ZHANG Juan; YIN LiHong; LIANG GeYu; LIU Ran; FAN KaiHong; PU YuePu

doi:10.3967/0895-3988.2011.03.014

Detection of CYP2E1, a Genetic Biomarker of Susceptibility to Benzene Metabolism Toxicity in Immortal Human Lymphocytes Derived from the Han Chinese Population

ZHANG Juan, YIN LiHong, LIANG GeYu, LIU Ran, FAN KaiHong, PU YuePu

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(3): 300-309

ZHANG Juan, YIN LiHong, LIANG GeYu, LIU Ran, FAN KaiHong, PU YuePu. Detection of CYP2E1, a Genetic Biomarker of Susceptibility to Benzene Metabolism Toxicity in Immortal Human Lymphocytes Derived from the Han Chinese Population[J]. Biomedical and Environmental Sciences, 2011, 24(3): 300-309. doi: 10.3967/0895-3988.2011.03.014

Citation:

ZHANG Juan, YIN LiHong, LIANG GeYu, LIU Ran, FAN KaiHong, PU YuePu. Detection of CYP2E1, a Genetic Biomarker of Susceptibility to Benzene Metabolism Toxicity in Immortal Human Lymphocytes Derived from the Han Chinese Population[J]. Biomedical and Environmental Sciences, 2011, 24(3): 300-309. doi: 10.3967/0895-3988.2011.03.014

doi: 10.3967/0895-3988.2011.03.014

基金项目: the National Natural Science Foundation of China(30671731%30901168)%the Doctoral Program of Higher Education of China(20070286069)

Detection of CYP2E1, a Genetic Biomarker of Susceptibility to Benzene Metabolism Toxicity in Immortal Human Lymphocytes Derived from the Han Chinese Population

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China

Funds: the National Natural Science Foundation of China(30671731%30901168)%the Doctoral Program of Higher Education of China(20070286069)

摘要: Objective Cytochrome P450 2E1 (CYP2E1) is an important metabolizing enzyme involved in oxidative stress responses to benzene, a chemical associated with bone marrow toxicity and leukemia. We aimed to identify the CYP2E1 genetic biomarkers of susceptibility to benzene toxicity in support of environmental and occupational exposure prevention, and to test whether a model using immortal human lymphocytes might be an efficient tool for detecting genetic biomarkers.Methods Immortalized human lymphocyte cell lines with independent genotypes on four CYP2E1 SNP sites were induced with 0.01% phenol, a metabolite of benzene. CYP2E1 gene function was evaluated by mRNA expression and enzyme activity. DNA damage was measured by Single-Cell Gel Electrophoresis (SCGE).Results Among the four SNPs, cells with rs2070673TT and rs2030920CC showed higher levels of CYP2E1 transcription and enzymatic activity than the other genotypes in the same SNP site. Cells with higher gene expression genotypes also showed higher comet rates compared with lower gene expression genotypes.Conclusion These results suggest that CYP2E1 rs2070673 and rs2030920 might be the genetic biomarkers of susceptibility to benzene toxicity and that the immortalized human lymphocytes model might be an efficient tool for the detection of genetic biomarkers of susceptibility to chemicals.
- /
- /
- /
- /
- /
Abstract: Objective Cytochrome P450 2E1 (CYP2E1) is an important metabolizing enzyme involved in oxidative stress responses to benzene, a chemical associated with bone marrow toxicity and leukemia. We aimed to identify the CYP2E1 genetic biomarkers of susceptibility to benzene toxicity in support of environmental and occupational exposure prevention, and to test whether a model using immortal human lymphocytes might be an efficient tool for detecting genetic biomarkers.Methods Immortalized human lymphocyte cell lines with independent genotypes on four CYP2E1 SNP sites were induced with 0.01% phenol, a metabolite of benzene. CYP2E1 gene function was evaluated by mRNA expression and enzyme activity. DNA damage was measured by Single-Cell Gel Electrophoresis (SCGE).Results Among the four SNPs, cells with rs2070673TT and rs2030920CC showed higher levels of CYP2E1 transcription and enzymatic activity than the other genotypes in the same SNP site. Cells with higher gene expression genotypes also showed higher comet rates compared with lower gene expression genotypes.Conclusion These results suggest that CYP2E1 rs2070673 and rs2030920 might be the genetic biomarkers of susceptibility to benzene toxicity and that the immortalized human lymphocytes model might be an efficient tool for the detection of genetic biomarkers of susceptibility to chemicals.
- Cytochrome P450 2E1 /
- Single-nucleotide polymorphism /
- Genetic biomarker /
- Human immortalized B lymphocytes /
- Benzene /
- Phenol

