were isolated from 25 samples, and the positive rates ininfant powdered formulas and follow-up powdered formulas were 0.90% (10/1011) and 1.18% (15/1271), respectively. Analysis of variable data regarding different purchasing store formats, seasonality, and production locations as well as comparison of infant versus follow-up formulas did not reveal statistically significant factors. During the sampling period, one of six surveillance zones did exhibit a statistically significant trend towards higher positive rate. PFGE characterization ofCronobacterspp.to elucidate genetic diversity revealed only three pairs ofCronobacterspp.out of 25 having the same PFGE patterns. ConclusionThe current investigation indicated a lower positive rate ofCronobacterspp.in the powdered formula in China.This evidence suggested contamination originating from multiple different sources during the manufacturing process.

English Abstract

The Survey ofCronobacterspp. (formerlyEnterbacter sakazakii) in Infant and Follow-up Powdered Formula in China in 2012

China National Center for Food Safety Risk Assessment, Beijing 100022, China

Hunan Province Center for Disease Control and Prevention, Changsha 410000, Hunan, China

Henan Province Center for Disease Control and Prevention, Zhengzhou 450000, Henan, China

Sichuan Province Center for Disease Control and Prevention, Chengdu 610000, Sichuan, China

Funds: This study was supported by National High-tech R&D Program of China (863 Program)(2012AA101603)

Publish Date: 2016-02-20

Keywords:

Abstract: ObjectiveTo determineCronobacterspp.contamination in infant and follow-up powdered formula in China. MethodsAll of 2282 samples were collected from theretailmarkets in China from January 2012 to December 2012, and analyzed forCronobacter spp.by theChineseNationalFood Safety Standard. Characterization of the isolates was analyzed by pulsed-field gel electrophoresis (PFGE) withXbaI and SpeI restriction enzymes. ResultsCronobacterspp. strains were isolated from 25 samples, and the positive rates ininfant powdered formulas and follow-up powdered formulas were 0.90% (10/1011) and 1.18% (15/1271), respectively. Analysis of variable data regarding different purchasing store formats, seasonality, and production locations as well as comparison of infant versus follow-up formulas did not reveal statistically significant factors. During the sampling period, one of six surveillance zones did exhibit a statistically significant trend towards higher positive rate. PFGE characterization ofCronobacterspp.to elucidate genetic diversity revealed only three pairs ofCronobacterspp.out of 25 having the same PFGE patterns. ConclusionThe current investigation indicated a lower positive rate ofCronobacterspp.in the powdered formula in China.This evidence suggested contamination originating from multiple different sources during the manufacturing process.

Citation:

PEI Xiao Yan, YAN Lin, ZHU Jiang Hui, LI Ning, GUO Yun Chang, FU Ping, JIA Hua Yun, ZHANG Xiu Li, YANG Xiao Rong, YANG Da Jin. The Survey ofCronobacterspp. (formerlyEnterbacter sakazakii) in Infant and Follow-up Powdered Formula in China in 2012[J]. Biomedical and Environmental Sciences, 2016, 29(2): 99-106. doi: 10.3967/bes2016.011