[1]	QIN Jia Bi, LUO Liu, SUN Meng Ting, HUANG Peng, WANG Ting Ting, ZHANG Sen Mao, LI Jin Qi, LI Yi Huan, CHEN Le Tao, DIAO Jing Yi, ZHU Ping. Association between Maternal Drug Use and Cytochrome P450 Genetic Polymorphisms and the Risk of Congenital Heart Defects in Offspring . Biomedical and Environmental Sciences, 2022, 35(1): 45-57. doi: 10.3967/bes2022.006
[2]	LIU Chen Geng, MENG Shuang, LI Ying, LU Yao, ZHAO Yue, WANG Pei Chang. MicroRNA-135a in ABCA1-labeled Exosome is a Serum Biomarker Candidate for Alzheimer’s Disease . Biomedical and Environmental Sciences, 2021, 34(1): 19-28. doi: 10.3967/bes2021.004
[3]	ZHANG Liang, WU Zhen An. MicroRNA-378a-3p Downregulation as a Novel Biomarker with Poor Clinical Outcomes in Cervical Cancer . Biomedical and Environmental Sciences, 2021, 34(3): 213-221. doi: 10.3967/bes2021.026
[4]	ZHANG Xiao Min, LIU Jing, CAO Min, YANG Ting Ting, WANG Ya Qi, HOU Yu Li, SONG Qiao, CUI Yu Ting, WANG Pei Chang. TREM2: A Novel Potential Biomarker of Alzheimer’s Disease . Biomedical and Environmental Sciences, 2021, 34(9): 719-724. doi: 10.3967/bes2021.099
[5]	WANG Tong Shuai, SONG Bo, SUN Qing Hua, LIN Yong Xin, SUN Yuan, SUN Pin, JIA Xiao Dong, William W. Au, MEI Can Hua, XIA Zhao Lin. Occupational Health Risk Assessment of Benzene, Toluene, and Xylene in Shanghai . Biomedical and Environmental Sciences, 2021, 34(4): 290-298. doi: 10.3967/bes2021.038
[6]	XIAN Hong Yi, CHEN Ying, ZHANG Jia Ying, TANG Mei Lin, LIAN Zhen Wei, JIANG Ran, HU Zu Qing, LI Yan Feng, HU Da Lin. Exosomes Derived from Hydroquinone-transformed Human Bronchial Epithelial Cells Inhibited Recipient Cell Apoptosis by transferring miR-221 . Biomedical and Environmental Sciences, 2021, 34(7): 520-527. doi: 10.3967/bes2021.072
[7]	WANG Chao, XU Yong Jun, SHI Ying, WANG Chong, DUAN Lian, GU Wen, RUAN Hong Jie, ZHANG Shao Ping, ZHI Hong, KONG Jian, ZHANG Li Xia, ZHANG Ming, ZHANG Hong Wei. Verification on the Developmental Toxicity of Short-term Exposure to Phenol in Rats . Biomedical and Environmental Sciences, 2020, 33(6): 403-413. doi: 10.3967/bes2020.055
[8]	WANG Bo Shen, HAN Lei, YANG Dan Dan, ZHANG Juan, YIN Li Hong, ZHANG Meng Ying, XU Kai, XING Cai Hong, ZHU Bao Li, PU Yue Pu. Impact of Enterprise Ownership and Size on Registered Hematotoxicity in Benzene-exposed Workers . Biomedical and Environmental Sciences, 2018, 31(12): 917-921. doi: 10.3967/bes2018.125
[9]	XU Jing Jing, JIANG Lin, XU Lian Jun, GAO Zhan, ZHAO Xue Yan, ZHANG Yin, SONG Ying, LIU Ru, SUN Kai, GAO Run Lin, XU Bo, SONG Lei, YUAN Jin Qing. Association of CDKN2B-AS1 Polymorphisms with Premature Triple-vessel Coronary Disease and Their Sex Specificity in the Chinese Population . Biomedical and Environmental Sciences, 2018, 31(11): 787-796. doi: 10.3967/bes2018.106
[10]	ZHANG Yao Dong, TAN Li Na, ZHANG Xiao Ling, WEI Hal Yan, XIONG Hao, HU Qun. Meta-analysis of Cytochrome P4501A1 Mspl Gene Polymorphism and Childhood Acute Leukemia . Biomedical and Environmental Sciences, 2011, 24(6): 683-687. doi: 10.3967/0895-3988.2011.06.014
[11]	JIAN-LIN LOU, ZHI-JIAN CHEN, JIANG WEI, JI-LIANG HE, LI-FEN JIN, SHI-JIE CHEN, WEI ZHENG, SHI-JIE XU. Response of Lymphocytes to Radiation in Untreated Breast Cancer Patients as Detected with Three Different Genetic Assays . Biomedical and Environmental Sciences, 2008, 21(6): 499-508.
[12]	MIN YANG, CHANG-CHUN QIU, WEI CHEN, LING-LING XU, MIAO YU, HONG-DING XIANG. Identification of a Regulatory Single Nucleotide Polymorphism in the Adiponectin (APM1) Gene Associated with Type2 Diabetes in Han Nationality . Biomedical and Environmental Sciences, 2008, 21(6): 454-459.
[13]	RONG CHENG, JIAN-LONG WANG, WEI-XIAN ZHANG. Reductive Dechlorination of p-Chlorophenol by Nanoscale Iron . Biomedical and Environmental Sciences, 2007, 20(5): 410-413.
[14]	XIU-LI CHANG, TAI-YI JIN, YUAN-FEN ZHOU. Metallothionein 1 Isoform Gene Expression Induced by Cadmium in Human Peripheral Blood Lymphocytes . Biomedical and Environmental Sciences, 2006, 19(2): 104-109.
[15]	XIA-MIN HU, SHI-ZHEN SONG, FANG-LI YE, LI-WEN LIU. High-performance Liquid Chromatographic Determination of Urinary Trans, Trans-Muconic Acid Excreted by Workers Occupationally Exposed to Benzene . Biomedical and Environmental Sciences, 2006, 19(4): 292-296.
[16]	YING-XU CHEN, HE Liu, HUA-LIN CHEN. Characterization of Phenol Biodegradation by Comamonas testosteroni ZD4-1 and Pseudomonas aeruginosa ZD4-3 . Biomedical and Environmental Sciences, 2003, 16(2): 163-172.
[17]	SUN Xu-Ming, ZHANG Xiang-Hong, WANG Hui-yan, CAO WEN-JUN, YAN Xia, ZUO Lian-fu, WANG JUN-LING, WANG Feng-Rong. Effects of Sterigmatocystin, Deoxynivalenol and Aflatoxin G1 on Apoptosis of Human Peripheral Blood Lymphocytes in vitro . Biomedical and Environmental Sciences, 2002, 15(2): 145-152.
[18]	SUN Hong-xia, ZHANG KUI-XING, DU WEI-NAN, SHI JIN-XIU, JIANG ZHENG-WEN, SUN HAO, ZUO JIN, HUANG WEI, CHEN ZHU, SHEN YAN, YAO Zhi-Jian, QIANG Bo-Qin, FANG FU-DE. Single Nucleotide Polymorphisms in CAPN10 Gene of Chinese People and Its Correlation With Type 2 Diabetes Mellitus in Han People of Northern China . Biomedical and Environmental Sciences, 2002, 15(1): 75-82.
[19]	LIN BIN, HENK W.VAN VERSEVELD, WILFRED F.M.R(O)LING. Microbial Aspects of Anaerobic BTEX Degradation . Biomedical and Environmental Sciences, 2002, 15(2): 130-144.
[20]	HU WEN-YU, LI YA-WEI, HOU YAN-NING, HE KAN, CHEN JIE-FU, BUT PAUL PUI-HAY, ZHU XIU-YUAN. The Induction of Liver Microsomal Cytochrome P450 by Glycyrrhiza uralensis and Glycyrrhetinic acid in Mice . Biomedical and Environmental Sciences, 1999, 12(1): 10-14.

点击查看大图

计量

文章访问数: 1475
HTML全文浏览量: 346
PDF下载量: 66
被引次数: 0

Detection of CYP2E1, a Genetic Biomarker of Susceptibility to Benzene Metabolism Toxicity in Immortal Human Lymphocytes Derived from the Han Chinese Population

doi: 10.3967/0895-3988.2011.03.014

基金项目: the National Natural Science Foundation of China(30671731%30901168)%the Doctoral Program of Higher Education of China(20070286069)

刊出日期: 2011-06-20

关键词:

Cytochrome P450 2E1 /

Single-nucleotide polymorphism /

Genetic biomarker /

Human immortalized B lymphocytes /

Benzene /

Phenol

摘要: Objective Cytochrome P450 2E1 (CYP2E1) is an important metabolizing enzyme involved in oxidative stress responses to benzene, a chemical associated with bone marrow toxicity and leukemia. We aimed to identify the CYP2E1 genetic biomarkers of susceptibility to benzene toxicity in support of environmental and occupational exposure prevention, and to test whether a model using immortal human lymphocytes might be an efficient tool for detecting genetic biomarkers.Methods Immortalized human lymphocyte cell lines with independent genotypes on four CYP2E1 SNP sites were induced with 0.01% phenol, a metabolite of benzene. CYP2E1 gene function was evaluated by mRNA expression and enzyme activity. DNA damage was measured by Single-Cell Gel Electrophoresis (SCGE).Results Among the four SNPs, cells with rs2070673TT and rs2030920CC showed higher levels of CYP2E1 transcription and enzymatic activity than the other genotypes in the same SNP site. Cells with higher gene expression genotypes also showed higher comet rates compared with lower gene expression genotypes.Conclusion These results suggest that CYP2E1 rs2070673 and rs2030920 might be the genetic biomarkers of susceptibility to benzene toxicity and that the immortalized human lymphocytes model might be an efficient tool for the detection of genetic biomarkers of susceptibility to chemicals.

English Abstract

Detection of CYP2E1, a Genetic Biomarker of Susceptibility to Benzene Metabolism Toxicity in Immortal Human Lymphocytes Derived from the Han Chinese Population

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China

Funds: the National Natural Science Foundation of China(30671731%30901168)%the Doctoral Program of Higher Education of China(20070286069)

Publish Date: 2011-06-20

Keywords:

Abstract: Objective Cytochrome P450 2E1 (CYP2E1) is an important metabolizing enzyme involved in oxidative stress responses to benzene, a chemical associated with bone marrow toxicity and leukemia. We aimed to identify the CYP2E1 genetic biomarkers of susceptibility to benzene toxicity in support of environmental and occupational exposure prevention, and to test whether a model using immortal human lymphocytes might be an efficient tool for detecting genetic biomarkers.Methods Immortalized human lymphocyte cell lines with independent genotypes on four CYP2E1 SNP sites were induced with 0.01% phenol, a metabolite of benzene. CYP2E1 gene function was evaluated by mRNA expression and enzyme activity. DNA damage was measured by Single-Cell Gel Electrophoresis (SCGE).Results Among the four SNPs, cells with rs2070673TT and rs2030920CC showed higher levels of CYP2E1 transcription and enzymatic activity than the other genotypes in the same SNP site. Cells with higher gene expression genotypes also showed higher comet rates compared with lower gene expression genotypes.Conclusion These results suggest that CYP2E1 rs2070673 and rs2030920 might be the genetic biomarkers of susceptibility to benzene toxicity and that the immortalized human lymphocytes model might be an efficient tool for the detection of genetic biomarkers of susceptibility to chemicals.

Citation:

ZHANG Juan, YIN LiHong, LIANG GeYu, LIU Ran, FAN KaiHong, PU YuePu. Detection of CYP2E1, a Genetic Biomarker of Susceptibility to Benzene Metabolism Toxicity in Immortal Human Lymphocytes Derived from the Han Chinese Population[J]. Biomedical and Environmental Sciences, 2011, 24(3): 300-309. doi: 10.3967/0895-3988.2011.03.014

全文HTML

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

Biomedical and Environmental Sciences ISSN 0895-3988 CN 11-2816 / Q

Publish Under the Auspices of Chinese Center for Disease Control and Prevention

Distributed by Chinese Center for Disease Control and Prevention

Quick Links

Supported by Beijing Renhe Information Technology Co. Ltd E-mail: info@rhhz.net

doi: 10.3967/0895-3988.2011.03.014

Detection of CYP2E1, a Genetic Biomarker of Susceptibility to Benzene Metabolism Toxicity in Immortal Human Lymphocytes Derived from the Han Chinese Population

计量

出版历程

Detection of CYP2E1, a Genetic Biomarker of Susceptibility to Benzene Metabolism Toxicity in Immortal Human Lymphocytes Derived from the Han Chinese Population

doi: 10.3967/0895-3988.2011.03.014

English Abstract

Detection of CYP2E1, a Genetic Biomarker of Susceptibility to Benzene Metabolism Toxicity in Immortal Human Lymphocytes Derived from the Han Chinese Population

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China

全文HTML

目录